Results 131 to 140 of about 6,913 (153)

GCN2 monitors mRNA translation termination

Molecular Cell
Controlling mRNA translation is critical for proper protein production. Although translation initiation and elongation regulations are becoming increasingly clear, whether and how translation termination is monitored remains poorly understood. Using an acute protein degradation system coupled with phenotypic rescue via ectopic expression, here we show ...
Wenqian Hu
exaly   +3 more sources

GCN2 adapts protein synthesis to scavenging-dependent growth

Cell Systems, 2022
Pancreatic cancer cells with limited access to free amino acids can grow by scavenging extracellular protein. In a murine model of pancreatic cancer, we performed a genome-wide CRISPR screen for genes required for scavenging-dependent growth. The screen identified key mediators of macropinocytosis, peripheral lysosome positioning, endosome-lysosome ...
Michel, Nofal, Tim, Wang, Lifeng, Yang, Connor S R, Jankowski, Sophia, Hsin-Jung Li, Seunghun, Han, Lance, Parsons, Alexander N, Frese, Zemer, Gitai, Tracy G, Anthony, Martin, Wühr, David M, Sabatini, Joshua D, Rabinowitz   +12 more
openaire   +2 more sources

Genetic evidence for functional specificity of the yeast GCN2 kinase

MGG Molecular & General Genetics, 1996
In yeast the GCN2 kinase mediates translational control of GCN4 by phosphorylating the alpha subunit of eIF-2 in response to extracellular amino acid limitation. Although phosphorylation of eIF-2 alpha has been shown to inhibit global protein synthesis, amino acid starvation results in a specific activation effect on GCN4 mRNA translation.
N. Tavernarakis, G. Thireos, N. Tavernarakis   +2 more
openaire   +2 more sources

GCN2 activation and eIF2α phosphorylation in the maturation of mouse oocytes

Biochemical and Biophysical Research Communications, 2009
GCN2 is one of the four mammalian kinases that phosphorylate the alpha subunit of the translation initiation factor 2 (eIF2alpha) in a variety of stress situations, resulting in protein synthesis inhibition. GCN2 is involved in regulating metabolism, feeding behavior and memory in rodents. We show here that, relative to other cells, the beta isoform of
Viviane S, Alves, Fabiana L, Motta, Martín, Roffé, Arthur, Delamano, João B, Pesquero, Beatriz A, Castilho   +5 more
openaire   +2 more sources

An In Vitro System to Study Mammalian GCN2 Regulation

General control non-derepressible 2 (GCN2) is an eIF2α kinase responsible for eliciting the integrated stress response (ISR) under amino acid starvation conditions. How GCN2 can sense amino acid starvation and become active in mammalian systems is not fully understood as there are a plethora of protein cofactors and post-translational modifications ...
Glenn R. Masson, Vanesa Vinciauskaite
openaire   +2 more sources

GCN2-Mediated Integrated Stress Response Attenuates Periodontitis

Journal of Dental Research
The integrated stress response (ISR), regulated by general control nonderepressible 2 (GCN2), is essential for maintaining tissue homeostasis, yet its role in periodontitis remains poorly understood. Here, through transcriptomic analysis and immunohistochemistry of gingival biopsies from patients and a ligature-induced mouse ...
D. Huang, Q. Li, S. Peng, Y. Li, Q. Yin, X. Yang, X. Gan, Y. Wang, X. Li, Y. Zhao, Y. Guo, W. Lin, Y. Li, N. Feng, Q. Yuan   +14 more
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Characterisation of Small-Molecule GCN2 Modulators

Diversely derived ATP-competitive inhibitors have shown the ability to paradoxically activate the kinase GCN2. This project investigates clinically approved BRAF inhibitors dabrafenib and encorafenib, that are used as chemotherapeutic treatments for V600E mutant BRAF tumours.
openaire   +1 more source

GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment

Cell Metabolism, 2022
Nicolas Skuli, Romain Riscal, M Celeste Simon   +2 more
exaly  

GCN2 regulation of mTORC2 and mitochondrial metabolism

Highly proliferating cells require substantial nutrient availability to maintain growth and survival. It is important to understand the regulation of nutrient-sensing pathways that are critical for cellular homeostasis. Glutamine is vital in the cell because it serves as a major nitrogen source and alternative carbon source for energy production and ...
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The role of GCN2 in somatic maintenance and ageing

Ageing is thought to occur because our cells accumulate damage over time. We do not yet know which type of damage is responsible for ageing, but we do know that our diet can affect the rate of damage accumulation and as a result our lifespan. Dietary protein, which is made up of amino acids, is especially important. My research explores how fruit flies
openaire   +1 more source