Results 51 to 60 of about 6,913 (153)

Role of activating transcription factor 4 in the hepatic response to amino acid depletion by asparaginase

Scientific Reports, 2017
The anti-leukemic agent asparaginase activates the integrated stress response (ISR) kinase GCN2 and inhibits signaling via mechanistic target of rapamycin complex 1 (mTORC1).
Rana J. T. Al-Baghdadi, Inna A. Nikonorova, Emily T. Mirek, Yongping Wang, Jinhee Park, William J. Belden, Ronald C. Wek, Tracy G. Anthony   +7 more
doaj   +1 more source

Nucleofection induces transient eIF2α phosphorylation by GCN2 and PERK [PDF]

Gene Therapy, 2012
Nucleofection permits efficient transfection even with difficult cell types such as primary and non-dividing cells, and is used to deliver various nucleic acids, including DNA, mRNA, and small interfering RNA. Unlike DNA and small interfering RNA, mRNA is subject to rapid degradation, which necessitates instant early translation following mRNA delivery.
Anderson, Bart R., Karikó, Katalin, Weissman, Drew   +2 more
openaire   +2 more sources

Activation of GCN2 in UV-Irradiated Cells Inhibits Translation [PDF]

Current Biology, 2002
Mammalian cells subjected to ultraviolet (UV) irradiation actively repress DNA replication, transcription, and mRNA translation. While the effects of UV irradiation on DNA replication and transcription have been extensively studied, the mechanism(s) responsible for translational repression are poorly understood.Here, we demonstrate that UV irradiation ...
Deng, Jing, Harding, Heather P., Raught, Brian, Gingras, Anne-Claude, Berlanga, Juan Jose, Scheuner, Donalyn, Kaufman, Randal J., Ron, David, Sonenberg, Nahum   +8 more
openaire   +2 more sources

Amino Acid Sensor Kinase Gcn2 Is Required for Conidiation, Secondary Metabolism, and Cell Wall Integrity in the Taxol-Producer Pestalotiopsis microspora

Frontiers in Microbiology, 2017
The canonical Gcn2/Cpc1 kinase in fungi coordinates the expression of target genes in response to amino acid starvation. To investigate its possible role in secondary metabolism, we characterized a gcn2 homolog in the taxol-producing fungus ...
Dan Wang, Oren Akhberdi, Xiaoran Hao, Xi Yu, Longfei Chen, Yanjie Liu, Xudong Zhu   +6 more
doaj   +1 more source

Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2‐dependent and GCN2‐independent pathways [PDF]

The FEBS Journal, 2009
Evidence has accumulated that amino acids play an important role in controlling gene expression. Nevertheless, two components of the amino acid control of gene expression are not yet completely understood in mammals: (a) the target genes and biological processes regulated by amino acid availability, and (b) the signaling pathways that mediate the amino
Deval, Christiane, Chaveroux, Cédric, Maurin, Anne-Catherine, Cherasse, Yoan, Parry, Laurent, Carraro, Valerie, Milenkovic, Dragan, Ferrara, Marc, Bruhat, Alain, Jousse, Céline, Fafournoux, Pierre   +10 more
openaire   +4 more sources

The GCN2 inhibitor IMPACT contributes to diet-induced obesity and body temperature control.

PLoS ONE, 2019
IMPACT, a highly conserved protein, is an inhibitor of the eIF2α kinase GCN2. In mammals, it is preferentially expressed in neurons. Knock-down of IMPACT expression in neuronal cells increases basal GCN2 activation and eIF2α phosphorylation and decreases
Catia M Pereira, Renato Filev, Francisco P Dubiela, Bruna B Brandão, Claudio M Queiroz, Raissa G Ludwig, Debora Hipolide, Beatriz M Longo, Luiz E Mello, Marcelo A Mori, Beatriz A Castilho   +10 more
doaj   +1 more source

The ribosomal P-stalk couples amino acid starvation to GCN2 activation in mammalian cells

eLife, 2019
The eukaryotic translation initiation factor 2α (eIF2α) kinase GCN2 is activated by amino acid starvation to elicit a rectifying physiological program known as the Integrated Stress Response (ISR).
Heather P Harding, Adriana Ordonez, Felicity Allen, Leopold Parts, Alison J Inglis, Roger L Williams, David Ron   +6 more
doaj   +1 more source

Recognition of a structural domain (RWDBD) in Gcn1 proteins that interacts with the RWD domain containing proteins

Biology Direct, 2017
The protein Gcn1 (General control non-derepressible 1) is found in virtually all eukaryotes, and is a key component of the general amino acid control signal transduction pathway.
Ramachandran Rakesh, Rangachari Krishnan, Evelyn Sattlegger, Narayanaswamy Srinivasan   +3 more
doaj   +1 more source

Ribosome binding protein GCN1 regulates the cell cycle and cell proliferation and is essential for the embryonic development of mice.

PLoS Genetics, 2020
Amino acids exert many biological functions, serving as allosteric regulators and neurotransmitters, as constituents in proteins and as nutrients. GCN2-mediated phosphorylation of eukaryotic initiation factor 2 alpha (elF2α) restores homeostasis in ...
Hiromi Yamazaki, Shuya Kasai, Junsei Mimura, Peng Ye, Atsushi Inose-Maruyama, Kunikazu Tanji, Koichi Wakabayashi, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Tsubasa Sato, Taku Ozaki, Douglas R Cavener, Masayuki Yamamoto, Ken Itoh   +14 more
doaj   +1 more source

Disrupting ATF4 Expression Mechanisms Provides an Effective Strategy for BRAF-Targeted Melanoma Therapy

iScience, 2020
Summary: BRAF V600 mutation influences cellular signaling pathways for melanoma development. However, the role of oncogenic BRAF in adaptive stress response pathways is not fully understood.
Ikuko Nagasawa, Masaru Koido, Yuri Tani, Satomi Tsukahara, Kazuhiro Kunimasa, Akihiro Tomida   +5 more
doaj   +1 more source