Results 201 to 210 of about 7,371,567 (249)
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Is Gene Amplification RNA-directed?
Nature New Biology, 1973Gene amplification in oocytes has been thought to involve a “reverse transcriptase” like that found in the RNA tumour viruses. Neither the template RNA nor the hybrid intermediates required by this theory can be detected in amplifying ovaries.
Max L. Birnstiel +2 more
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2001
Double minutes (DMs) and homogeneously stained regions (HSRs) provide good examples of phenomena that were originally regarded as cytological oddities but have turned out to be expressions of a fundamental process, called DNA or gene amplification. They arise almost exclusively in transformed and malignant cells.
Orlando J. Miller, Eeva Therman
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Double minutes (DMs) and homogeneously stained regions (HSRs) provide good examples of phenomena that were originally regarded as cytological oddities but have turned out to be expressions of a fundamental process, called DNA or gene amplification. They arise almost exclusively in transformed and malignant cells.
Orlando J. Miller, Eeva Therman
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2006
Since the approval of trastuzumab for the treatment of HER2-positive breast cancer (1), there has been an active debate as to the reproducibility and validity of the FDA-approved assays that detect HER2 abnormalities in the tumor tissue. In this chapter, various clinical assays are compared focusing on practical issues.
Yong-kuk Song +2 more
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Since the approval of trastuzumab for the treatment of HER2-positive breast cancer (1), there has been an active debate as to the reproducibility and validity of the FDA-approved assays that detect HER2 abnormalities in the tumor tissue. In this chapter, various clinical assays are compared focusing on practical issues.
Yong-kuk Song +2 more
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Gene Amplification in Rhizobium
1993Rhizobium species have stimulated the scientific interest due to their ability to interact with plants establishing nitrogen-fixing symbioses. During the symbiotic process, an intimate exchange of chemical signals between the partners results in the expression of specific sets of genes from both bacteria and plant cells.
Ma. Lourdes Girard +6 more
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Gene Amplification and qRT-PCR
2014This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell
Alain Filloux, Cerith Jones
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Gene amplification and tumor progression
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1993Proto-oncogenes are the genes which are most frequently found amplified in human tumor cells. Acquisition of a drug-resistant phenotype by gene amplification is frequent for in-vitro cultured cells but is very rare in human tumors. Proto-oncogenes amplified in human tumors belong essentially to one of three families (erbB, ras, myc) or to the 11q13 ...
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Gene amplification and gene correction in somatic cells
Cell, 1982We used gene transfer to identify frequent genetic rearrangements responsible for activating mutant genes in mammalian cells. We transformed an aprt- tk- cell with a plasmid containing a wild-type aprt gene and a truncated, promoterless tk gene. Transformants that integrate a single copy of this plasmid exhibit the aprt+ phenotype but remain tk-.
Richard Axel, James M. Roberts
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Gene amplification in human gliomas
Glia, 1995AbstractGliomas represent the largest group of primary brain tumors in adults. The astrocytic variants are the most common and the adult forms are histologically stratified into three malignancy grades. Of these glioblastoma is the most common and the most malignant; it has also been best studied by molecular genetics and cytogenetics.
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Esterase gene amplification in Culex pipiens
Insect Molecular Biology, 1997In the mosquito Culex pipiens one of the major resistance mechanisms to organophosphorous pesticides (OPs) is increased detoxification of insecticide. This resistance is the consequence of overproduction of two types of esterases, esterases A and B, coded at two loci, Est-3 (A esterase) and Est-2 (B esterase).
Guillemaud, Thomas +4 more
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Gene amplifications in osteosarcoma—CGH microarray analysis
Genes, Chromosomes and Cancer, 2004AbstractLittle is known about the genomic alterations underlying osteosarcoma. We performed a genomewide high‐resolution gene copy number analysis of 22 osteosarcoma samples using comparative genomic hybridization on a cDNA microarray that contained cDNA clones of about 13,000 genes.
Atiye, Jassu +10 more
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