Results 41 to 50 of about 13,075 (222)

Nef induced transcriptional alteration of the six genes measured by qPCR.

, 2015
Real Time RT-PCR was performed to analyse the expression of the six genes. Fold change in expression upon Nef transfection is presented in respect to control. Except VDAC1 all 5 genes were downregulated by Nef variants.
Sudipti Gupta (721839), Reshu Saxena (130830), Kalyan Mitra (514430), Kavita Singh (182141), Anil Kumar Tripathi (198121), Raj Kamal Tripathi (111947)   +5 more
core   +1 more source

HIV-1-related factors interact with p53 to influence cellular processes

AIDS Research and Therapy, 2023
Human immunodeficiency virus type 1 (HIV-1) is the primary epidemic strain in China. Its genome contains two regulatory genes (tat and rev), three structural genes (gag, pol, and env), and four accessory genes (nef, vpr, vpu, and vif).
Shanling Liu, Ting Guo, Jinwei Hu, Weiliang Huang, Pengfei She, Yong Wu   +5 more
doaj   +1 more source

Expression, purification and biochemical characterisation of the human immunodificiency virus 1 nef gene product [PDF]

European Journal of Biochemistry, 1992
The human immunodeficiency virus 1 (HIV‐1) nef gene encoded by the HIV‐1 isolate lymphadenopathy‐associated virus type 1 was expressed in Escherichia coli under the control of the tac promoter. The protein is found mainly in the soluble part of the bacterial lysate; a simple twocolumn purification scheme has been developed allowing isolation of the ...
Wolber, V., Rensland, H., Brandmeier, B., Sagemann, M., Hoffmann, R., Kalbitzer, H., Wittinghofer, A.   +6 more
openaire   +4 more sources

Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses

, 2010
Since HLA-restricted cytotoxic T-cell responses select specific polymorphisms in HIV-1 sequences and HLA diversity is relatively static in human populations, we investigated the use of peptide epitopes based on sites of HLA-associated adaptation in HIV-1
McKinnon, E., Rebecca Laird, Chopra, A., John, M., Simon Mallal, Almeida, C.M., Keane, N.M., Abha Chopra, David Heckerman, Heckerman, D., Imran Ahmad, Coral-Ann M. Almeida, Elizabeth McKinnon, Carl Kadie, Roberts, S.G., Mina John, Kadie, C., Niamh M. Keane, Laird, R., Steven G. Roberts, Ahmad, I., Mallal, S.   +21 more
core   +1 more source

Overexpression of recombinant HIV-1 Subtype C Tat and Nef in a Salmonella vaccine vector

The Pan African Medical Journal, 2013
Tat and Nef are very important regulatory proteins of HIV-1. They enhance viral replication and down-regulate expression of MHC Class I molecules, respectively. The antigens are now considered to be targets for HIV vaccine development.
Nyasha Chineombe, Maribanyana Lebeko, Mankgopo Kgatle   +2 more
doaj   +1 more source

Signature molecules expressed differentially in a liver disease stage-specific manner by HIV-1 and HCV co-infection. [PDF]

PLoS ONE, 2018
To elucidate HIV-1 co-infection-induced acceleration of HCV liver disease and identify stage-specific molecular signatures, we applied a new high-resolution molecular screen, the Affymetrix GeneChip Human Transcriptome Array (HTA2.0), to HCV-mono- and ...
Amanda Whitmill, Seongcheol Kim, Vivian Rojas, Fahad Gulraiz, Kazi Afreen, Mamta Jain, Meharvan Singh, In-Woo Park   +7 more
doaj   +1 more source

HLA-Associated viral mutations are common in human immunodeficiency virus type 1 elite controllers

, 2009
Elite controllers (EC) of human immunodeficiency virus type 1 (HTV-1) maintain viremia below the limit of detection without antiretroviral treatment. Virus-specific cytotoxic CD8+ T lymphocytes are believed to play a crucial role in viral containment ...
C. J. Brumme, F. Pereyra, Harrigan, P. R., Brumme, Z. L., John, M., B. D. Walker, Brockman, M. A., P. R. Harrigan, T. Miura, Miura, T., Block, B. L., Trocha, A., M. A. Brockman, M. John, A. Trocha, Z. L. Brumme, S. Mallal, Pereyra, F., Walker, B. D., B. L. Block, Brumme, C. J., Mallal, S.   +21 more
core   +1 more source

Characterization of HIV-1 gag and nef in Cameroon: further evidence of extreme diversity at the origin of the HIV-1 group M epidemic

Virology Journal, 2013
Background Cameroon, in west central Africa, has an extraordinary degree of HIV diversity, presenting a major challenge for the development of an effective HIV vaccine. Given the continuing need to closely monitor the emergence of new HIV variants in the
Tongo Marcel, Martin Darren P, Zembe Lycias, Mpoudi-Ngole Eitel, Williamson Carolyn, Burgers Wendy A   +5 more
doaj   +1 more source

Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus

Advanced Science, EarlyView.
This study uncovers a conserved PIP2‐dependent secretory pathway of orbivirus NS3 that induces vascular leakage. Pharmacological disruption of PIP2‐NS3 interaction significantly reduces viral pathogenicity and provides protective efficacy in murine models, establishing PIP2‐mediated NS3 secretion as both a key virulence determinant and a promising ...
Junyong Guan, Dong Zhou, Ran Shao, Xing Liu, Jin Peng, Yingran Huang, Shuhui Qi, Cankun Xi, Menghang Wang, Bin Sun, Yinglin Qi, Xin Yin   +11 more
wiley   +1 more source

Properties of a chimeric simian-human immunodeficiency virus expressing an hybrid HIV-1 Nef/SIVmac Nef protein

, 2002
International audienceThe nef genes of human and simian immunodeficiency viruses code for a membrane associated protein critical for AIDS development. SIVmac Nef presents C-terminal a 27 amino acid extension absent of HIV-1 Nef. To estimate the influence
Beyer, C., Gut, J., Cluet, D., Cecile, A., Bertsch, C., Gloeckler, L., Aubertin, A.   +6 more
core   +1 more source