tRNA engineering strategies for genetic code expansion [PDF]
Frontiers in GeneticsThe advancement of genetic code expansion (GCE) technology is attributed to the establishment of specific aminoacyl-tRNA synthetase/tRNA pairs. While earlier improvements mainly focused on aminoacyl-tRNA synthetases, recent studies have highlighted the ...
YouJin Kim +3 more
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Genetic Code Expansion of Vibrio natriegens [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Escherichia coli has been considered as the most used model bacteria in the majority of studies for several decades. However, a new, faster chassis for synthetic biology is emerging in the form of the fast-growing gram-negative bacterium Vibrio ...
Eden Ozer, Lital Alfonta
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Use of non‐canonical amino acids in genetic code expansion‐based therapeutics: Effects on mouse gut microbiota [PDF]
Microbial Biotechnology, 2023 Vaccines and cell therapeutics based on genetic code expansion are emerging. A crucial step in these therapeutic technologies is the oral administration of non‐canonical amino acids (ncAAs) to control pathogen growth and therapeutic protein levels in ...
Manyu Liang +7 more
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Studying Acetylation of Aconitase Isozymes by Genetic Code Expansion [PDF]
Frontiers in Chemistry, 2022 Aconitase catalyzes the second reaction of the tricarboxylic acid cycle, the reversible conversion of citrate and isocitrate. Escherichia coli has two isoforms of aconitase, AcnA and AcnB. Acetylomic studies have identified acetylation at multiple lysine
Jessica Araujo +5 more
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Improving the Efficiency and Orthogonality of Genetic Code Expansion [PDF]
BioDesign Research, 2022 The site-specific incorporation of the noncanonical amino acid (ncAA) into proteins via genetic code expansion (GCE) has enabled the development of new and powerful ways to learn, regulate, and evolve biological functions in vivo.
Xian Fu, Yijian Huang, Yue Shen
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Applications of genetic code expansion and photosensitive UAAs in studying membrane proteins [PDF]
Open Life Sciences, 2023 Membrane proteins are the targets for most drugs and play essential roles in many life activities in organisms. In recent years, unnatural amino acids (UAAs) encoded by genetic code expansion (GCE) technology have been widely used, which endow proteins ...
Zhao Shu, Liu Dong
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Suppressor tRNAs at the interface of genetic code expansion and medicine [PDF]
Frontiers in GeneticsSuppressor transfer RNAs (sup-tRNAs) are receiving renewed attention for their promising therapeutic properties in treating genetic diseases caused by nonsense mutations.
Aya Awawdeh +2 more
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Remodeling the cellular stress response for enhanced genetic code expansion in mammalian cells [PDF]
Nature Communications, 2023 Genetic code expansion (GCE) reprograms the translational machinery to site-specifically incorporate noncanonical amino acids (ncAAs) into a selected protein.
Mikhail E. Sushkin +2 more
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Genetic code expansion reveals site-specific lactylation in living cells reshapes protein functions [PDF]
Nature CommunicationsProtein lactylation is an emerging field. To advance the exploration of its biological functions, here we develop a comprehensive workflow that integrates proteomics to identify lactylated sites, genetic code expansion (GCE) for the expression of site ...
Chang Shao +15 more
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A robust platform streamlining aromatic noncanonical amino acid biosynthesis and genetic code expansion in Escherichia coli [PDF]
Nature CommunicationsGenetic code expansion (GCE) has significantly enhanced the diversity of proteins in the biological world, leading to a wide range of applications.
Jingxuan Zhang +8 more
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