Results 11 to 20 of about 273,398 (272)
Therapeutic applications of genetic code expansion [PDF]
Synthetic and Systems Biotechnology, 2018 In nature, a limited, conservative set of amino acids are utilized to synthesize proteins. Genetic code expansion technique reassigns codons and incorporates noncanonical amino acids (ncAAs) through orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pairs.
Yujia Huang, Tao Liu
doaj +4 more sources
Genetic Code Expansion Approaches to Decipher the Ubiquitin Code. [PDF]
Chem RevISSN:0009 ...
Wanka V, Fottner M, Cigler M, Lang K.
europepmc +5 more sources
Switchable genome editing via genetic code expansion [PDF]
Scientific Reports, 2018 Multiple applications of genome editing by CRISPR-Cas9 necessitate stringent regulation and Cas9 variants have accordingly been generated whose activity responds to small ligands, temperature or light.
Asami, Maki +5 more
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Using Genetic Code Expansion for Protein Biochemical Studies [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the
Christina Z. Chung +3 more
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The Role of Orthogonality in Genetic Code Expansion [PDF]
Life, 2019 The genetic code defines how information in the genome is translated into protein. Aside from a handful of isolated exceptions, this code is universal. Researchers have developed techniques to artificially expand the genetic code, repurposing codons and ...
Pol Arranz-Gibert +2 more
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Establishing the fundamental rules for genetic code expansion. [PDF]
Nat Chem, 2023 Genetic code expansion beyond α-amino acids is a major challenge, in which stitching non-natural building blocks within the ribosome is a critical barrier. Now, the molecular determinants for the efficient incorporation of non-natural amino acids within the ribosome have been unlocked, accelerating ribosomal synthesis.
Sinha S, Ahsan M, Palermo G.
europepmc +5 more sources
Genetic Code Expansion in Animals [PDF]
ACS Chemical Biology, 2018 Expanding the genetic code to enable the incorporation of unnatural amino acids into proteins in biological systems provides a powerful tool for studying protein structure and function. While this technology has been mostly developed and applied in bacterial and mammalian cells, it recently expanded into animals, including worms, fruit flies, zebrafish,
Wes Brown, Jihe Liu, Alexander Deiters
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Next-generation genetic code expansion [PDF]
Current Opinion in Chemical Biology, 2018 Engineering of the translation apparatus has permitted the site-specific incorporation of nonstandard amino acids (nsAAs) into proteins, thereby expanding the genetic code of organisms. Conventional approaches have focused on porting tRNAs and aminoacyl-tRNA synthetases (aaRS) from archaea into bacterial and eukaryotic systems where they have been ...
Pol, Arranz-Gibert +2 more
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Frontiers in Molecular Biosciences, 2023 Post-translational modifications (PTMs) can occur on almost all amino acids in eukaryotes as a key mechanism for regulating protein function. The ability to study the role of these modifications in various biological processes requires techniques to ...
Pearl Morosky +8 more
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Engineering Pyrrolysine Systems for Genetic Code Expansion and Reprogramming. [PDF]
Chem RevDunkelmann DL, Chin JW.
europepmc +2 more sources