Genetic Code Expansion in Pseudomonas putida KT2440. [PDF]
ACS Synth Biol, 2022 Pseudomonas putida KT2440 is an emerging microbial chassis for biobased chemical production from renewable feedstocks and environmental bioremediation. However, tools for studying, engineering, and modulating protein complexes and biosynthetic enzymes in this organism are largely underdeveloped. Genetic code expansion for the incorporation of unnatural
He X +5 more
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Configuration of Stable Evolutionary Strategy of Homo Sapiens and Evolutionary Risks of Technological Civilization (the Conceptual Model Essay) [PDF]
, 2014 Stable evolutionary strategy of Homo sapiens (SESH) is built in accordance with the modular and hierarchical principle and consists of the same type of self-replicating elements, i.e. is a system of systems.
Cheshko, Valentin T. +2 more
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Reading and Writing the Ubiquitin Code Using Genetic Code Expansion. [PDF]
ChembiochemAbstractDeciphering ubiquitin proteoform signaling and its role in disease has been a long‐standing challenge in the field. The effects of ubiquitin modifications, its relation to ubiquitin‐related machineries, and its signaling output has been particularly limited by its reconstitution and means of characterization.
Patel RS, Pannala NM, Das C.
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Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion
Croatica Chemica Acta, 2016 Genetic code expansion (GCE) has become a central topic of synthetic biology. GCE relies on engineered aminoacyl‐tRNA synthetases (aaRSs) and a cognate tRNA species to allow codon reassignment by co-translational insertion of non-canonical amino acids ...
Ana Crnković +3 more
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Frontiers in Microbiology, 2020 Due to the rapid emergence of multi-resistant bacterial strains in recent decades, the commercially available effective antibiotics are becoming increasingly limited.
Hamid Reza Karbalaei-Heidari +3 more
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Directed evolution of rRNA improves translation kinetics and recombinant protein yield
Nature Communications, 2021 Ribosome kinetics are rate-limiting for protein synthesis. Here the authors evolve diverse 16S rRNAs for enhanced protein synthesis rates and genetic code expansion efficiencies in vivo.
Fan Liu +4 more
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Applications of genetic code expansion technology in eukaryotes. [PDF]
Protein Cell, 2023 Abstract Unnatural amino acids (UAAs) have gained significant attention in protein engineering and drug development owing to their ability to introduce new chemical functionalities to proteins. In eukaryotes, genetic code expansion (GCE) enables the incorporation of UAAs and facilitates posttranscriptional modification (PTM), which is ...
Guo QR, Cao YJ.
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Recent Development of Genetic Code Expansion for Posttranslational Modification Studies
Molecules, 2018 Nowadays advanced mass spectrometry techniques make the identification of protein posttranslational modifications (PTMs) much easier than ever before. A series of proteomic studies have demonstrated that large numbers of proteins in cells are modified by
Hao Chen +4 more
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Measuring the tolerance of the genetic code to altered codon size
eLife, 2022 Translation using four-base codons occurs in both natural and synthetic systems. What constraints contributed to the universal adoption of a triplet codon, rather than quadruplet codon, genetic code?
Erika Alden DeBenedictis +2 more
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Population genomics of the Asian tiger mosquito, Aedes albopictus. Insights into the recent worldwide invasion [PDF]
, 2017 Aedes albopictus, the “Asian tiger mosquito,” is an aggressive biting mosquito native to Asia that has colonized all continents except Antarctica during the last ~30–40 years. The species is of great public health
Aguirre-Obando +86 more
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