Results 41 to 50 of about 273,398 (272)

Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights

Nature Communications, 2021
B. subtilis is valuable both as a model for cell biology and as an industrial organism. Here the authors use genetic code expansion to enable functional tools for exploring cell division dynamics.
Devon A. Stork, Georgia R. Squyres, Erkin Kuru, Katarzyna A. Gromek, Jonathan Rittichier, Aditya Jog, Briana M. Burton, George M. Church, Ethan C. Garner, Aditya M. Kunjapur   +9 more
doaj   +1 more source

Phospho-Form Specific Substrates of Protein Kinase B (AKT1)

Frontiers in Bioengineering and Biotechnology, 2021
Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and ...
McShane McKenna, Nileeka Balasuriya, Shanshan Zhong, Shawn Shun-Cheng Li, Patrick O'Donoghue, Patrick O'Donoghue   +5 more
doaj   +1 more source

Genetic Code Expansion: Recent Developments and Emerging Applications. [PDF]

Chem Rev
The concept of genetic code expansion (GCE) has revolutionized the field of chemical and synthetic biology, enabling the site-specific incorporation of noncanonical amino acids (ncAAs) into proteins, thus opening new avenues in research and applications across biology and medicine.
Huang Y, Zhang P, Wang H, Chen Y, Liu T, Luo X.   +5 more
europepmc   +3 more sources

Genetic code expansion for multiprotein complex engineering [PDF]

Nature Methods, 2016
We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins ...
Christine Koehler, Paul F Sauter, Mirella Wawryszyn, Gemma Estrada Girona, Kapil Gupta, Jonathan J M Landry, Markus Hsi-Yang Fritz, Ksenija Radic, Jan-Erik Hoffmann, Zhuo A Chen, Juan Zou, Piau Siong Tan, Bence Galik, Sini Junttila, Peggy Stolt-Bergner, Giancarlo Pruneri, Attila Gyenesei, Carsten Schultz, Moritz Bosse Biskup, Hueseyin Besir, Vladimir Benes, Juri Rappsilber, Martin Jechlinger, Jan O Korbel, Imre Berger, Stefan Braese, Edward A Lemke   +26 more
openaire   +5 more sources

The Application of Cell-Free Protein Synthesis in Genetic Code Expansion for Post-translational Modifications

Frontiers in Pharmacology, 2019
The translation system is a sophisticated machinery that synthesizes proteins from 20 canonical amino acids. Recently, the repertoire of such composition has been expanded by the introduction of non-canonical amino acids (ncAAs) with the genetic code ...
Sumana Venkat, Hao Chen, Qinglei Gan, Chenguang Fan, Chenguang Fan   +4 more
doaj   +1 more source

Recent Synthetic Biology Approaches for Temperature- and Light-Controlled Gene Expression in Bacterial Hosts

Molecules, 2022
The expression of genes of interest (GOI) can be initiated by providing external stimuli such as temperature shifts and light irradiation. The application of thermal or light stimuli triggers structural changes in stimuli-sensitive biomolecules within ...
Jongdoo Choi, Jiyeun Ahn, Jieun Bae, Minseob Koh   +3 more
doaj   +1 more source

Admixture in the Hispanics of the San Luis Valley, Colorado, and its implications for complex trait gene mapping. [PDF]

, 2004
Hispanic populations are a valuable resource that can and should facilitate the identification of complex trait genes by means of admixture mapping (AM).
Bonilla, C, Dios, S, Ferrell, RE, Hamman, RF, Hoggart, CL, Marshall, JA, McKeigue, PM, Parra, EJ, Pfaff, CL, Shriver, MD   +9 more
core   +1 more source

Metalloprotein design using genetic code expansion

Chem. Soc. Rev., 2014
Genetic code expansion has become an essential new tool for designing functional small protein models for complex metalloenzymes.
Hu, Cheng, Chan, Sunney I., Sawyer, Elizabeth B., Yu, Yang, Wang, Jiangyun   +4 more
openaire   +3 more sources

Genetic code expansion in the mouse brain [PDF]

Nature Chemical Biology, 2016
Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNA(Pyl)CUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms.
Russell J Ernst, Toke P Krogager, Elizabeth S Maywood, Roberto Zanchi, Václav Beránek, Thomas S Elliott, Nicholas P Barry, Michael H Hastings, Jason W Chin   +8 more
openaire   +2 more sources

Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines

Insects, 2021
Inducible gene expression systems are important tools for studying gene function and to control protein synthesis. With the completion of the detailed map of the silkworm (Bombyx mori) genome, the study of Bombyx mori has entered the post-genome era ...
Wei Lu, Ruolin Wang, Pan Wang, Sanyuan Ma, Qingyou Xia   +4 more
doaj   +1 more source