Results 91 to 100 of about 58,106,402 (334)

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Genotyping-by-sequencing (GBS): a novel, efficient and cost-effective genotyping method for cattle using next-generation sequencing. [PDF]

PLoS ONE, 2013
High-throughput genotyping methods have increased the analytical power to study complex traits but high cost has remained a barrier for large scale use in animal improvement. We have adapted genotyping-by-sequencing (GBS) used in plants for genotyping 47
Marcos De Donato, Sunday O Peters, Sharon E Mitchell, Tanveer Hussain, Ikhide G Imumorin   +4 more
doaj   +1 more source

Potential of Low‐Coverage Genotyping‐by‐Sequencing and Imputation for Cost‐Effective Genomic Selection in Biparental Segregating Populations

, 2017
Genotyping-by-sequencing (GBS) is an alternative genotyping method to singlenucleotide polymorphism (SNP) arrays that has received considerable attention in the plant breeding community.
G. Gorjanc, Jean-François Dumasy, S. Gonen, R. C. Gaynor, Roberto Antolín, John M. Hickey   +5 more
semanticscholar   +1 more source

SNP types identified in this study through genotyping-by-sequencing.

, 2018
SNP types identified in this study through genotyping-by-sequencing.
Todd C. Mockler (110557), Erik R. Rowley (516360), Roberto Botta (5048093), Shawn A. Mehlenbacher (4040144), Ezio Portis (86400), Alberto Acquadro (3129291), Daniela Torello Marinoni (5048096), Nadia Valentini (5048099), Chiara Beltramo (5048102)   +8 more
core   +1 more source

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma

Molecular Oncology, EarlyView.
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara, Kosuke Murakami, Shiro Takamatsu, Akiko Kanto, Seiichi Mori, Tomoyuki Otani, Kazuko Sakai, Yasushi Kotani, Kazuto Nishio, Kaoru Abiko, Noriomi Matsumura   +10 more
wiley   +1 more source

Optimized double-digest genotyping by sequencing (ddGBS) method with high-density SNP markers and high genotyping accuracy for chickens

PLoS ONE, 2017
High-density single nucleotide polymorphism (SNP) markers are crucial to improve the resolution and accuracy of genome-wide association study (GWAS) and genomic selection (GS).
Yu-Zhe Wang, Xuemin Cao, Yi-Qiang Zhao, J. Fei, Xiao-Xiang Hu, Ning Li   +5 more
semanticscholar   +1 more source

LASER server: ancestry tracing with genotypes or sequence reads [PDF]

Bioinformatics, 2017
Abstract Summary To enable direct comparison of ancestry background in different studies, we developed LASER to estimate individual ancestry by placing either sezquenced or genotyped samples in a common ancestry space, regardless of the sequencing strategy or genotyping array used to characterize each
Daniel Taliun, Sonia P. Chothani, Sebastian Schönherr, Lukas Forer, Michael Boehnke, Gonçalo R. Abecasis, Chaolong Wang   +6 more
openaire   +3 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Genome-wide association studies of seven agronomic traits under two sowing conditions in bread wheat

BMC Plant Biology, 2019
Background Wheat is a cool seasoned crop requiring low temperature during grain filling duration and therefore increased temperature causes significant yield reduction.
Muhammad Jamil, Aamir Ali, Alvina Gul, Abdul Ghafoor, Abdul Aziz Napar, Amir M. H. Ibrahim, Naima Huma Naveed, Nasim Ahmad Yasin, Abdul Mujeeb-Kazi   +8 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source