Results 111 to 120 of about 58,106,402 (334)
BMC Genomics, 2016 Knowledge on population structure and genetic diversity in vegetable crops is essential for association mapping studies and genomic selection. Genotyping by sequencing (GBS) represents an innovative method for large scale SNP detection and genotyping of ...
F. Taranto +4 more
semanticscholar +1 more source
Bioinformatic analysis of genotype by sequencing (GBS) data with NGSEP
BMC Genomics, 2016 Therecent development and availability of different genotype by sequencing (GBS) protocols provided a cost-effective approach to perform high-resolution genomic analysis of entire populations in different species. The central component of all these protocols is the digestion of the initial DNA with known restriction enzymes, to generate sequencing ...
Perea, Claudia +7 more
openaire +3 more sources
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Genotyping-by-Sequencing: efficiency and reproducibility checks
, 2015 Genotyping-by-Sequencing (GBS) has the potential to be a cost effective, reproducible and high-throughput SNP genotyping method. We have been investigating GBS in a number of livestock species, with an emphasis on Sheep.
Rudiger Brauning (47247) +4 more
core
CTAB DNA Extraction and Genotyping-by-Sequencing to Map Meiotic Crossovers in Plants
, 2022 Reciprocal DNA crossovers between chromosomes form new allelic combinations and contribute to the formation of novel genetic diversity. Crossovers are formed during meiosis of germ cells and these recombination events have influenced plant genome ...
Lambing, C. +4 more
core +1 more source
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
PLoS ONE, 2016 Next-generation sequencing (NGS) has revolutionized plant and animal research in many ways including new methods of high throughput genotyping. Genotyping-by-sequencing (GBS) has been demonstrated to be a robust and cost-effective genotyping method ...
D. Torkamaneh, J. Laroche, F. Belzile
semanticscholar +1 more source
Genotyping-by-Sequencing - Development in livestock
, 2015 Recent advances in next generation sequencing technology have increased the output/cost to a level that where genotyping-by-sequencing (GBS) in livestock and plant crops as well as aquaculture species can be considered. GBS has the potential to be a cost
Rudiger Brauning (47247) +4 more
core
FEBS Open Bio, EarlyView.Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata +3 more
wiley +1 more source
Nested Association Mapping of Stem Rust Resistance in Wheat Using Genotyping by Sequencing
PLoS ONE, 2016 We combined the recently developed genotyping by sequencing (GBS) method with joint mapping (also known as nested association mapping) to dissect and understand the genetic architecture controlling stem rust resistance in wheat (Triticum aestivum).
P. Bajgain +6 more
semanticscholar +1 more source