Genotyping of pooled microsatellite markers by combinatorial optimization techniques
AbstractAn important everyday task for geneticists and molecular biologists is that of isolating and analyzing some particular DNA regions (markers), each drawn from a limited and known set of possible values (alleles). This procedure is called genotyping and is based on DNA amplification and size separation.
LANCIA, Giuseppe, PERLIN M.
openaire +3 more sources
High Frequency of Chlamydia trachomatis Mixed Infections Detected by Microarray Assay in South American Samples. [PDF]
Chlamydia trachomatis is one of the most common sexually transmitted infections worldwide. Based on sequence variation in the ompA gene encoding the major outer membrane protein, the genotyping scheme distinguishes 17 recognized genotypes, i.e. A, B, Ba,
Lucía Gallo Vaulet+7 more
doaj +1 more source
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population [PDF]
Single nucleotide polymorphisms (SNPs) which represent the most widespread source of sequence variation in genomes, are becoming a routine application in several fields such as forensics, ecology and conservation genetics.
Fabbri Elena+7 more
doaj +1 more source
The objective of this study was to standardize a method for extracting high-quality DNA from samples of coagulated blood. Forty-eight samples of human coagulated blood were used for DNA extraction by means of the EZ-DNA commercial kit (Biological ...
D. Cardozo+7 more
semanticscholar +1 more source
SELECTION OF BARLEY (HORDEUM VULGARE) GENOTYPES BY GYT (GENOTYPE × YIELD × TRAIT) BIPLOT TECHNIQUE AND ITS COMPARISON WITH GT (GENOTYPE × TRAIT) [PDF]
The selection of genotypes based on multiple traits and multiple environments is very important to improve stable varieties for breeding programs. Since climatic conditions are very variable and unpredictable, they are very effective in the selection of genotypes based on multiple traits in multiple environmental conditions. Thus, the genotype by yield*
Karahan, T., Akgun, İlknur
openaire +2 more sources
Crop Yield Prediction Using Deep Neural Networks [PDF]
Crop yield is a highly complex trait determined by multiple factors such as genotype, environment, and their interactions. Accurate yield prediction requires fundamental understanding of the functional relationship between yield and these interactive factors, and to reveal such relationship requires both comprehensive datasets and powerful algorithms ...
arxiv +1 more source
A genotyping protocol for multiple tissue types from the polyploid tree species Sequoia sempervirens (Cupressaceae). [PDF]
Premise of the studyIdentifying clonal lineages in asexually reproducing plants using microsatellite markers is complicated by the possibility of nonidentical genotypes from the same clonal lineage due to somatic mutations, null alleles, and scoring ...
Dodd, Richard S+2 more
core +2 more sources
Genomic relatedness within five common Finnish Campylobacter jejuni pulsed-field gel electrophoresis genotypes studied by amplified fragment length polymorphism analysis, ribotyping and serotyping [PDF]
Thirty-five Finnish Campylobacter jejuni strains with five SmaI/SacII pulsed-field gel electrophoresis (PFGE) genotypes selected among human and chicken isolates from 1997 and 1998 were used for comparison of their PFGE patterns, amplified fragment ...
Duim, B.+4 more
core +3 more sources
The development of a novel SNP genotyping assay to differentiate cacao clones [PDF]
In this study, a double-mismatch allele-specific (DMAS) qPCR SNP genotyping method has been designed, tested and validated specifically for cacao, using 65 well annotated international cacao reference accessions retrieved from the Center for Forestry ...
Coppieters, Frauke+6 more
core +1 more source
Genetic variability of blood groups in southern Brazil
We evaluated genetic variability among the blood groups Kell (c.578C > T and c.1790T > C), Kidd (c.838A > G), Duffy (c.125A > G, c.265C > T and c.1-67T > C), Diego (c.2561C > T), MNS (c.143T > C) and Rh (c.676G > C) in Rio Grande do Sul in southern ...
Gabriela Waskow+6 more
doaj +1 more source