Results 71 to 80 of about 11,640,365 (320)

Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications

PLoS ONE, 2018
Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered by poor DNA quality and purity, particularly in challenging plant species, rich in secondary metabolites.
P. Inglis, M. Pappas, L. V. Resende, D. Grattapaglia   +3 more
semanticscholar   +1 more source

Phenotypic and genotypic characterization of single circulating tumor cells in the follow‐up of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Single circulating tumor cells (sCTCs) from high‐grade serous ovarian cancer patients were enriched, imaged, and genomically profiled using WGA and NGS at different time points during treatment. sCTCs revealed enrichment of alterations in Chromosomes 2, 7, and 12 as well as persistent or emerging oncogenic CNAs, supporting sCTC identity.
Carolin Salmon, Rui P. L. Neves, Nikolas H. Stoecklein, Sven‐Thorsten Liffers, Jens Siveke, Jan D. Kuhlmann, Pauline Wimberger, Paul Buderath, Rainer Kimmig, Sabine Kasimir‐Bauer   +9 more
wiley   +1 more source

Six novel O genotypes from Shiga toxin-producing Escherichia coli

Frontiers in Microbiology, 2016
Serotyping is one of the typing techniques used to classify strains within the same species. O-serogroup diversification shows a strong association with the genetic diversity of O-antigen biosynthesis genes.
Atsushi eIguchi, Sunao eIyoda, Kazuko eSeto, Hironobu eNishii, Makoto eOhnishi, Hirohisa eMekata, Hirohisa eMekata, Yoshitoshi eOgura, Tetsuya eHayashi   +8 more
doaj   +1 more source

Individual Identification of Cartilage by Direct Amplification in Mass Disasters [PDF]

Fayixue Zazhi, 2017
Objective To explore the effectiveness of direct amplification for the STR analysis of cartilage, and to accelerate the effectiveness of disaster victim identification.
WANG CHUAN-HAI, XU CHENG, LI XIANG-QIN, ET AL.
doaj   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Dealing with AFLP genotyping errors to reveal genetic structure in Plukenetia volubilis (Euphorbiaceae) in the Peruvian Amazon.

PLoS ONE, 2017
An analysis of the population structure and genetic diversity for any organism often depends on one or more molecular marker techniques. Nonetheless, these techniques are not absolutely reliable because of various sources of errors arising during the ...
Jakub Vašek, Petra Hlásná Čepková, Iva Viehmannová, Martin Ocelák, Danter Cachique Huansi, Pavel Vejl   +5 more
doaj   +1 more source

Genome-wide SNP typing of ancient DNA: Determination of hair and eye color of Bronze Age humans from their skeletal remains. [PDF]

, 2020
Objective A genome-wide high-throughput single nucleotide polymorphism (SNP) typing method was tested with respect of the applicability to ancient and degraded DNA. The results were compared to mini-sequencing data achieved through single base extension (
Dörk, T., Hummel, S., Klintschar, M., Krause, I., Schmidt, N., Schücker, K.   +5 more
core   +2 more sources

High throughput SNP discovery and genotyping in hexaploid wheat

PLoS ONE, 2018
Because of their abundance and their amenability to high-throughput genotyping techniques, Single Nucleotide Polymorphisms (SNPs) are powerful tools for efficient genetics and genomics studies, including characterization of genetic resources, genome-wide
Hélène Rimbert, Benoît Darrier, Julien Navarro, Jonathan Kitt, F. Choulet, M. Leveugle, Jorge Duarte, N. Rivière, K. Eversole, J. Le Gouis, Alessandro Davassi, F. Balfourier, M. Le Paslier, A. Bérard, D. Brunel, C. Feuillet, C. Poncet, P. Sourdille, E. Paux   +18 more
semanticscholar   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

GAWMerge expands GWAS sample size and diversity by combining array-based genotyping and whole-genome sequencing

Communications Biology, 2022
GAWMerge is a computational tool that allows users to integrate SNP genotyping data from array techniques or whole-genome sequencing, providing a feasible method to leverage existing cohorts to increase sample size in genetic studies.
Ravi Mathur, Fang Fang, Nathan Gaddis, Dana B. Hancock, Michael H. Cho, John E. Hokanson, Laura J. Bierut, Sharon M. Lutz, Kendra Young, Albert V. Smith, NHLBI Trans-Omics for Precision Medicine (TOPMed) Consortium, Edwin K. Silverman, Grier P. Page, Eric O. Johnson   +13 more
doaj   +1 more source