Results 81 to 90 of about 83,652 (291)

Genotyping Techniques for Determining the Diversity of Microorganisms

, 2012
Typing of microbial pathogens, or identifying bacteria at the strain level, is particularly important for diagnosis, treatment, and epidemiological surveillance of bacterial infections. This is especially the case for bacteria exhibiting high levels of antibiotic resistance or virulence, and those involved in nosocomial or pandemic infections.
Katarzyna Wolska, Piotr Szwe
openaire   +2 more sources

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

A novel technique for NOD2/CARD15 genotyping using PCR-SSP

Journal of Immunological Methods, 2007
The Nucleotide-binding Oligomerisation Domain (NOD) 2 protein is encoded by the Caspase Recruitment Domain (CARD) 15 gene and has a critical role in innate immunity. Recent studies have implicated Single Nucleotide Polymorphisms (SNPs) of the NOD2/CARD15 gene with the onset of several Inflammatory Bowel Disorders (Crohn's Disease, Blau syndrome) and ...
Mayor, N, Shaw, B, Keshav, S, Madrigal, J, Marsh, S   +4 more
openaire   +3 more sources

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Isotachophoresis preconcentration integrated microfluidic chip for highly sensitive genotyping of the hepatitis B virus

, 2006
The genotyping of hepatitis B virus (HBV) has become recently a valuable tool not only for epidemiological reasons but also for the clinical practice.
Qin, Jianhua, 林炳承, Shi, Ming, Huang, Huaiqing, Zhou, Xiaomian, Liu, Dayu, Long, Zhicheng, Lin, Bingcheng   +7 more
core   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Smart breeding driven by advances in sequencing technology

Modern Agriculture, 2023
Crop breeding has undergone numerous advancements throughout history with the next revolution being uncertain. This review provides an overview of breeding techniques from traditional selective breeding and crossbreeding to modern breeding based on ...
Chenji Zhang, Sirong Jiang, Yangyang Tian, Xiaorui Dong, Jianjia Xiao, Yanjie Lu, Tiyun Liang, Hongmei Zhou, Dabin Xu, Han Zhang, Ming Luo, Zhiqiang Xia   +11 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Preservation of a rare bog pine genotypes using micropropagation techniques

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2014
Bog pine (Pinus uncinata DC. subsp. uliginosa (Neumann) Businský) is a subendemic species and appears to be one of the most endangered tree species of the Czech Republic. Its rare populations are at the present time greatly endangered namely by spontaneous hybridization with the Scots pine (Pinus sylvestris L.).
Hana Vejsadová, Helena Vlašínová, Ladislav Havel   +2 more
openaire   +2 more sources

Priors for genotyping polyploids

, 2020
Motivation: Empirical Bayes techniques to genotype polyploid organisms usually either (i) assume technical artifacts are known a priori or (ii) estimate technical artifacts simultaneously with the prior genotype distribution.
Luís Felipe Ventorim Ferrão (16785594), David Gerard (14620685)   +1 more
core   +1 more source