Results 61 to 70 of about 153,555 (331)

A novel technique for NOD2/CARD15 genotyping using PCR-SSP

Journal of Immunological Methods, 2007
The Nucleotide-binding Oligomerisation Domain (NOD) 2 protein is encoded by the Caspase Recruitment Domain (CARD) 15 gene and has a critical role in innate immunity. Recent studies have implicated Single Nucleotide Polymorphisms (SNPs) of the NOD2/CARD15 gene with the onset of several Inflammatory Bowel Disorders (Crohn's Disease, Blau syndrome) and ...
Neema P. Mayor, Steven G.E. Marsh, Steven G.E. Marsh, J. Alejandro Madrigal, J. Alejandro Madrigal, Satish Keshav, Bronwen E. Shaw, Bronwen E. Shaw   +7 more
openaire   +4 more sources

Evaluation of carbapenem resistance using phenotypic and genotypic techniques in Enterobacteriaceae isolates [PDF]

Annals of Clinical Microbiology and Antimicrobials, 2015
Bacterial resistance to antibiotics is increasing worldwide. Antibiotic-resistant strains can lead to serious problems regarding treatment of infection. Carbapenem antibiotics are the final treatment option for infections caused by serious and life-threatening multidrug-resistant gram-negative bacteria.
Kazım Sahin, Ayse Tekin, Sule Ozdas, Demet Akin, Hande Yapislar, Aziz Ramazan Dilek, Emine Sonmez   +6 more
openaire   +4 more sources

RAD50 missense variants differentially affect the DNA damage response and mitotic progression

FEBS Letters, EarlyView.
RAD50 incorporates into the MRN complex and initiates the DNA damage response. Furthermore, RAD50 promotes mitotic progression. RAD50 missense variants capable of forming an MRN complex supported the DNA damage response and mitotic features to different extents in complementation experiments, indicating these functions are separable and might impact ...
Hanna Redeker, Swantje Kebel, Lea Völkening, Anna Vatselia, Louisa Weinhold, Girmay Asgedom, Axel Schambach, Detlev Schindler, Thilo Dörk, Kristine Bousset   +9 more
wiley   +1 more source

Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus [PDF]

Sensors, 2014
Objective: To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). Methods: The mercapto-modified B1; B2; C1; and C2-specific genotyping probes consisted of two probes for each HBV genotype that served as a double verification system. These probes were fixed on the surface of No. 1; 2; 3; and
Ai-Lin Liu, Jinyuan Chen, Qiang Yi, Qingqiong Chen, Shaohuang Weng, Fengqing Wang, Xinhua Lin, Qicai Liu, Jing Chen   +8 more
openaire   +3 more sources

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

Application of individualized therapy based on molecular classification in the treatment of medulloblastoma in children

Chinese Journal of Contemporary Neurology and Neurosurgery, 2020
Objective To explore the clinical efficacy of individualized therapy based on molecular classification in the treatment of medulloblastoma in children.
Wei SHI, Ping XUE, Jian⁃zhong YU, Rui ZHAO, Yi ZHANG, Hao LI   +5 more
doaj  

Comparative analysis of minor histocompatibility antigens genotyping methods

Онкогематология, 2016
The wide range of techniques could be employed to find mismatches in minor histocompatibility antigens between transplant recipients and their donors. In the current study we compared three genotyping methods based on polymerase chain reaction (PCR) for ...
A. S. Vdovin, A. M. Postovskaya, N. A. Bykova, D. S. Romaniuk, A. K. Alieva, P. R. Yefimova, S. A. Sheetikov, H. L. Julhakyan, G. A. Efimov   +8 more
doaj   +1 more source

Individual Identification of Cartilage by Direct Amplification in Mass Disasters [PDF]

Fayixue Zazhi, 2017
Objective To explore the effectiveness of direct amplification for the STR analysis of cartilage, and to accelerate the effectiveness of disaster victim identification.
WANG CHUAN-HAI, XU CHENG, LI XIANG-QIN, ET AL.
doaj   +1 more source

Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes

FEBS Letters, EarlyView.
The budding yeast Rad27 is a structure‐specific endonuclease. Here, the authors reveal that Rad27 is crucial for maintaining the stability of the ribosomal RNA gene (rDNA) region. Rad27 deficiency leads to the accumulation of Okazaki fragments and changes in rDNA copy number.
Tsugumi Yamaji, Yuko Katayama, Nanase Arata, Mariko Sasaki   +3 more
wiley   +1 more source

Six novel O genotypes from Shiga toxin-producing Escherichia coli

Frontiers in Microbiology, 2016
Serotyping is one of the typing techniques used to classify strains within the same species. O-serogroup diversification shows a strong association with the genetic diversity of O-antigen biosynthesis genes.
Atsushi eIguchi, Sunao eIyoda, Kazuko eSeto, Hironobu eNishii, Makoto eOhnishi, Hirohisa eMekata, Hirohisa eMekata, Yoshitoshi eOgura, Tetsuya eHayashi   +8 more
doaj   +1 more source