Results 71 to 80 of about 10,219,641 (384)

Detection rate for ESR1 mutations is higher in circulating‐tumor‐cell‐derived genomic DNA than in paired plasma cell‐free DNA samples as revealed by ddPCR

Molecular Oncology, EarlyView.
Analysis of ESR1 mutations in plasma cell‐free DNA (cfDNA) is highly important for the selection of treatment in patients with breast cancer. Using multiplex‐ddPCR and identical blood draws, we investigated whether circulating tumor cells (CTCs) and cfDNA provide similar or complementary information for ESR1 mutations.
Stavroula Smilkou, Aliki Ntzifa, Victoria Tserpeli, Ioanna Balgkouranidou, Alkistis Papatheodoridi, Evangelia Razis, Helena Linardou, Christos Papadimitriou, Amanda Psyrri, Flora Zagouri, Stylianos Kakolyris, Evi Lianidou   +11 more
wiley   +1 more source

Application of individualized therapy based on molecular classification in the treatment of medulloblastoma in children

Chinese Journal of Contemporary Neurology and Neurosurgery, 2020
Objective To explore the clinical efficacy of individualized therapy based on molecular classification in the treatment of medulloblastoma in children.
Wei SHI, Ping XUE, Jian⁃zhong YU, Rui ZHAO, Yi ZHANG, Hao LI   +5 more
doaj  

Dealing with AFLP genotyping errors to reveal genetic structure in Plukenetia volubilis (Euphorbiaceae) in the Peruvian Amazon.

PLoS ONE, 2017
An analysis of the population structure and genetic diversity for any organism often depends on one or more molecular marker techniques. Nonetheless, these techniques are not absolutely reliable because of various sources of errors arising during the ...
Jakub Vašek, Petra Hlásná Čepková, Iva Viehmannová, Martin Ocelák, Danter Cachique Huansi, Pavel Vejl   +5 more
doaj   +1 more source

Genetic mapping of legume orthologs reveals high conservation of synteny between lentil species and the sequenced genomes of Medicago and chickpea. [PDF]

, 2014
Lentil (Lens culinaris Medik.) is a global food crop with increasing importance for food security in south Asia and other regions. Lens ervoides, a wild relative of cultivated lentil, is an important source of agronomic trait variation.
Albert eVandenberg, Andrew D. Farmer, Douglas R. Cook, Kirstin E. Bett, Neha eGujaria-Verma, Noelia eCarrasquilla-Garcia, R. Varma ePenmetsa, Sally L. Vail, Sally L. Vail, Sally L. Vail   +9 more
core   +3 more sources

A novel technique for NOD2/CARD15 genotyping using PCR-SSP

Journal of Immunological Methods, 2007
The Nucleotide-binding Oligomerisation Domain (NOD) 2 protein is encoded by the Caspase Recruitment Domain (CARD) 15 gene and has a critical role in innate immunity. Recent studies have implicated Single Nucleotide Polymorphisms (SNPs) of the NOD2/CARD15 gene with the onset of several Inflammatory Bowel Disorders (Crohn's Disease, Blau syndrome) and ...
Neema P. Mayor, Steven G.E. Marsh, Steven G.E. Marsh, J. Alejandro Madrigal, J. Alejandro Madrigal, Satish Keshav, Bronwen E. Shaw, Bronwen E. Shaw   +7 more
openaire   +4 more sources

Evaluation of carbapenem resistance using phenotypic and genotypic techniques in Enterobacteriaceae isolates [PDF]

Annals of Clinical Microbiology and Antimicrobials, 2015
Bacterial resistance to antibiotics is increasing worldwide. Antibiotic-resistant strains can lead to serious problems regarding treatment of infection. Carbapenem antibiotics are the final treatment option for infections caused by serious and life-threatening multidrug-resistant gram-negative bacteria.
Kazım Sahin, Ayse Tekin, Sule Ozdas, Demet Akin, Hande Yapislar, Aziz Ramazan Dilek, Emine Sonmez   +6 more
openaire   +4 more sources

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source

Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications

PLoS ONE, 2018
Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered by poor DNA quality and purity, particularly in challenging plant species, rich in secondary metabolites.
P. Inglis, M. Pappas, L. V. Resende, D. Grattapaglia   +3 more
semanticscholar   +1 more source

Automated SNP genotype clustering algorithm to improve data completeness in high-throughput SNP genotyping datasets from custom arrays [PDF]

, 2007
High-throughput SNP genotyping platforms use automated genotype calling algorithms to assign genotypes. While these algorithms work efficiently for individual platforms, they are not compatible with other platforms, and have individual biases that result
Edward M. Smith, Hardenbol, Hardenbol, Hua, Huentelman, Jack Littrell, Kissebah, Lamy, Liu, McPeek, Michael Olivier, Olivier, Rabbee, Smith, Sonnenberg, Xiao   +15 more
core   +2 more sources

Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus [PDF]

Sensors, 2014
Objective: To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). Methods: The mercapto-modified B1; B2; C1; and C2-specific genotyping probes consisted of two probes for each HBV genotype that served as a double verification system. These probes were fixed on the surface of No. 1; 2; 3; and
Ai-Lin Liu, Jinyuan Chen, Qiang Yi, Qingqiong Chen, Shaohuang Weng, Fengqing Wang, Xinhua Lin, Qicai Liu, Jing Chen   +8 more
openaire   +3 more sources