Germ Granules in Animal Oogenesis [PDF]
Journal of Developmental Biology, 2022 In eukaryotic cells, many macromolecules are organized as membraneless biomolecular condensates (or biocondensates). Liquid–liquid and liquid–solid phase transitions are the drivers of the condensation process.
Mikhail A. Dobrynin +2 more
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Plasticity of Drosophila germ granules during germ cell development. [PDF]
PLoS Biology, 2023 Compartmentalization of RNAs and proteins into membraneless structures called granules is a ubiquitous mechanism for organizing and regulating cohorts of RNAs.
Anna C Hakes, Elizabeth R Gavis
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C. elegans germ granules sculpt both germline and somatic RNAome [PDF]
Nature Communications, 2023 Germ granules are membrane-less organelles essential for small RNA biogenesis and germline development. Among the conserved properties of germ granules is their association with the nuclear membrane. Recent studies demonstrated that LOTUS domain proteins,
Ian F. Price +4 more
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Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila [PDF]
Cell Reports, 2023 Summary: RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation
Harrison A. Curnutte +8 more
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Germ Granules Allow Transmission of Small RNA-Based Parental Responses in the “Germ Plasm” [PDF]
iScience, 2020 Summary: In the recent decade small RNA-based inheritance has been implicated in a variety of transmitted physiological responses to the environment. In Caenorhabditis elegans, heritable small RNAs rely on RNA-dependent RNA polymerases, RNA-processing ...
Itamar Lev, Oded Rechavi
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Expansion microscopy reveals subdomains in C. elegans germ granules [PDF]
Life Science Alliance, 2023 Visualising C. elegans germ granules using the super resolution technique expansion microscopy against the backdrop of proteome staining reveals granule ultrastructure.
Kin M Suen +5 more
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Maternal dazap2 Regulates Germ Granules by Counteracting Dynein in Zebrafish Primordial Germ Cells
Cell Reports, 2015 Primordial germ cells (PGCs) are the stem cells of the germline. Generally, germline induction occurs via zygotic factors or the inheritance of maternal determinants called germ plasm (GP).
Meredyth M. Forbes +3 more
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Development, 2023 ABSTRACT A hallmark of all germ cells is the presence of germ granules: assemblies of proteins and RNA that lack a delineating membrane and are proposed to form via condensation. Germ granules across organisms share several conserved components, including factors required for germ cell fate determination and maintenance, and are thought ...
Thomas L, Putnam A, Folkmann A.
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Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans [PDF]
eLife, 2021 The germ line produces gametes that transmit genetic and epigenetic information to the next generation. Maintenance of germ cells and development of gametes require germ granules—well-conserved membraneless and RNA-rich organelles.
Ian F Price +4 more
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Nucleoporins shape germ granule architecture and balance small RNA silencing pathways [PDF]
Nature CommunicationsAnimals use small RNA pathways, such as PIWI-interacting RNA (piRNA) and small interfering RNA (siRNA), to silence harmful genetic elements. In Caenorhabditis elegans, piRNA pathway components are organized into sub-compartments within germ granules near
Kun Shi +7 more
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