Germ Granules Govern Small RNA Inheritance. [PDF]
Curr Biol, 2019 In C. elegans nematodes, components of liquid-like germ granules were shown to be required for transgenerational small RNA inheritance. Surprisingly, we show here that mutants with defective germ granules can nevertheless inherit potent small RNA-based silencing responses, but some of the mutants lose this ability after many generations of homozygosity.
Lev I +13 more
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Germ Granules Coordinate RNA-Based Epigenetic Inheritance Pathways. [PDF]
Dev Cell, 2019 AbstractGerm granules are biomolecular condensates that promote germ cell totipotency in most, if not all, animals. InC. elegans, MEG-3 and MEG-4 are two intrinsically disordered proteins that are redundantly required for the phase separations that drive germ granule assembly in germline blastomeres.
Dodson AE, Kennedy S.
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Drosophila germ granules are structured and contain homotypic mRNA clusters. [PDF]
Nat Commun, 2015 Abstract Germ granules, specialized ribonucleoprotein particles, are a hallmark of all germ cells. In Drosophila , an estimated 200 mRNAs are enriched in the germ plasm, and some of these have important, often conserved roles in germ cell formation, specification, survival ...
Trcek T +5 more
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Germ granules in Drosophila. [PDF]
Traffic, 2019 Abstract Germ granules are hallmarks of all germ cells. Early ultrastructural studies in Drosophila first described these membraneless granules in the oocyte and early embryo as filled with amorphous to fibrillar material mixed with RNA.
Trcek T, Lehmann R.
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Novel LOTUS-domain proteins are organizational hubs that recruit C. elegans Vasa to germ granules [PDF]
eLife, 2021 We describe MIP-1 and MIP-2, novel paralogous C. elegans germ granule components that interact with the intrinsically disordered MEG-3 protein. These proteins promote P granule condensation, form granules independently of MEG-3 in the postembryonic germ ...
Patricia Giselle Cipriani +13 more
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The piRNA protein Asz1 is essential for germ cell and gonad development in zebrafish and exhibits differential necessities in distinct types of germ granules. [PDF]
PLoS GeneticsGerm cells are essential for fertility, embryogenesis, and reproduction. Germline development requires distinct types of germ granules, which contains RNA-protein (RNP) complexes, including germ plasm in embryos, piRNA granules in gonadal germ cells, and
Adam Ahmad +5 more
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Correction: Expansion microscopy reveals subdomains in <i>C. elegans</i> germ granules. [PDF]
Life Sci Alliance, 2023 Correction to Materials and Methods.
Suen KM +5 more
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Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly development [PDF]
Nature CommunicationsDrosophila germ granules are enriched with mRNAs critical for development. Within them, mRNAs cluster through intermolecular interactions that may involve base pairing.
Siran Tian +5 more
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Convergent evolution of germ granule nucleators: A hypothesis
Stem Cell Research, 2017 Germ cells have been considered “the ultimate stem cell” because they alone, during normal development of sexually reproducing organisms, are able to give rise to all organismal cell types.
Arpita Kulkarni, Cassandra G. Extavour
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Germ granule compartments coordinate specialized small RNA production
Nature Communications, 2023 Germ granules are biomolecular condensates present in most animal germ cells. One function of germ granules is to help maintain germ cell totipotency by organizing mRNA regulatory machinery, including small RNA-based gene regulatory pathways.
Xiangyang Chen +14 more
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