Results 31 to 40 of about 32,670 (210)
Localisation of RNAs into the germ plasm of vitellogenic xenopus oocytes [PDF]
, 2013 We have studied the localisation of mRNAs in full-grown Xenopus laevis oocytes by injecting fluorescent RNAs, followed by confocal microscopy of the oocyte cortex.
B Heinrich +66 more
core +10 more sources
Germ granule-mediated RNA regulation in male germ cells [PDF]
Reproduction, 2018 Germ cells have exceptionally diverse transcriptomes. Furthermore, the progress of spermatogenesis is accompanied by dramatic changes in gene expression patterns, the most drastic of them being near-to-complete transcriptional silencing during the final steps of differentiation.
Tiina, Lehtiniemi, Noora, Kotaja
openaire +3 more sources
RNA Granules in Germ Cells [PDF]
Cold Spring Harbor Perspectives in Biology, 2011 "Germ granules" are cytoplasmic, nonmembrane-bound organelles unique to germline. Germ granules share components with the P bodies and stress granules of somatic cells, but also contain proteins and RNAs uniquely required for germ cell development. In this review, we focus on recent advances in our understanding of germ granule assembly, dynamics, and ...
Ekaterina, Voronina +3 more
openaire +2 more sources
Compartmentalized oskar degradation in the germ plasm safeguards germline development
eLife, 2020 Partitioning of mRNAs into ribonucleoprotein (RNP) granules supports diverse regulatory programs within the crowded cytoplasm. At least two types of RNP granules populate the germ plasm, a cytoplasmic domain at the posterior of the Drosophila oocyte and ...
Catherine E Eichler +3 more
doaj +1 more source
Bio-Protocol, 2022 Membraneless organelles, such as germ granules and stress granules, are liquid-like condensates formed by phase transition. Recently, we and others have adopted proximity-based labeling methods to determine the composition of these membraneless ...
Hannah Hertz, Ian Price, Wen Tang
doaj +1 more source
PLoS Genetics, 2023 Mammalian male germ cell differentiation relies on complex RNA biogenesis events, many of which occur in non-membrane bound organelles termed RNA germ cell granules that are rich in RNA binding proteins (RBPs).
Lauren G Chukrallah +3 more
doaj +1 more source
Nature Communications, 2022 Phase separated, membrane-less germ granules preserve fertility and cellular function in animal germ cells. Here the authors show that loss of germ granules impacts piRNA pathway fidelity in the recognition of self and non-self nucleic acids.
Wenjun Chen +7 more
doaj +1 more source
An Emerging Role for Post-translational Modifications in Regulating RNP Condensates in the Germ Line
Frontiers in Molecular Biosciences, 2021 RNA-binding proteins undergo regulated phase transitions in an array of cell types. The phase separation of RNA-binding proteins, and subsequent formation of RNP condensates or granules, occurs during physiological conditions and can also be induced by ...
Jennifer A. Schisa, Mohamed T. Elaswad
doaj +1 more source
Phase transitioned nuclear Oskar promotes cell division of Drosophila primordial germ cells
eLife, 2018 Germ granules are non-membranous ribonucleoprotein granules deemed the hubs for post-transcriptional gene regulation and functionally linked to germ cell fate across species.
Kathryn E Kistler +7 more
doaj +1 more source
Condensate cooperativity underlies transgenerational gene silencing
Cell Reports, 2023 Summary: Biomolecular condensates have been shown to interact in vivo, yet it is unclear whether these interactions are functionally meaningful. Here, we demonstrate that cooperativity between two distinct condensates—germ granules and P bodies—is ...
Zhenzhen Du +7 more
doaj +1 more source