Results 61 to 70 of about 98,730 (257)

Decoding Biomolecular Phase Separation: From Physiological Roles to Pathological Mechanisms and Therapeutic Biomaterials Design

Advanced Science, EarlyView.
This review systematically elaborates on the mechanisms underlying physiological regulation and pathological dysregulation of biomolecular phase separation. It also summarizes advances in phase‑separation‑targeted therapeutic strategies and the design of functional biomaterials inspired by this process.
Jiawei Zhu, Jingbo Dong, Qiufang Gong, Xuejiao Song, Zhifa Shen, Chao Liang   +5 more
wiley   +1 more source

A Postbiotic‐Inducing Ganoderma applanatum Polysaccharide Repairs the Intestinal Barrier via a Phocaeicola vulgatus ‐ Tridecylic Acid Axis

Advanced Science, EarlyView.
This study identifies GAP2, a bioactive polysaccharide fraction derived from Ganoderma applanatum, as a postbiotic inducer that repairs intestinal barrier damage in a gut microbiota‐dependent manner. GAP2 enriches Phocaeicola vulgatus, which degrades GAP2 and promotes tridecylic acid production, triggering Rho/CDC42 signaling and tight‐junction ...
Chuan Zhang, Miaoyu Li, Tiantian Li, Xiaojie Zang, Haiyang Shi, Yaru Liu, Chengcheng Zhang, Jianxin Zhao, Qixiao Zhai, Wei Chen, Fengwei Tian   +10 more
wiley   +1 more source

Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen

Bio-Protocol, 2014
The presence of intracellular glycogen can be detected by the following iodine staining technique. Cells with glycogen stain dark brown, whereas in its absence they remain with a pale yellowish color. It is hypothesized that iodine atoms fit into helical
Ana Demonte, Matías Diez, Sergio Guerrero, Miguel Ballicora, Alberto Iglesias   +4 more
doaj   +1 more source

SEC Mediates m6A Deposition and Transcription Pause Release to Drive Cell Identity Transition

Advanced Science, EarlyView.
The SEC promotes cell identity transitions by facilitating transcription pause release at key identity‐associated genes, including Klf4 and Myc during iPSC reprogramming and Nes and Tubb3 during ESC‐to‐neuroectodermal differentiation. During early iPSC reprogramming, this function additionally involves cooperation with METTL3‐associated m6A regulation.
Zhijing Zhang, Jingyi Xu, Yonghong Wu, Yitong Qu, Xinglin Hu, Yuchen Sun, Yanshuang Wu, Zhonghua Liu, Jiaqiang Wang, Lei Lei   +9 more
wiley   +1 more source

The nature of the primer for glycogen synthesis in muscle [PDF]

FEBS Letters, 1990
We and others have reported that glycogenin, the covalently bound protein found in muscle glycogen, also exists in muscle in a glycogen‐free form (M r, 38 000–39 000) that is autocatalytic, undergoes self‐glucosylation and acts as a primer for glycogen synthesis. We now report that this entity is not present in a fresh muscle extract. Instead it exists
Lomako, Joseph, Lomako, Wieslawa M., Whelan, William J.   +2 more
openaire   +2 more sources

Hepatocyte CBX2 Safeguards Hepatic Lipid Homeostasis by Elevating Ca2+‐CaMKK2‐AMPK Signaling to Counter MASLD

Advanced Science, EarlyView.
In hepatocytes, CBX2 promotes H3K4me3 enrichment at the Orai1 promoter to enhance Orai1 transcriptional activation, thereby increasing intracellular Ca2+ levels. The elevated Ca2+ activates the CaMKK2‐AMPK signaling cascade, resulting in ACC phosphorylation and downstream metabolic regulation. This cascade suppresses de novo lipogenesis (Fasn, Acc, Scd‐
Xiang Wei, Li Lin, Xiuyun Wang, Hui Du, Menglong Zhang, Kechao Tang, Han Gao, Xinrui Xie, Jingya Gao, Xu Wang, Zhenglin Wang, Siwei Huang, Lei Zhang, Fang Gu, Gong‐Hong Wei, Yinliang Qi, Wei Chen, Yongsheng Chang, Huabing Zhang   +18 more
wiley   +1 more source

Lithium‐Doped Hydroxyapatite Nanowires Rejuvenate Senescent Macrophages Via the Lysosome‐Dependent Ferroptosis Axis to Promote Aged Bone Regeneration

Advanced Science, EarlyView.
Li‐nHAP+GelMA promotes mandibular bone regeneration in aged mice by remodeling the senescent immune microenvironment. Local delivery of Li‐nHAP restores lysosomal homeostasis in senescent macrophages, reduces intracellular labile Fe2+ accumulation, ROS production, and lipid peroxidation, thereby suppressing macrophage ferroptosis and senescence ...
Baixi Chen, Ziduan Ding, Ruomei Li, Cheng Zhu, Zhiyu Zhu, Zitong Yang, Luyao Qu, Jin Hao, Lunguo Xia, Bing Fang, Lu Liu   +10 more
wiley   +1 more source

Cross‐Organ Multi‐Omics Profiling of Systemic Alterations in 5xFAD Mice and Changes Associated with Huanglian Jiedu Decoction Treatment

Advanced Science, EarlyView.
Cross‐organ multi‐omics reveals coordinated inflammatory and metabolic disturbances in 5xFAD mice. Huanglian Jiedu Decoction improves cognition and alleviates brain and liver abnormalities. Complementary hepatocyte–microglia conditioned‐medium experiments suggest PPAR‐γ involvement in treatment‐associated changes in microglial inflammation, providing ...
Wenya Gao, Jing Gao, Xiaohui Su, Xiangying Kong, Xiaolu Wei, Tunan Ding, Tiantian Guo, Shuangrong Gao, Yanyan Zhou, Junyi Zhou, Haiyu Zhao   +10 more
wiley   +1 more source

Sequestration of host metabolism by an intracellular pathogen

eLife, 2016
For intracellular pathogens, residence in a vacuole provides a shelter against cytosolic host defense to the cost of limited access to nutrients. The human pathogen Chlamydia trachomatis grows in a glycogen-rich vacuole.
Lena Gehre, Olivier Gorgette, Stéphanie Perrinet, Marie-Christine Prevost, Mathieu Ducatez, Amanda M Giebel, David E Nelson, Steven G Ball, Agathe Subtil   +8 more
doaj   +1 more source

Adaptive Cellular Responsiveness to Electrical Stimulation at the Skin‐Electrode Interface

Advanced Science, EarlyView.
This study presents an adaptive bioelectronic response in which sustained electrical stimulation (ES) upregulates voltage‐gated calcium channels (VGCCs) and enhances cellular sensitivity to subsequent ES at the skin‐device interface. VGCC‐associated adaptive sensitization promotes regenerative wound‐healing responses in vivo, including accelerated ...
Yeon Jae Chang, Soon Jin Choi, Khandoker Asiqur Rahaman, Tae Kyoung Lee, Gwangbin Lee, Jaeyeon Jun, Hyeon‐Jeong Lee, Hophil Min, Hojeong Jeon, Jung Keun Hyun, Hyung‐Seop Han, Jimin Park   +11 more
wiley   +1 more source