Results 21 to 30 of about 16,721 (247)

High-Throughput Glycomic Methods

Chemical Reviews, 2022
Glycomics aims to identify the structure and function of the glycome, the complete set of oligosaccharides (glycans), produced in a given cell or organism, as well as to identify genes and other factors that govern glycosylation. This challenging endeavor requires highly robust, sensitive, and potentially automatable analytical technologies for the ...
Irena Trbojević-Akmačić, Guinevere S. M. Lageveen-Kammeijer, Bram Heijs, Tea Petrović, Helena Deriš, Manfred Wuhrer, Gordan Lauc   +6 more
openaire   +4 more sources

Streptococcus pyogenes vaccine candidates do not induce autoimmune responses in a rheumatic heart disease model

npj Vaccines, 2023
We have developed a candidate vaccine to protect against multiple strains of Streptococcus pyogenes infections. The candidate vaccine contains two synthetic peptides derived from S. pyogenes proteins: the M-protein epitope, p*17 and the IL-8 degrading S.
Simone Reynolds, Rukshan Ahamed Mohamed Rafeek, Adam Hamlin, Ailin Lepletier, Manisha Pandey, Natkunam Ketheesan, Michael F. Good   +6 more
doaj   +1 more source

Streptolysin O Deficiency in Streptococcus pyogenes M1T1 covR/S Mutant Strain Attenuates Virulence in In Vitro and In Vivo Infection Models

mBio, 2023
Mutation within the Streptococcus pyogenes (Streptococcus group A; Strep A) covR/S regulatory system has been associated with a hypervirulent phenotype resulting from the upregulation of several virulence factors, including the pore-forming toxin ...
Emma L. Langshaw, Simone Reynolds, Victoria Ozberk, Jessica Dooley, Ainslie Calcutt, Mehfuz Zaman, Mark J. Walker, Michael R. Batzloff, Mark R. Davies, Michael F. Good, Manisha Pandey   +10 more
doaj   +1 more source

High-throughput mapping of RNA solvent accessibility at the single-nucleotide resolution by RtcB ligation between a fixed 5′-OH-end linker and unique 3′-P-end fragments from hydroxyl radical cleavage

RNA Biology, 2022
Given the challenges for the experimental determination of RNA tertiary structures, probing solvent accessibility has become increasingly important to gain functional insights.
Md Solayman, Thomas Litfin, Yaoqi Zhou, Jian Zhan   +3 more
doaj   +1 more source

Mass Spectrometry in the Elucidation of the Glycoproteome of Bacterial Pathogens [PDF]

, 2010
Presently some three hundred post-translational modifications are known to occur in bacteria in vivo. Many of these modifications play critical roles in the regulation of proteins and control key biological processes.
Graham, Robert L. J., Hess, Sonja
core   +1 more source

Towards a standardized bioinformatics infrastructure for N- and O-glycomics

Nature Communications, 2019
Glycomics is gaining momentum in basic, translational and clinical research. Here, the authors review current reporting standards and analysis tools for mass-spectrometry-based glycomics, and propose an e-infrastructure for standardized reporting and ...
Miguel A. Rojas-Macias, Julien Mariethoz, Peter Andersson, Chunsheng Jin, Vignesh Venkatakrishnan, Nobuyuki P. Aoki, Daisuke Shinmachi, Christopher Ashwood, Katarina Madunic, Tao Zhang, Rebecca L. Miller, Oliver Horlacher, Weston B. Struwe, Yu Watanabe, Shujiro Okuda, Fredrik Levander, Daniel Kolarich, Pauline M. Rudd, Manfred Wuhrer, Carsten Kettner, Nicolle H. Packer, Kiyoko F. Aoki-Kinoshita, Frédérique Lisacek, Niclas G. Karlsson   +23 more
doaj   +1 more source

Mosquito antiviral defense mechanisms: a delicate balance between innate immunity and persistent viral infection

Parasites & Vectors, 2019
Mosquito-borne diseases are associated with major global health burdens. Aedes spp. and Culex spp. are primarily responsible for the transmission of the most medically important mosquito-borne viruses, including dengue virus, West Nile virus and Zika ...
Wai-Suet Lee, Julie A. Webster, Eugene T. Madzokere, Eloise B. Stephenson, Lara J. Herrero   +4 more
doaj   +1 more source

Huisgen-based conjugation of water-soluble porphyrins to deprotected sugars: Towards mild strategies for the labelling of glycans [PDF]

, 2014
Fully deprotected alkynyl-functionalised mono- and oligosaccharides undergo CuAAC-based conjugation with water-soluble porphyrin azides in aqueous environments.
Boyle, Ross W., Bryden, Francesca, Daly, Robin, Giuntini, Francesca, Scanlan, Eoin M.   +4 more
core   +1 more source

The glycointeractome of serogroup B Neisseria meningitidis strain MC58

Scientific Reports, 2017
Neisseria meningitidis express numerous virulence factors that enable it to interact with diverse microenvironments within the host, during both asymptomatic nasopharyngeal colonization and invasive disease.
Tsitsi D. Mubaiwa, Lauren E. Hartley-Tassell, Evgeny A. Semchenko, Freda. E.-C. Jen, Yogitha N. Srikhanta, Christopher J. Day, Michael P. Jennings, Kate L. Seib   +7 more
doaj   +1 more source

N-glycolylneuraminic acid as a carbohydrate cancer biomarker

Translational Oncology, 2023
One of the forms of aberrant glycosylation in human tumors is the expression of N-glycolylneuraminic acid (Neu5Gc). The only known enzyme to biosynthesize Neu5Gc in mammals, cytidine-5′-monophosphate-N-acetylneuraminic acid (CMAH), appears to be ...
Jing Wang, Lucy K. Shewell, Christopher J. Day, Michael P. Jennings   +3 more
doaj   +1 more source