The Natural Product Corramycin Acts as a DNA Gyrase Poison and Overcomes Fluoroquinolone Resistance in Mycobacterium tuberculosis. [PDF]
Adv Sci (Weinh)Corramycin, a myxobacterial natural product antibiotic, exhibits potent bactericidal activity against multidrug‐resistant Mycobacterium tuberculosis. The compound hijacks bacterial transporters such as BacA and OppABCD to enter the cell and inhibits DNA synthesis through a previously unrecognized mode of DNA gyrase poisoning, locking the enzyme in an ...
Fries F +25 more
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Thermal Shifts Dominate Single-Gene Responses to Transcription-Targeting Antibiotics. [PDF]
Adv Sci (Weinh)Thermal shifts reshape bacterial responses to transcription‐targeting antibiotics. In E. coli, temperature can dominate antibiotic‐induced genome‐wide expression, particularly outside 30°C–39°C, through changes in adenosine triphosphate (ATP), nucleoid organization, and drug‐target engagement.
Jagadeesan R +5 more
europepmc +2 more sources
, 1999 DNA gyrase is a molecular machine comprising a series of protein gates. The opening and closing of these gates enables the passage of one segment of double-stranded DNA (the T segment) through a transient break in another (the G segment). We have blocked
Nicola Louise. Williams (7638380)
core +6 more sources
Frontiers in Microbiology, 2018 Persisters are the fraction of antibiotic-exposed bacteria transiently refractory to killing and are recognized as a cause of antibiotic treatment failure.
Shaunak Khandekar +8 more
doaj +1 more source
Clerocidin selectively modifies the gyrase-DNA gate to induce irreversible and reversible DNA damage [PDF]
, 2008 Clerocidin (CL), a microbial diterpenoid, reacts with DNA via its epoxide group and stimulates DNA cleavage by type II DNA topoisomerases. The molecular basis of CL action is poorly understood.
Fisher, LM +7 more
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A novel decatenation assay for DNA topoisomerases using a singly-linked catenated substrate
BioTechniques, 2020 Decatenation is a crucial in vivo reaction of DNA topoisomerases in DNA replication and is frequently used in in vitro drug screening. Usually this reaction is monitored using kinetoplast DNA as a substrate, although this assay has several limitations ...
Nidda F Waraich +5 more
doaj +1 more source
Antibacterial small molecules targeting the conserved TOPRIM domain of DNA gyrase. [PDF]
PLoS ONE, 2017 To combat the threat of antibiotic-resistant Gram-negative bacteria, novel agents that circumvent established resistance mechanisms are urgently needed.
Scott S Walker +20 more
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Structure of the N-terminal gyrase B fragment in complex with ADP⋅Pi reveals rigid-body motion induced by ATP hydrolysis [PDF]
, 2014 Type II DNA topoisomerases are essential enzymes that catalyze topological rearrangement of double-stranded DNA using the free energy generated by ATP hydrolysis.
Stanger, Frédéric V. +9 more
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RNA INTERFERNCE (RNAI) FOR SOME GENES FROM BABESIA BOVIS [PDF]
Research Journal of Applied Biotechnology, 2016 Babesia parasites are responsible for enormous economic losses to the livestock industry worldwide. There is no vaccine available to protect from the infection.
Ikuo Igarashi +2 more
doaj +1 more source
Adenylylation of Gyrase and Topo IV by FicT Toxins Disrupts Bacterial DNA Topology [PDF]
, 2015 Toxin-antitoxin (TA) modules are ubiquitous molecular switches controlling bacterial growth via the release of toxins that inhibit cell proliferation.
Gerdes, K. +27 more
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