Novel mouse model of colitis characterized by hapten-protein visualization
BioTechniques, 2010 Trinitrobenzene sulfonic acid (TNBS) and oxazolone are used to induce colitis for the investigation of inflammatory reactions in the colon. Although these chemicals are presumed to bind proteins in the colonic mucosa and then induce colitis as haptens ...
Kazuhiro Ishiguro +4 more
doaj +1 more source
Single-Domain Antibodies As Versatile Affinity Reagents for Analytical and Diagnostic Applications
Frontiers in Immunology, 2017 With just three CDRs in their variable domains, the antigen-binding site of camelid heavy-chain-only antibodies (HcAbs) has a more limited structural diversity than that of conventional antibodies.
Gualberto Gonzalez-Sapienza +2 more
doaj +1 more source
Assessment of Pre- and Pro-haptens Using Nonanimal Test Methods for Skin Sensitization [PDF]
, 2016 Because of ethical and regulatory reasons, several nonanimal test methods to assess the skin sensitization potential of chemicals have been developed and validated. In contrast to in vivo methods, they lack or provide limited metabolic capacity.
Daniel Urbisch (2630335) +15 more
core +1 more source
Hydrophobic Moiety of Capsaicinoids Haptens Enhancing Antibody Performance in Immunoassay: Evidence from Computational Chemistry and Molecular Recognition [PDF]
, 2021 We previously found that the immune response to haptens is positively correlated with molecular hydrophobicity. The antibodies used in immunoassays for capsaicinoids (CPCs) in waste oil suffer from low affinity and loose recognition to structural ...
Hui Jiang +21 more
core +1 more source
Dimerization kinetics of the IgE-class antibodies by divalent haptens. II. The interactions between intact IgE and haptens [PDF]
, 1992 Interactions between a monoclonal, DNP-specific IgE molecules (hybridoma A2) and divalent DNP-haptens in solution cause aggregation of the former predominantly into closed rings of two IgE and two divalent haptens (Schweitzer-Stenner, R., A.
Pecht, I. +2 more
core +1 more source
A New Type of Modified Brassinosteroids for Enzyme-linked Immunosorbent Assay
Natural Product Communications, 2008 New modified brassinosteroids (BS) with a full set of specific functional groups and with an additional one at C-26 have been synthesized and used as haptens for enzyme-linked immunosorbent assay. The haptens were conjugated to bovine serum albumin (BSA)
Vladimir Khripach +9 more
doaj +1 more source
[Translated article] Haptens, Proteins, and Atopic Dermatitis
Actas Dermo-Sifiliográficas, 2023 Atopic dermatitis is a chronic inflammatory disease that is multifactorial in nature. Allergic contact dermatitis and protein contact dermatitis are allergic conditions that may occur in the context of atopic dermatitis and be the cause of exacerbations.
V. Amat-Samaranch +1 more
doaj +1 more source
Journal of Lipid Research, 1972 Rabbit antisera to rat lymphosarcoma contain antibodies that are cytolytic for rat erythrocytes in the presence of complement. The reaction can be inhibited completely by pure cytolipin R showing that (a) immune hemolysis can be mediated through lipid ...
Keizo Inoue +2 more
doaj +1 more source
Synthesis of aminophosphonate haptens for an aminoacylation reaction between methyl glucoside and a β-alanyl ester [PDF]
, 2000 Two 2-aminophosphonate haptens derived from methyl α-d-glucopyranoside were synthesized to mimic the transition-state of a transesterification reaction between methyl α-d-glucopyranoside and 4-nitrophenylester of tert-BOC-β-alanine.
Yli-Kauhaluoma, Jari +3 more
core +1 more source
Extrinsic Cotton Effects in Hapten—Carrier and Hapten—Antibody Interactions [PDF]
Proceedings of the National Academy of Sciences, 1971 Two homogeneous univalent hapten-protein conjugates, prepared by the covalent attachment of a single 2,4-dinitrophenyl (DNP-) or 2,4,6-trinitrophenyl (TNP-) side chain to the cysteine-SH in the active site of the enzyme papain, have been found to exhibit large Cotton effects in the wavelength region of the absorption bands of the DNP or TNP groups ...
V, Reid +3 more
openaire +2 more sources