Results 31 to 40 of about 6,457,465 (175)
High-resolution cryo-EM structures of the E. coli hemolysin ClyA oligomers
bioRxiv, 2019 Pore-forming proteins (PFPs) represent a functionally important protein family, that are found in organisms from viruses to humans. As a major branch of PFPs, bacteria pore-forming toxins (PFTs) permeabilize membranes and usually cause the death of ...
W. Peng +4 more
semanticscholar +1 more source
Insights into protein sequencing with an α-Hemolysin nanopore by atomistic simulations [PDF]
Scientific Reports, 2019 AbstractSingle molecule protein sequencing would represent a disruptive burst in proteomic research with important biomedical impacts. Due to their success in DNA sequencing, nanopore based devices have been recently proposed as possible tools for the sequencing of peptide chains.
Di Muccio, Giovanni +4 more
openaire +3 more sources
SINGLE-MOLECULE DETECTION AND MANIPULATION WITH BIOLOGICAL NANOPORES
Studia Universitatis Babes-Bolyai Chemia, 2021 Single-molecule electrophysiology techniques using protein-based or solid-state nanopores as nanoreactors were proven incredibly useful as platforms for sensing and biophysical characterisation of biological molecules (e.g., peptides, proteins), DNA ...
Irina SCHIOPU +5 more
doaj +1 more source
Subunit Dimers of α-Hemolysin Expand the Engineering Toolbox for Protein Nanopores [PDF]
Journal of Biological Chemistry, 2011 Staphylococcal α-hemolysin (αHL) forms a heptameric pore that features a 14-stranded transmembrane β-barrel. We attempted to force the αHL pore to adopt novel stoichiometries by oligomerizing subunit dimers generated by in vitro transcription and translation of a tandem gene.
Hammerstein, A, Jayasinghe, L, Bayley, H
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Chalcone Attenuates Staphylococcus aureus Virulence by Targeting Sortase A and Alpha-Hemolysin
Frontiers in Microbiology, 2017 Staphylococcus aureus (S.aureus) resistance, considered a dilemma for the clinical treatment of this bacterial infection, is becoming increasingly intractable.
Bing Zhang +6 more
semanticscholar +1 more source
THE PROTEIN ASSOCIATED WITH HEMOLYSIN IN RABBIT SERUM AND PLASMA [PDF]
Journal of General Physiology, 1927 1. The water-insoluble globulin with which hemolysin is associated, may be separated from immune serum or plasma by dilution and simple dialysis at optimal pH. 2. This optimum in plasma is influenced by the presence of the fibrinogen. 3.
openaire +2 more sources
BMC Cancer, 2005 Background Serine-threonine kinases of the Raf family (A-Raf, B-Raf, C-Raf) are central players in cellular signal transduction, and thus often causally involved in the development of cancer when mutated or over-expressed.
Troppmair Jakob +6 more
doaj +1 more source
Crystal Structure of Hcp from Acinetobacter baumannii: A Component of the Type VI Secretion System. [PDF]
PLoS ONE, 2015 The type VI secretion system (T6SS) is a bacterial macromolecular machine widely distributed in Gram-negative bacteria, which transports effector proteins into eukaryotic host cells or other bacteria.
Federico M Ruiz +5 more
doaj +1 more source
Single-Molecule Study of Proteins by Biological Nanopore Sensors
Sensors, 2014 Nanopore technology has been developed for detecting properties of proteins through monitoring of ionic current modulations as protein passes via a nanosize pore.
Dongmei Wu +3 more
doaj +1 more source
Synthesized Geopolymers Adsorb Bacterial Proteins, Toxins, and Cells
Frontiers in Bioengineering and Biotechnology, 2020 Pore-forming and hemolytic toxins are bacterial cytotoxic proteins required for virulence in many pathogens, including staphylococci and streptococci, and are notably associated with clinical manifestations of disease.
John Popovich +10 more
doaj +1 more source