Results 111 to 120 of about 81,640 (295)

Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated virus (rAAV) is a widely used delivery vehicle in gene therapy. A scalable production technology is essential for its wide clinical applications. We have taken a synthetic biology approach to generate HEK293‐based cell lines which harbor integrated genetic elements encoding essential AAV and adenoviral helper ...
Han‐Jung Kuo, Prahalad Srinivasan, Yu‐Chieh Lin, Min Lu, Carissa Rungkittikhun, Qi Zhang, Wei‐Shou Hu   +6 more
wiley   +1 more source

Heparan Sulfate Proteoglycans Biosynthesis and Post Synthesis Mechanisms Combine Few Enzymes and Few Core Proteins to Generate Extensive Structural and Functional Diversity

Molecules, 2020
Glycosylation is a common and widespread post-translational modification that affects a large majority of proteins. Of these, a small minority, about 20, are specifically modified by the addition of heparan sulfate, a linear polysaccharide from the ...
Thibault Annaval, Rebekka Wild, Yoann Crétinon, Rabia Sadir, Romain R. Vivès, Hugues Lortat-Jacob   +5 more
doaj   +1 more source

Developing FGF2 Mutants with Selectively Reduced Heparan Sulfate Affinity to Explore Their Impact on FGFR1 Signaling

ChemBioChem, Accepted Article.
Fibroblast growth factor 2 (FGF2) regulates signal transduction by forming complexes with its receptors, FGF receptors (FGFRs), and heparan sulfate (HS), playing a crucial role in biological systems. Although HS has been suggested to modulate FGF/FGFR signaling as a co‐receptor, multiple hypotheses exist regarding how HS affects FGF/FGFR signaling, and
Yuga Okada, Akihiro Eguchi, Daisuke Kuroda, Kouhei Tsumoto, Ryosuke Ueki, Shinsuke Sando   +5 more
wiley   +1 more source

Heparan sulfate mimetics can efficiently mobilize long-term hematopoietic stem cells

Haematologica, 2012
Background Although mobilization of hematopoietic stem cells and hematopoietic progenitor cells can be achieved with a combination of granulocyte colony-stimulating factor and plerixafor (AMD3100), improving approaches for hematopoietic progenitor cell ...
Fabio Di Giacomo, Daniel Lewandowski, Eric Cabannes, Vanessa Nancy-Portebois, Maurice Petitou, Serge Fichelson, Paul-Henri Romeo   +6 more
doaj   +1 more source

Functional Cellular Anti-Tumor Mechanisms are Augmented by Genetic Proteoglycan Targeting. [PDF]

, 2020
While recent research points to the importance of glycans in cancer immunity, knowledge on functional mechanisms is lacking. In lung carcinoma among other tumors, anti-tumor immunity is suppressed; and while some recent therapies boost T-cell mediated ...
El Ghazal, Roland, Fuster, Mark M, Gupta, Purva, Johns, Scott C, Kim, So Young, Zuniga, Elina I   +5 more
core  

Can Conformers Be Identified via Cyclic Ion Mobility Mass Spectrometry? Hexosamine Epimers Reveal Ultrastable Ring Structures

Chemistry–Methods, EarlyView.
It was demonstrated how carbohydrate ring structures can be separated and identified using cyclic ion mobility mass spectrometry, making solid‐, solution‐, and gas‐phase structures comparable. In addition, it was determined which collisional cross‐section prediction or calculation method is the best suited for carbohydrates.
Viktória Goldschmidt Gőz, Imre Jákli, István Varga, Zsolt Dürvanger, Veronika Harmat, István Pintér, Gitta Schlosser, András Perczel   +7 more
wiley   +1 more source

Research Note: Effect of selection for body weight on the adipogenic conversion of turkey myogenic satellite cells by Syndecan-4 and its covalently attached N-glycosylation chains

Poultry Science, 2020
: Adult myoblasts, satellite cells, will proliferate, and differentiate into myotubes in vitro. However, changes in environmental and nutritional conditions will result in the satellite cells differentiating into adipocyte-like cells synthesizing lipids.
Sandra G. Velleman, Cynthia S. Coy
doaj  

Heparan Sulfate Identification and Characterisation: Method I. Heparan Sulfate Identification by NMR Analysis

Bio-Protocol, 2017
Heparin and heparan sulfate (HS) may be purified from complex biological matrices and are often isolated in sub-milligram quantities but not unequivocally identified by spectroscopic means.
Susan Carnachan, Simon F.R. Hinkley
doaj   +1 more source

RNA-Seq identifies SPGs as a ventral skeletal patterning cue in sea urchins [PDF]

, 2016
The sea urchin larval skeleton offers a simple model for formation of developmental patterns. The calcium carbonate skeleton is secreted by primary mesenchyme cells (PMCs) in response to largely unknown patterning cues expressed by the ectoderm.
Bardot, Evan, Blute, Todd A., Bradham, Cynthia A., Chaves, James, Chung, Oliver, Core, Amanda B., Coulombe-Huntington, Jasmin, Ferrell, Patrick, Fishman, Julie, Hameeduddin, Hajerah, Hewitt, Finnegan B., Hogan, John D., Ibn-Salem, Jonas, Keenan, Jessica L, Lee, David, Li, Christy, Luo, Lingqi, Oleinik, Ekaterina, Patel, Vijeta, Piacentino, Michael L., Poustka, Albert J., Ramachandran, Janani, Reyna, Arlene, Rose, Sviatlana, Speranza, Emily E., Yu, Jia, Zuch, Daniel T.   +26 more
core   +1 more source

The role of the PTHrP/Ihh feedback loop in the unusual growth plate location in mammalian metatarsals and pisiforms

Developmental Dynamics, EarlyView.
Abstract Background Longitudinal skeletal growth takes place in the cartilaginous growth plates. While growth plates are found at either end of conventional long bones, they occur at a variety of locations in the mammalian skeleton. For example, the metacarpals and metatarsals (MT) in the hands and feet form only a single growth plate at one end, and ...
Philip L. Reno, Sherrie Wallace, Sarah N. Doelp, Maria Biancaniello, Kelsey M. Kjosness   +4 more
wiley   +1 more source