Results 81 to 90 of about 75,723 (273)

Multiscale Architecture and Mechanics of the Cell Nucleus: Implications for Disease, Bioengineering and Nanomedicine

Advanced Science, EarlyView.
Nuclear mechanical properties are inherently scale‐dependent, arising from a hierarchical architecture that spans DNA, chromatin, the nuclear envelope, and condensates. Experimental techniques and theoretical models are integrated into a cohesive multiscale framework linking nanoscale structural features to organelle‐level mechanical behavior.
Xinran Liu, Xin Chen, Qingyang Zhao, Weikang Tan, Lingrui Zhu, Kin Liao, Vincent Chan, Nicolae Goga, Winfred O. Larkotey, Yang Li, Yinghui Shi, Wenya Shu, Lin Wang, Weiqing Wang, Tian Jian Lu, Yulong Han   +15 more
wiley   +1 more source

Correlative light and soft X-ray tomography of in situ mesoscale heterochromatin structure in intact cells

Scientific Reports
Heterochromatin organization is critical to many genome-related programs including transcriptional silencing and DNA repair. While super-resolution imaging, electron microscopy, and multiomics methods have provided indirect insights into the ...
Rajshikhar Gupta, Yagyik Goswami, Luezhen Yuan, Bibhas Roy, Eva Pereiro, GV Shivashankar   +5 more
doaj   +1 more source

Proteomic interrogation of human chromatin. [PDF]

, 2011
Chromatin proteins provide a scaffold for DNA packaging and a basis for epigenetic regulation and genomic maintenance. Despite understanding its functional roles, mapping the chromatin proteome (i.e. the "Chromatome") is still a continuing process. Here,
LeRoy, Gary, Christodoulos A Floudas, Floudas, Christodoulos A., Barry M Zee, Garcia, Benjamin A., Richard C Baliban, Baliban, Richard C., Sandra B Hake, Torrente, Mariana P., Zee, Barry M., Hake, Sandra B., Christodoulos A. Floudas, Barry M. Zee, Nicolas L. Young, Mariana P. Torrente, Gary LeRoy, Richard C. Baliban, Sandra B. Hake, Benjamin A. Garcia, Nicolas L Young, Benjamin A Garcia, Young, Nicolas L., Mariana P Torrente   +22 more
core   +1 more source

Phase transitions in heterochromatin organization

, 2023
International audienceHeterochromatin formation has been proposed to involve phase transitions on the level of the three-dimensional folding of heterochromatin regions and the liquid-liquid demixing of heterochromatin proteins.
Fabian Erdel, Erdel, Fabian
core   +1 more source

Mechanical Activation of Piezo1 Drives Osteoarthritis Through Kdm5c‐Mediated Epigenetic Silencing

Advanced Science, EarlyView.
Excessive mechanical stress activates Piezo1, triggering Ca2+‐dependent cytoskeletal forces that deform the nucleus and reduce H3K4me3. Kdm5c demethylates H3K4me3 at Col2a1 and Runx3 promoters. Kdm5c knockout rescues degradation. Repurposed telmisartan directly inhibits Kdm5c, blocking this axis and showing disease‐modifying efficacy in mouse OA models
Tianyou Kan, Xuran Li, Han Wang, Lin Sun, Yao Wang, Jiangdong Wu, Junqi Cui, Yiqi Yang, Kai Yuan, Linyang Chu, Liao Wang, Hanjun Li, Mengning Yan, Zhifeng Yu   +13 more
wiley   +1 more source

Kruppel-associated Box (KRAB)-associated co-repressor (KAP-1) Ser-473 phosphorylation regulates heterochromatin protein 1β (HP1-β) mobilization and DNA repair in heterochromatin

, 2012
The DNA damage response encompasses a complex series of signaling pathways that function to regulate and facilitate the repair of damaged DNA. Recent studies have shown that the repair of transcriptionally inactive chromatin, named heterochromatin, is ...
Savage, Kienan I, Mahen, Robert, Mahen, R., Graham, M. E., Venkitaraman, A. R., Bolderson, Emma, Pisupati, V., Khanna, K. K., Venkitaraman, Ashok R, Robinson, Phillip J, Khanna, Kum Kum, Graham, Mark E, Richard, Derek J, Pisupati, Venkat, Richard, Derek J., Robinson, P. J., Savage, K. I.   +16 more
core   +1 more source

NAD+ Metabolism Licenses Zygotic Genome Activation via PARP7‐Mediated ADP‐Ribosylation of UHRF1 in Mouse Early Embryos

Advanced Science, EarlyView.
This study uncovers a metabolic‐epigenetic axis licensing zygotic genome activation (ZGA) in mouse embryos. A developmental decline in NAD+ levels activates PARP7, which mono‐ADP‐ribosylates and stabilizes UHRF1. This modification promotes the establishment of permissive histone acetylation marks, thereby facilitating timely ZGA.
Guangyi Cao, Ziqi Zhang, Anqi Chen, Yanbo Liu, Yuling Lin, Lina Yu, Ruixin Shi, Aolei Guo, Yan Mao, Ganggui Lou, Chaojun Li, Luhong Wen, Guijun Yan, Haixiang Sun   +13 more
wiley   +1 more source

A strategy for mapping the heterochromatin of chromosome 2 of Drosophila melanogaster

, 2003
The heterochromatin of chromosome 2 of Drosophila melanogaster has been among the best characterized models for functional studies of heterochromatin owing to its abundance of genetic markers. To determine whether it might also provide a favorable system
J. C. Yasuhara, MARCHETTI, Marcella, FANTI, Laura, PIMPINELLI, Sergio, B. T. Wakimoto   +4 more
core   +1 more source

DHODH Drives Sunitinib Resistance Via a Non‐Enzymatic Mechanism by Inhibiting TRIM28 Ubiquitination and Consequent VEGFA Activation in RCC

Advanced Science, EarlyView.
This non‑enzymatic function of DHODH drives sunitinib resistance by competing with TRIM37 to block TRIM28 ubiquitination, thereby stabilizing TRIM28 and activating VEGFA transcription. Disrupting the DHODH–TRIM28 interaction with lisaftoclax restores drug sensitivity.
Shijie Qian, Shihang Pu, Zhipeng Xu, Yiheng Ding, Ling Jin, Baochao Li, Qi Li, Xiaohai Xi, Zengjun Wang, Jiajun Xing, Aiming Xu   +10 more
wiley   +1 more source

TOR targets an RNA processing network to regulate facultative heterochromatin, developmental gene expression and cell proliferation

, 2023
Cell proliferation and differentiation require signaling pathways that enforce appropriate and timely gene expression. We find that Tor2, the catalytic subunit of the TORC1 complex in fission yeast, targets a conserved nuclear RNA elimination network ...
Zofall, Martin, Pan, Lixia, Dhakshnamoorthy, Jothy, Lee, Nathan, Grewal, Shiv, Sun, Ling-ling, Wei, Yi, Wheeler, David   +7 more
core   +1 more source