Results 11 to 20 of about 229,438 (321)
New High-Throughput Screening Assays for Biocatalysis
CHIMIA, 2001 High-throughput screening for catalysis is a critical technology in all experiments aimed at modifying or creating enzymes by directed evolution, as well as for biodiversity mining for new catalysts.
Jean-Louis Reymond
doaj +4 more sources
Spectrophotometric Enzyme Assays for High-Throughput Screening
Food Technology and Biotechnology, 2004 This paper reviews high-throughput screening enzyme assays developed in our laboratory over the last ten years. These enzyme assays were initially developed for the purpose of discovering catalytic antibodies by screening cell culture supernatants, but ...
Jean-Louis Reymond
doaj +2 more sources
siMacro: A Fast and Easy Data Processing Tool for Cell-Based Genomewide siRNA Screens [PDF]
Genomics & Informatics, 2013 Growing numbers of studies employ cell line-based systematic short interfering RNA (siRNA) screens to study gene functions and to identify drug targets.
Nitin Kumar Singh +4 more
doaj +1 more source
FLICK: An optimized plate reader-based assay to infer cell death kinetics
STAR Protocols, 2021 Summary: Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity.
Ryan Richards +2 more
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A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
STAR Protocols, 2022 Summary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with ...
Benjamin Louis Sievers +2 more
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RyR1-targeted drug discovery pipeline integrating FRET-based high-throughput screening and human myofiber dynamic Ca2+ assays. [PDF]
, 2020 Elevated cytoplasmic [Ca2+] is characteristic in severe skeletal and cardiac myopathies, diabetes, and neurodegeneration, and partly results from increased Ca2+ leak from sarcoplasmic reticulum stores via dysregulated ryanodine receptor (RyR) channels ...
Bers, Donald M +6 more
core +2 more sources
Multiplex Profiling of Cellular Invasion in 3D Cell Culture Models. [PDF]
, 2012 To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces.
Burgstaller, Gerald +4 more
core +8 more sources
Screening and High-Throughput Platelet Assays [PDF]
, 2018 High-throughput assays are important biological research tools but are rarely utilized for platelet research. However, screening compounds for efficacy against a physiologically relevant cellular response in primary cells such as platelets can be an advantageous approach to compound screening and drug development. In this section we describe a panel of
Bye, Alexander P +2 more
openaire +3 more sources
Assessment of protein inclusions in cultured cells using automated image analysis
STAR Protocols, 2022 Summary: Proteinaceous inclusions are associated with neurodegenerative diseases and cell models are often used to determine genetic and chemical modifiers of their formation.
Luke McAlary +6 more
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