Mining Chemical Activity Status from High-Throughput Screening Assays. [PDF]
PLoS One, 2015 High-throughput screening (HTS) experiments provide a valuable resource that reports biological activity of numerous chemical compounds relative to their molecular targets.
Soufan O +6 more
europepmc +3 more sources
Development of High Throughput Screening Assays Using Fluorescence Polarization: Nuclear Receptor-Ligand–Binding and Kinase /Phosphatase Assays [PDF]
Journal of biomolecular screening, 2000 Fluorescence polarization (FP) has been used to develop high throughput screening (HTS) assays for nuclear receptor-ligand displacement and kinase inhibition.
G.J. Parker +3 more
openalex +2 more sources
New High-Throughput Screening Assays for Biocatalysis
CHIMIA, 2001 High-throughput screening for catalysis is a critical technology in all experiments aimed at modifying or creating enzymes by directed evolution, as well as for biodiversity mining for new catalysts.
Jean-Louis Reymond
doaj +4 more sources
Identification of Compounds That Inhibit Estrogen-Related Receptor Alpha Signaling Using High-Throughput Screening Assays. [PDF]
Molecules, 2019 The nuclear receptor, estrogen-related receptor alpha (ERRα; NR3B1), plays a pivotal role in energy homeostasis. Its expression fluctuates with the demands of energy production in various tissues.
Lynch C +8 more
europepmc +2 more sources
Integrated Model of Chemical Perturbations of a Biological Pathway Using 18 In Vitro High-Throughput Screening Assays for the Estrogen Receptor. [PDF]
Toxicol Sci, 2015 We demonstrate a computational network model that integrates 18 in vitro, high-throughput screening assays measuring estrogen receptor (ER) binding, dimerization, chromatin binding, transcriptional activation, and ER-dependent cell proliferation.
Judson RS +18 more
europepmc +2 more sources
A Data Analysis Pipeline Accounting for Artifacts in Tox21 Quantitative High-Throughput Screening Assays. [PDF]
J Biomol Screen, 2015 A main goal of the U.S. Tox21 program is to profile a 10K-compound library for activity against a panel of stress-related and nuclear receptor signaling pathway assays using a quantitative high-throughput screening (qHTS) approach.
Hsieh JH +4 more
europepmc +2 more sources
Big data in chemical toxicity research: the use of high-throughput screening assays to identify potential toxicants. [PDF]
Chem Res Toxicol, 2014 High-throughput screening (HTS) assays that measure the in vitro toxicity of environmental compounds have been widely applied as an alternative to in vivo animal tests of chemical toxicity.
Zhu H +5 more
europepmc +2 more sources
Apoptosis assessment in high-content and high-throughput screening assays
BioTechniques, 2021 Here the authors describe the development of AUTOptosis, an economical and rapid apoptosis monitoring method suitable for high-content and high-throughput screening assays.
Céline Rens +5 more
doaj +1 more source
Using in vitro high throughput screening assays to identify potential endocrine-disrupting chemicals. [PDF]
Environ Health Perspect, 2013 Background: Over the past 20 years, an increased focus on detecting environmental chemicals that pose a risk of adverse effects due to endocrine disruption has driven the creation of the U.S.
Rotroff DM +11 more
europepmc +2 more sources
siMacro: A Fast and Easy Data Processing Tool for Cell-Based Genomewide siRNA Screens [PDF]
Genomics & Informatics, 2013 Growing numbers of studies employ cell line-based systematic short interfering RNA (siRNA) screens to study gene functions and to identify drug targets.
Nitin Kumar Singh +4 more
doaj +1 more source