Alginate-based 3D cancer cell culture for therapeutic response modeling
STAR Protocols, 2021 Summary: Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients.
Farideh Davoudi +9 more
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Clinical application of high throughput molecular screening techniques for pharmacogenomics. [PDF]
, 2011 Genetic analysis is one of the fastest-growing areas of clinical diagnostics. Fortunately, as our knowledge of clinically relevant genetic variants rapidly expands, so does our ability to detect these variants in patient samples.
Schrijver, Iris, Wiita, Arun P
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Frontiers in Microbiology, 2023 Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public ...
Samuele Stazzoni +7 more
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Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring. [PDF]
, 2019 The new and rapid advancement in the complexity of biologics drug discovery has been driven by a deeper understanding of biological systems combined with innovative new therapeutic modalities, paving the way to breakthrough therapies for previously ...
Chen, Ching +17 more
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Iterative focused screening with biological fingerprints identifies selective Asc-1 inhibitors distinct from traditional high throughput screening [PDF]
, 2017 N-methyl-d-aspartate receptors (NMDARs) mediate glutamatergic signaling that is critical to cognitive processes in the central nervous system, and NMDAR hypofunction is thought to contribute to cognitive impairment observed in both schizophrenia and ...
Amoss, Adam +18 more
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Recent developments in the rapid analysis of plants and tracking their bioactive constituents [PDF]
, 2009 Natural products chemistry has witnessed many new developments in the last 5 years like extractions with subcritical water and ionic liquids, LC/HRMS and LC/SPE/cryo-NMR, UHPLC, TLC/MS, MS-based preparative HPLC, comprehensive chromatography (GC × GC, LC
Beek, T.A., van +7 more
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Advances in the application of high-throughput microfluidic platforms in precise diagnosis and treatment of tumors [PDF]
精准医学杂志Although the treatment of malignant tumors has entered an era of comprehensive therapy integrating chemotherapy, targeted therapy, immunotherapy, and other modalities, there is still a significant gap from individualized precise treatment.
DOU Liliang, CHU Jiahui, LIU Lian
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Methods to identify the unexplored diversity of microbial exopolysaccharides
Frontiers in Microbiology, 2015 Microbial exopolysaccharides (EPS) are a structurally very diverse class of molecules. A number of them have found their application in rather diverging fields that extend from medicine, food and cosmetics on the one side to construction, drilling and ...
Broder eRühmann +2 more
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STAR Protocols, 2021 Summary: Here, we describe an optimized protocol to identify specific nuclear receptor ligands. First, to rule out any compound interference with luciferase activity per se, we describe an in vitro assay assessing potential inhibition or activation of ...
Alexandre Berthier +2 more
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Canvass: a crowd-sourced, natural-product screening library for exploring biological space [PDF]
, 2018 NCATS thanks Dingyin Tao for assistance with compound characterization. This research was supported by the Intramural Research Program of the National Center for Advancing Translational Sciences, National Institutes of Health (NIH). R.B.A.
Andrade, Rodrigo B. +94 more
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