Results 31 to 40 of about 227,287 (224)

Methods to identify the unexplored diversity of microbial exopolysaccharides

Frontiers in Microbiology, 2015
Microbial exopolysaccharides (EPS) are a structurally very diverse class of molecules. A number of them have found their application in rather diverging fields that extend from medicine, food and cosmetics on the one side to construction, drilling and ...
Broder eRühmann, Jochen eSchmid, Volker eSieber   +2 more
doaj   +1 more source

Combined whole-cell high-throughput functional screening for identification of new nicotinamidases/pyrazinamidases in metagenomic/polygenomic libraries

Frontiers in Microbiology, 2016
Nicotinamidases catalyze the hydrolysis of the amide bond in nicotinamide to produce ammonia and nicotinic acid. These enzymes are an essential component of the NAD+ salvage pathway and are implicated in the viability of several pathogenic organisms. Its
Rubén Zapata-Pérez, Antonio Ginés García-Saura, Mohamed Jebbar, Peter Golyshin, Álvaro Sánchez-Ferrer   +4 more
doaj   +1 more source

A system for performing high throughput assays of synaptic function. [PDF]

PLoS ONE, 2011
Unbiased, high-throughput screening has proven invaluable for dissecting complex biological processes. Application of this general approach to synaptic function would have a major impact on neuroscience research and drug discovery.
Chris M Hempel, Michael Sivula, Jonathan M Levenson, David M Rose, Bing Li, Ana C Sirianni, Eva Xia, Timothy A Ryan, David J Gerber, Jeffrey R Cottrell   +9 more
doaj   +1 more source

FLICK: An optimized plate reader-based assay to infer cell death kinetics

STAR Protocols, 2021
Summary: Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity.
Ryan Richards, Megan E. Honeywell, Michael J. Lee   +2 more
doaj  

Arbitrarily Accessible 3D Microfluidic Device for Combinatorial High-Throughput Drug Screening

Sensors, 2016
Microfluidics-based drug-screening systems have enabled efficient and high-throughput drug screening, but their routine uses in ordinary labs are limited due to the complexity involved in device fabrication and system setup.
Zhuofa Chen, Weizhi Li, Gihoon Choi, Xiaonan Yang, Jun Miao, Liwang Cui, Weihua Guan   +6 more
doaj   +1 more source

Embracing assay heterogeneity with neural processes for markedly improved bioactivity predictions [PDF]

arXiv, 2023
Predicting the bioactivity of a ligand is one of the hardest and most important challenges in computer-aided drug discovery. Despite years of data collection and curation efforts by research organizations worldwide, bioactivity data remains sparse and heterogeneous, thus hampering efforts to build predictive models that are accurate, transferable and ...
arxiv  

A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay

STAR Protocols, 2022
Summary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with ...
Benjamin Louis Sievers, Terri Gelbart, Gene S. Tan   +2 more
doaj  

Development of a High Throughput Platform for Screening Glycoside Hydrolases based on Oxime-NIMS

Frontiers in Bioengineering and Biotechnology, 2015
Cost effective hydrolysis of biomass into sugars for biofuel production requires high-performance low-cost glycoside hydrolase (GH) cocktails that are active under demanding process conditions.
Kai eDeng, Kai eDeng, Joel M Guenther, Joel M Guenther, Jian eGao, Benjamin Paul Bowen, Huu eTran, Huu eTran, Vimalier eReyes-Ortiz, Vimalier eReyes-Ortiz, Xiaoliang eCheng, Xiaoliang eCheng, Noppadon eSathitsuksanoh, Noppadon eSathitsuksanoh, Richard eHeins, Richard eHeins, Taichi E Takasuka, Lai eBergeman, Henrik eGeertz-Hansen, Henrik eGeertz-Hansen, Sam eDeutsch, Sam eDeutsch, Dominique eLoqué, Dominique eLoqué, Kenneth eSale, Kenneth eSale, Blake eSimmons, Blake eSimmons, Paul D Adam, Paul D Adam, Paul D Adam, Anup K Singh, Anup K Singh, Brian Grant Fox, Brian Grant Fox, Trent Russell Northen, Trent Russell Northen   +36 more
doaj   +1 more source

Optimization of Spectrophotometric and Fluorometric Assays Using Alternative Substrates for the High-Throughput Screening of Lipase Activity

Journal of Chemistry, 2021
The effects of reaction conditions on the spectrophotometric and fluorometric assays using alternative substrates (p-nitrophenyl palmitate and 4-methylumbelliferyl oleate) were investigated to optimize them for the high-throughput screening of lipase ...
Jun-Young Park, Jisu Ha, Yoonseok Choi, Pahn-Shick Chang, Kyung-Min Park   +4 more
doaj   +1 more source

Scalable lipid droplet microarray fabrication, validation, and screening [PDF]

arXiv, 2022
High throughput screening of small molecules and natural products is costly, requiring significant amounts of time, reagents, and operating space. Although microarrays have proven effective in the miniaturization of screening for certain biochemical assays, such as nucleic acid hybridization or antibody binding, they are not widely used for drug ...
arxiv