Results 51 to 60 of about 229,438 (321)

Quantify permeability using on-a-chip models in high-throughput applications

STAR Protocols, 2023
Summary: Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%).
Camilla Soragni, Tessa Vergroesen, Nynke Hettema, Gwenaëlle Rabussier, Henriëtte L. Lanz, Sebastian J. Trietsch, Leon J. de Windt, Chee P. Ng   +7 more
doaj   +1 more source

Inhibitors of SARS-CoV entry--identification using an internally-controlled dual envelope pseudovirion assay. [PDF]

, 2011
Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged as the causal agent of an endemic atypical pneumonia, infecting thousands of people worldwide.
Agudelo, Juliet, Amberg, Sean M, Chen, Yen Ting, Craik, Charles S, Goetz, David H, Hansell, Elizabeth, Lu, Kai, McKerrow, James, Roush, William R, Simmons, Graham, Zhou, Yanchen   +10 more
core   +1 more source

Image-based dynamic phenotyping reveals genetic determinants of filamentation-mediated beta-lactam tolerance [PDF]

, 2020
Antibiotic tolerance characterized by slow killing of bacteria in response to a drug can lead to treatment failure and promote the emergence of resistance.
Camacho, Rafael, Fauvart, Maarten, Franke, Sabine, Hofkens, Johan, Marchal, Kathleen, Michielsen, Jan, Weytjens, Bram, Wilmaerts, Dorien, Zahir, Taiyeb   +8 more
core   +2 more sources

Quantification of drug-induced fractional killing using high-throughput microscopy

STAR Protocols, 2021
Summary: Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging.
Zintis Inde, Jason Rodencal, Scott J. Dixon   +2 more
doaj   +1 more source

Identification of Small Molecules that Enhance Synaptogenesis Using Synapse Microarrays [PDF]

, 2013
Synaptic function is affected in many brain diseases and disorders. Technologies for large-scale synapse assays can facilitate identification of drug leads.
Ghosh, Balaram, Haggarty, Stephen John, Mazitschek, Ralph, Scott, Mark A, Shi, Peng, Wan, Dongpeng, Wissner-Gross, Zachary, Yanik, Mehmet Fatih   +7 more
core   +1 more source

A high-throughput and highly automated genotoxicity screening assay

ALTEX, 2021
The increasing number of compounds under development and chemicals in commerce that require safety assessments pose a serious challenge for regulatory agencies worldwide. In vitro screening using toxicogenomic biomarkers has been proposed as a first-tier screen in chemical assessment and has been endorsed internationally.
Renxiang, Chen, Yun-Tien, Lin, Albert J, Fornace, Heng-Hong, Li   +3 more
openaire   +2 more sources

High-throughput drug screening of fine-needle aspiration-derived cancer organoids

STAR Protocols, 2020
Summary: Generation of fine-needle aspiration (FNA)-derived cancer organoids has allowed us to develop a number of downstream applications. In this protocol, we start with organoids cultured in a semi-solid format.
Kensey Bergdorf, Courtney Phifer, Vijaya Bharti, David Westover, Joshua Bauer, Anna Vilgelm, Ethan Lee, Vivian Weiss   +7 more
doaj   +1 more source

High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphology

STAR Protocols, 2022
Summary: Well-characterized small molecules enable the study of cell processes and facilitate target validation. Here, we describe a high-content multiplex screen to investigate cell viability over 48 h, which can be combined with investigating ...
Amelie Tjaden, Robert T. Giessmann, Stefan Knapp, Martin Schröder, Susanne Müller   +4 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Protocol to identify receptors of secreted proteins through CRISPR-Cas9 whole-genome screening technology

STAR Protocols
Summary: The interaction between cell surface receptors and their ligands is crucial for intercellular communication. However, current techniques for identifying direct receptor-ligand interactions remain limited.
Xiaoli Hu, Yiguo Wang
doaj   +1 more source