Results 61 to 70 of about 231,965 (320)
Development of a High Throughput Platform for Screening Glycoside Hydrolases based on Oxime-NIMS
Frontiers in Bioengineering and Biotechnology, 2015 Cost effective hydrolysis of biomass into sugars for biofuel production requires high-performance low-cost glycoside hydrolase (GH) cocktails that are active under demanding process conditions.
Kai eDeng +36 more
doaj +1 more source
High-throughput drug screening of fine-needle aspiration-derived cancer organoids
STAR Protocols, 2020 Summary: Generation of fine-needle aspiration (FNA)-derived cancer organoids has allowed us to develop a number of downstream applications. In this protocol, we start with organoids cultured in a semi-solid format.
Kensey Bergdorf +7 more
doaj +1 more source
Frontiers in Cellular and Infection Microbiology, 2022 Schistosomiasis is a parasitic neglected disease with praziquantel (PZQ) utilized as the main drug for treatment, despite its low effectiveness against early stages of the worm. To aid in the search for new drugs to tackle schistosomiasis, computer-aided
Bernardo Pereira Moreira +7 more
doaj +1 more source
High-throughput screening in larval zebrafish identifies novel potent sedative-hypnotics [PDF]
, 2018 BACKGROUND: Many general anesthetics were discovered empirically, but primary screens to find new sedative-hypnotics in drug libraries have not used animals, limiting the types of drugs discovered.
Brown, Lauren E. +12 more
core +1 more source
An integrated molecular and conventional breeding scheme for enhancing genetic gain in maize in Africa [PDF]
, 2020 Open Access Journal; Published online: 06 Nov 2019Maize production in West and Central Africa (WCA) is constrained by a wide range of interacting stresses that keep productivity below potential yields.
Gedil, M., Menkir, A.
core +1 more source
Peptide‐based ligand antagonists block a Vibrio cholerae adhesin
FEBS Letters, EarlyView.The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang +9 more
wiley +1 more source
Protocol to identify small-molecule inhibitors against cancer drug resistance
STAR ProtocolsSummary: Drug resistance has emerged as a critical challenge in clinical cancer treatment. Here, we present a high-throughput screening protocol to identify therapeutic small-molecule inhibitors against drug-resistant cancer cells.
Juan Wei +6 more
doaj +1 more source
STAR Protocols, 2021 Summary: Histone deacetylases (HDACs) are ubiquitous enzymes that cleave post-translational ε-N-acyllysine modifications. The continued identification of diverse acyl modifications at lysine residues in proteins has resulted in discovery of new insight ...
Carlos Moreno-Yruela, Christian A. Olsen
doaj +1 more source
High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines. [PDF]
, 2016 Hundreds of genetically characterized cell lines are available for the discovery of genotype-specific cancer vulnerabilities. However, screening large numbers of compounds against large numbers of cell lines is currently impractical, and such experiments
A Basu +45 more
core +2 more sources
Robust Analysis of High Throughput Screening (HTS) Assay Data [PDF]
Technometrics, 2013 Quantitative high throughput screening (qHTS) assays use cells or tissues to screen thousands of compounds in a short period of time. Data generated from qHTS assays are then evaluated using nonlinear regression models, such as the Hill model, and decisions regarding toxicity are made using the estimates of the parameters of the model.
Changwon, Lim +2 more
openaire +2 more sources