Results 61 to 70 of about 229,438 (321)

Development of a High Throughput Platform for Screening Glycoside Hydrolases based on Oxime-NIMS

Frontiers in Bioengineering and Biotechnology, 2015
Cost effective hydrolysis of biomass into sugars for biofuel production requires high-performance low-cost glycoside hydrolase (GH) cocktails that are active under demanding process conditions.
Kai eDeng, Kai eDeng, Joel M Guenther, Joel M Guenther, Jian eGao, Benjamin Paul Bowen, Huu eTran, Huu eTran, Vimalier eReyes-Ortiz, Vimalier eReyes-Ortiz, Xiaoliang eCheng, Xiaoliang eCheng, Noppadon eSathitsuksanoh, Noppadon eSathitsuksanoh, Richard eHeins, Richard eHeins, Taichi E Takasuka, Lai eBergeman, Henrik eGeertz-Hansen, Henrik eGeertz-Hansen, Sam eDeutsch, Sam eDeutsch, Dominique eLoqué, Dominique eLoqué, Kenneth eSale, Kenneth eSale, Blake eSimmons, Blake eSimmons, Paul D Adam, Paul D Adam, Paul D Adam, Anup K Singh, Anup K Singh, Brian Grant Fox, Brian Grant Fox, Trent Russell Northen, Trent Russell Northen   +36 more
doaj   +1 more source

Gametocyte-specific and all-blood-stage transmission-blocking chemotypes discovered from high throughput screening on Plasmodium falciparum gametocytes

Communications Biology, 2022
High-throughput screening of 120,000 compounds followed by counter-screening and validation assays reveals candidate antimalarial drugs that kill Plasmodium falciparum sexual and asexual blood stages blocking parasite transmission through mosquito.
Giacomo Paonessa, Giulia Siciliano, Rita Graziani, Cristiana Lalli, Ottavia Cecchetti, Cristina Alli, Roberto La Valle, Alessia Petrocchi, Alessio Sferrazza, Monica Bisbocci, Mario Falchi, Carlo Toniatti, Alberto Bresciani, Pietro Alano   +13 more
doaj   +1 more source

Recent developments in the rapid analysis of plants and tracking their bioactive constituents [PDF]

, 2009
Natural products chemistry has witnessed many new developments in the last 5 years like extractions with subcritical water and ionic liquids, LC/HRMS and LC/SPE/cryo-NMR, UHPLC, TLC/MS, MS-based preparative HPLC, comprehensive chromatography (GC × GC, LC
Beek, T.A., van, Claassen, F.W., Dapkevicius, A., Exarchou, V., Jeurissen, S.M.F., Klift, E.J.C., van der, Koleva, I., Tetala, K.K.R.   +7 more
core   +2 more sources

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Protocol to identify small-molecule inhibitors against cancer drug resistance

STAR Protocols
Summary: Drug resistance has emerged as a critical challenge in clinical cancer treatment. Here, we present a high-throughput screening protocol to identify therapeutic small-molecule inhibitors against drug-resistant cancer cells.
Juan Wei, She-Yu Zhang, Xue-Yuan Zhou, Yong Wei, Hao-Ran Jia, Qin Wu, Weihong Tan   +6 more
doaj   +1 more source

High-throughput screening assay used in pharmacognosy: Selection, optimization and validation of methods of enzymatic inhibition by UV-visible spectrophotometry [PDF]

Journal of Pharmacy & Pharmacognosy Research, 2014
In research laboratories of both organic synthesis and extraction of natural products, every day a lot of products that can potentially introduce some biological activity are obtained.
Graciela Granados-Guzmán, Noemí Waksman de Torres, Rocío Castro-Ríos, Ricardo Salazar-Aranda   +3 more
doaj  

High-throughput screening of histone deacetylases and determination of kinetic parameters using fluorogenic assays

STAR Protocols, 2021
Summary: Histone deacetylases (HDACs) are ubiquitous enzymes that cleave post-translational ε-N-acyllysine modifications. The continued identification of diverse acyl modifications at lysine residues in proteins has resulted in discovery of new insight ...
Carlos Moreno-Yruela, Christian A. Olsen
doaj   +1 more source

Two rapid assays for screening of patulin biodegradation [PDF]

, 2013
Artículo sobre distintos ensayos para comprobar la biodegradación de la patulinaThe mycotoxin patulin is produced by the blue mould pathogen Penicillium expansum in rotting apples during postharvest storage.
A Ciegler, A Ciegler, A Halász, A Ricelli, AB Gonçalves, EE Stinson, ER McKinley, G Hageskal, G Hageskal, G Lima, IE Yates, J Acar, J Harwig, J He, J Reiss, K Gromadzka, M Vansteelandt, MM Moake, MO Moss, MS Anderson, N Pérez-Guerra, NB Sammon, P Karlovsky, PM Scott, QK Wu, R Castoria, R Castoria, R Castoria, R Castoria, R Castoria, R Fliege, R Steiman, S MacDonald, SAI Wright, T Robinson, VG Lilly, VJ Pereira, VM Siqueira, ZL Sumbu   +38 more
core   +3 more sources

Robust Analysis of High Throughput Screening (HTS) Assay Data [PDF]

Technometrics, 2013
Quantitative high throughput screening (qHTS) assays use cells or tissues to screen thousands of compounds in a short period of time. Data generated from qHTS assays are then evaluated using nonlinear regression models, such as the Hill model, and decisions regarding toxicity are made using the estimates of the parameters of the model.
Changwon, Lim, Pranab K, Sen, Shyamal D, Peddada   +2 more
openaire   +2 more sources

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source