Results 61 to 70 of about 227,287 (224)
Journal of biomolecular screening, 2005 HIV-1 integrase (HIV-IN) is a well-validated antiviral drug target catalyzing a multistep reaction to incorporate the HIV-1 provirus into the genome of the host cell.
Yu Wang +12 more
semanticscholar +1 more source
Informed Pooled Testing with Quantitative Assays [PDF]
arXiv, 2020 Pooled testing is widely used for screening for viral or bacterial infections with low prevalence when individual testing is not cost-efficient. Pooled testing with qualitative assays that give binary results has been well-studied. However, characteristics of pooling with quantitative assays were mostly demonstrated using simulations or empirical ...
arxiv
Journal of biomolecular screening, 2008 Kinases are important drug discovery targets for a wide variety of therapeutic indications; consequently, the measurement of kinase activity remains a common high-throughput screening (HTS) application.
T. Schröter +15 more
semanticscholar +1 more source
STAR Protocols, 2021 Summary: Here, we describe an optimized protocol to identify specific nuclear receptor ligands. First, to rule out any compound interference with luciferase activity per se, we describe an in vitro assay assessing potential inhibition or activation of ...
Alexandre Berthier +2 more
doaj
International Journal of High Throughput Screening, 2010 Drug development for many diseases would be aided greatly by accurate in vitro model systems that replicate key elements of in vivo physiology. The recent development of co-culture systems of endothelial cells and vascular smooth muscle cells can be ...
G. Truskey
semanticscholar +1 more source
Is Group Testing Ready for Prime-time in Disease Identification? [PDF]
arXiv, 2020 Large scale disease screening is a complicated process in which high costs must be balanced against pressing public health needs. When the goal is screening for infectious disease, one approach is group testing in which samples are initially tested in pools and individual samples are retested only if the initial pooled test was positive.
arxiv
Recent Advances in Design of Fluorescence-Based Assays for High-Throughput Screening.
Analytical Chemistry, 2018 Significant improvements in disease management have been achieved in the past few decades. Using cancer as an example, survival rates have increased dramatically in economically developed countries, according to the World Cancer Report published by WHO ...
Xiaoni Fang +4 more
semanticscholar +1 more source
A High Throughput Apoptosis Assay using 3D Cultured Cells
Molecules, 2019 A high throughput apoptosis assay using 3D cultured cells was developed with a micropillar/microwell chip platform. Live cell apoptosis assays based on fluorescence detection have been useful in high content screening.
Sang-Yun Lee, Il Doh, Dong Woo Lee
doaj +1 more source
A microfluidic platform for high-throughput multiplexed protein quantitation [PDF]
, 2014 We present a high-throughput microfluidic platform capable of quantitating up to 384 biomarkers in 4 distinct samples by immunoassay. The microfluidic device contains 384 unit cells, which can be individually programmed with pairs of capture and detection antibody.
arxiv +1 more source
Inhibition of self-splicing group I intron RNA: high-throughput screening assays.
Nucleic Acids Research, 1996 High-throughput screening assays have been developed to rapidly identify small molecule inhibitors targeting catalytic group I introns. Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis carinii or from ...
H. Mei +5 more
semanticscholar +1 more source