Results 71 to 80 of about 231,965 (320)

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Protocol for screening inhibitors of the fungal sphingolipid-metabolizing enzyme ceramide synthase

STAR Protocols
Summary: Fungal sphingolipids have emerged as potent antifungal targets based on cumulative evidence demonstrating that fungal sphingolipid metabolism is essential to the virulence of pathogenic fungi. Here, we present a protocol to screen for inhibitors
Andy Zhong, Deveney Dasilva, Nivea Pereira de Sa, Jeehyun Karen You, Maurizio Del Poeta   +4 more
doaj   +1 more source

Determination of Creatinine in Human Urine with Flow Injection Tandem Mass Spectrometry [PDF]

, 2012
Background/Aims: Excretion of urinary compounds in spot urine is often estimated relative to creatinine. For the growing number of liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays of urine-excreted molecules, a fast and accurate method ...
Koletzko, Berthold, Niesser, Mareile, Peissner, Wolfgang   +2 more
core   +1 more source

Image-based dynamic phenotyping reveals genetic determinants of filamentation-mediated beta-lactam tolerance [PDF]

, 2020
Antibiotic tolerance characterized by slow killing of bacteria in response to a drug can lead to treatment failure and promote the emergence of resistance.
Camacho, Rafael, Fauvart, Maarten, Franke, Sabine, Hofkens, Johan, Marchal, Kathleen, Michielsen, Jan, Weytjens, Bram, Wilmaerts, Dorien, Zahir, Taiyeb   +8 more
core   +2 more sources

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Organoid drug screening in cancer: advances in high-throughput platforms and clinical applications [PDF]

Organoid
Patient-derived tumor organoids represent the most physiologically relevant 3-dimensional (3D) cancer models, preserving tumor heterogeneity and native tissue architecture.
Jiho Lee, Hyun Ju Kang, Jin-Ku Lee
doaj   +1 more source

Time after time – circadian clocks through the lens of oscillator theory

FEBS Letters, EarlyView.
Oscillator theory bridges physics and circadian biology. Damped oscillators require external drivers, while limit cycles emerge from delayed feedback and nonlinearities. Coupling enables tissue‐level coherence, and entrainment aligns internal clocks with environmental cues.
Marta del Olmo, Carolin Ector, Hanspeter Herzel   +2 more
wiley   +1 more source

Gametocyte-specific and all-blood-stage transmission-blocking chemotypes discovered from high throughput screening on Plasmodium falciparum gametocytes

Communications Biology, 2022
High-throughput screening of 120,000 compounds followed by counter-screening and validation assays reveals candidate antimalarial drugs that kill Plasmodium falciparum sexual and asexual blood stages blocking parasite transmission through mosquito.
Giacomo Paonessa, Giulia Siciliano, Rita Graziani, Cristiana Lalli, Ottavia Cecchetti, Cristina Alli, Roberto La Valle, Alessia Petrocchi, Alessio Sferrazza, Monica Bisbocci, Mario Falchi, Carlo Toniatti, Alberto Bresciani, Pietro Alano   +13 more
doaj   +1 more source

The SARS-coronavirus-host interactome [PDF]

, 2011
Coronaviruses (CoVs) are important human and animal pathogens that induce fatal respiratory, gastrointestinal and neurological disease. The outbreak of the severe acute respiratory syndrome (SARS) in 2002/2003 has demonstrated human vulnerability to ...
Brunn, Albrecht von, Carbajo-Lozoya, Javier, Dall'Armi, Ekatarina von, Ditt, Vanessa, Drosten, Christian, Friedel, Caroline C., Haas, Juergen G., Herrler, Georg, Herzog, Petra, Hilgenfeld, Rolf, Kallies, Stefan, Kuri, Thomas, Kögl, Manfred, Müller, Marcel A., Niemeyer, Daniela, Pfefferle, Susanne, Pumpor, Ksenia, Pöhlmann, Stefan, Schwarz, Frank, Schwegmann-Wessels, Christel, Schöpf, Julia, Steffen, Imke, Stellberger, Thorsten, Thiel, Heinz-Jürgen, Thiel, Volker, Weber, Friedemann, Zimmer, Ralf, Züst, Roland   +27 more
core   +1 more source

A Rapid Transglutaminase Assay for High-Throughput Screening Applications [PDF]

SLAS Discovery, 2006
Transglutaminases (TGs) are widely distributed enzymes that catalyze posttranslational modification of proteins by Ca(2+)-dependent cross-linking reactions. The family members of TGs participate in many significant processes of biological functions such as tissue regeneration, cell differentiation, apoptosis, and certain pathologies.
Yu-Wei, Wu, Yu-Hui, Tsai
openaire   +2 more sources