Results 71 to 80 of about 227,287 (224)
STAR Protocols, 2022 Summary: Well-characterized small molecules enable the study of cell processes and facilitate target validation. Here, we describe a high-content multiplex screen to investigate cell viability over 48 h, which can be combined with investigating ...
Amelie Tjaden +4 more
doaj
Quantification of drug-induced fractional killing using high-throughput microscopy
STAR Protocols, 2021 Summary: Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging.
Zintis Inde +2 more
doaj
Moving microfluidics ahead: Extending capabilities, accessibility, and applications [PDF]
arXiv, 2018 Paul Blainey is professor of Biological Engineering at MIT. In this contribution he describes three microfluidic technologies that he and his team has developed to extend the capability, accessibility, and applications of microfluidics: (1) Integrated microfluidic sample preparation for genomic assays, (2) hydrogel-based microfluidics for single-cell ...
arxiv
Quantify permeability using on-a-chip models in high-throughput applications
STAR Protocols, 2023 Summary: Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%).
Camilla Soragni +7 more
doaj
Journal of Inborn Errors of Metabolism and Screening, 2016 All newborn screening laboratories in the United States and many worldwide screen for cystic fibrosis. Most laboratories use a second-tier genotyping assay to identify a panel of mutations in the CF transmembrane regulator ( CFTR ) gene.
Miyono M. Hendrix MS +2 more
doaj +1 more source
Analytics for metabolic engineering
Frontiers in Bioengineering and Biotechnology, 2015 Realizing the promise of metabolic engineering has been slowed by challenges related to moving beyond proof-of-concept examples to robust and economically viable systems. Key to advancing metabolic engineering beyond trial-and-error research is access to
Christopher J Petzold +4 more
doaj +1 more source
High-throughput drug screening of fine-needle aspiration-derived cancer organoids
STAR Protocols, 2020 Summary: Generation of fine-needle aspiration (FNA)-derived cancer organoids has allowed us to develop a number of downstream applications. In this protocol, we start with organoids cultured in a semi-solid format.
Kensey Bergdorf +7 more
doaj
STAR Protocols, 2021 Summary: Histone deacetylases (HDACs) are ubiquitous enzymes that cleave post-translational ε-N-acyllysine modifications. The continued identification of diverse acyl modifications at lysine residues in proteins has resulted in discovery of new insight ...
Carlos Moreno-Yruela, Christian A. Olsen
doaj
A cGMP-Dependent Protein Kinase Assay for High Throughput Screening Based on Time-Resolved Fluorescence Resonance Energy Transfer [PDF]
, 2001 Benjamin Bader +5 more
openalex +2 more sources
Efficient pooling designs for library screening [PDF]
arXiv, 1994 We describe efficient methods for screening clone libraries, based on pooling schemes which we call ``random $k$-sets designs''. In these designs, the pools in which any clone occurs are equally likely to be any possible selection of $k$ from the $v$ pools. The values of $k$ and $v$ can be chosen to optimize desirable properties.
arxiv