Results 81 to 90 of about 227,287 (224)
Protocol to identify small-molecule inhibitors against cancer drug resistance
STAR ProtocolsSummary: Drug resistance has emerged as a critical challenge in clinical cancer treatment. Here, we present a high-throughput screening protocol to identify therapeutic small-molecule inhibitors against drug-resistant cancer cells.
Juan Wei +6 more
doaj
STAR Protocols, 2022 Summary: Discovery of small molecules promoting cardiomyocyte proliferation is important for heart regeneration and related heart disease. Here, we describe a protocol to isolate neonatal rat and mouse cardiomyocytes, infect cardiomyocytes with Tnnt2-mAG-
Lixia Zheng +4 more
doaj
Frontiers in Microbiology, 2018 This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux.
Sara M. El Kheir +13 more
doaj +1 more source
Quantification of heparin in complex matrices (including urine) using a mix-and-read fluorescence assay [PDF]
arXiv, 2016 Heparin is an important anticoagulant drug, about one billion doses are produced annually. It is a polydisperse sulfated polysaccharide, and the inherent heterogeneity makes the analysis of heparin difficult. The global crisis resulting from adulterated heparin in 2008 has drawn renewed attention to the challenges that are associated with the quality ...
arxiv
Development of a p38 Kinase Binding Assay for High Throughput Screening [PDF]
, 1999 Usha Warrior +11 more
openalex +1 more source
STAR ProtocolsSummary: The interaction between cell surface receptors and their ligands is crucial for intercellular communication. However, current techniques for identifying direct receptor-ligand interactions remain limited.
Xiaoli Hu, Yiguo Wang
doaj
Assay Miniaturization for Ultra-High Throughput Screening of Combinatorial and Discrete Compound Libraries: A 9600-Well (0.2 Microliter) Assay System [PDF]
, 1998 Kevin R. Oldenburg +6 more
openalex +1 more source
PLoS ONE, 2014 Three-dimensional cell culture has many advantages over monolayer cultures, and spheroids have been hailed as the best current representation of small avascular tumours in vitro.
D. Ivanov +6 more
semanticscholar +1 more source
In cellulo and in vivo assays for compound testing against Trypanosoma cruzi
STAR Protocols, 2023 Summary: Here, we describe a combined in cellulo and in vivo approach to identify compounds with higher potential for efficient inhibition of Trypanosoma cruzi.
Eloise Pavão Guerra-Slompo +5 more
doaj
Fluorescence Polarization Assays for High Throughput Screening of G Protein–Coupled Receptors [PDF]
, 2000 Peter Banks +2 more
openalex +1 more source