Results 91 to 100 of about 7,899 (213)
Penulenan dan penjujukan protein hirudin daripada lintah tempatan (hirudinaria manillensis) bagi penghasilan protein hirudin secara skala besar pada peringkat industri [PDF]
, 1993 Fokus penyelidikan ini adalah untuk menulenkan protein hirudin daripada lintah tempatan, Hirudinaria manillensis. Hirudin adalah antikoagulan semulajadi yang dirembeskan oleh kelenjar liur lintah. Ia mempunyai kepentingan dalam bidang perubatan kerana ia
Abdul Latif, Fazilah, Salim, Norizah
core
, 2001 Recombinant Saccharomyces cerevisiae strains were developed to overproduce an anticoagulant hirudin. The δ-sequences of the yeast retrotransposon Ty1 and URA3 were used as target sites for a hirudin expression cassette.
Sang-Ki Rhee +5 more
core +1 more source
Crystal Structure of a Biosynthetic Sulfo-hirudin Complexed to Thrombin
, 2016 The leech-derived anticoagulant hirudin is post-translationally sulfated on tyrosine 63, resulting in a >10-fold increase in its affinity for thrombin.
Peter G. Schultz (183240) +3 more
core +1 more source
Protective Effect and Molecular Mechanism of Hirudin on Kidney of Diabetic Rats
Xiehe Yixue ZazhiObjective To investigate the protective effect of hirudin on kidneys of diabetic rats and its molecular mechanism. Methods Eighteen Sprague-Dawley rats were randomly divided into control group (n=5) and model group (n=13).
HE Yiqian +4 more
doaj +1 more source
Hirudin in heparin-induced thrombocytopenia
, 2002 Heparin-induced thrombocytopenia (HIT), a serious side effect of heparin treatment, requires alternative anticoagulation in most affected patients. The recombinant hirudin (r-hirudin) lepirudin has been approved for this purpose after two prospective ...
Greinacher, Andreas, Lubenow, Norbert,
core +1 more source
, 2011 A recombinant targeting bifunctional hirudin was expressed in the yeast Pichia pastoris. In order to decrease the side effects of hirudin and increase its activity to prevent arterial thrombus, we fused a factor Xa (FXa) recognition sequence into N’ of ...
Deng, X +5 more
core +1 more source
The Properties of Scrambled Hirudins
Journal of Biological Chemistry, 1995 Scrambled hirudins serve as relevant folding intermediates during in vitro renaturation of the reduced/denatured hirudin. Eleven species of scrambled hirudins were isolated and structurally identified. Their properties, including their relative stability and the kinetics of their consolidation (disulfide reshuffling) to attain the native structure ...
openaire +2 more sources
, 2009 [[abstract]]Hirudin, isolated from the European leech Hirudo medicinalis, is a potent inhibitor of thrombin, forming an almost irreversible thrombin-hirudin complex.
Mao SJT;Yates MT;Owen TJ;Krstenansky JL
core
陆军军医大学学报Objective To construct vitamin E (tocopherol, VE)-based liposomes with both hydrogen peroxide-scavenging function and anticoagulant effect, and explore whether they can be used to treat acute ischemic stroke and then evaluate their in vitro anticoagulant
YANG Qinghua +2 more
doaj +1 more source
Interaction of α2-macroglobulin-bound thrombin with hirudin
, 1984 The human thrombin bound to α2-macroglobulin (α2 M) in a 1:1 stoichiometry is still able to interact with one of its specific inhibitors, hirudin. The dissociation constant of the complex hirudin-α2M-bound thrombin is 1 × 10−7 M, whatever the mode of ...
Steinbuch, Marion +3 more
core +1 more source