Identification and Characterization of Holin-like Protein ORF70 from Cyanophage MaMV-DC [PDF]
Marine DrugsIn this study, we characterized the holin-like protein ORF70 from the cyanophage MaMV-DC, offering valuable insights into its role in phage-mediated host cell lysis.
Lihui Meng +5 more
doaj +4 more sources
Prophages in Skin Pathogens: From Virulence to Therapy [PDF]
PathogensProphages are bacteriophage genomes that are part of bacterial chromosomes. They are not just dormant passengers; they actively shape pathogen biology.
Abirami Karthikeyan +7 more
doaj +3 more sources
Molecular Cloning and Functional Analysis of Holin and Endolysin From Escherichia Phage UE‐M6 as Potential Antibacterial Agents [PDF]
MicrobiologyOpenThe rise of antibiotic‐resistant bacteria has intensified the search for alternative antibacterial strategies. Bacteriophage (phage) therapy is gaining attention as a promising approach, utilizing phage‐derived proteins such as holins and endolysins to ...
Hira Niaz, Mikael Skurnik, Fazal Adnan
doaj +3 more sources
Structural and Genetic Diversity of Lysis Modules in Bacteriophages Infecting the Genus Streptococcus [PDF]
Genes (Basel)Bacteriophages infecting the genus Streptococcus play a crucial role in microbial ecology and have potential applications in biotechnology and medicine. Despite their importance, significant gaps remain in our understanding of their lysis modules.
M. Saint-Jean +3 more
semanticscholar +3 more sources
Thiol Protection in Membrane Protein Purifications: A Study With Phage Holins [PDF]
Analytical Biochemistry, 2009 The lambda holin, or S105, is a small cytoplasmic membrane protein that controls the timing of host lysis. Using thiol-specific reagents, we determined that the single cysteine residue within S105 was heterogeneously modified during membrane extraction ...
Ry Young, Douglas K Struck, Jill S Dewey
exaly +4 more sources
Genetic analysis of F1 cluster phages that infect Mycobacterium smegmatis identifies two distinct holin-like proteins that regulate the host lysis event. [PDF]
PLoS ONEPhages Girr and NormanBulbieJr (NBJ) infect Gram-positive Mycobacterium smegmatis mc2 155. Both phages contain conserved lysis cassettes that harbor two endolysin genes (lysin A and lysin B) and two genes encoding transmembrane domain (TMD) holin-like ...
Richard S Pollenz +3 more
doaj +3 more sources
Directed evolution of a staphylophage under biofilm and planktonic conditions
npj Biofilms and MicrobiomesThe rise of multidrug-resistant bacteria, particularly biofilm-forming pathogens such as Staphylococcus epidermidis, highlights the urgent need for alternative antimicrobial strategies.
Carlos Valdivia, Pilar Domingo-Calap
doaj +3 more sources
Holins Kill Without Warning [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2001 Holins comprise the most diverse functional group of proteins known. They are small bacteriophage-encoded proteins that accumulate during the period of late-protein synthesis after infection and cause lysis of the host cell at a precise genetically programmed time.
Ry Young, Michael D Manson
exaly +4 more sources
Active Bax and Bak Are Functional Holins [PDF]
Genes and Development, 2011 The mechanism of Bax/Bak-dependent mitochondrial outer membrane permeabilization (MOMP), a central apoptotic event primarily controlled by the Bcl-2 family proteins, remains not well understood. Here, we express active Bax/Bak in bacteria, the putative origin of mitochondria, and examine their functional similarities to the λ ...
Casey M Gries, Samir H Moussa, Xu Luo
exaly +4 more sources
Occurrence and potential mechanism of holin-mediated non-lytic protein translocation in bacteria [PDF]
Microbial Cell, 2022 Holins are generally believed to generate large membrane lesions that permit the passage of endolysins across the cytoplasmic membrane of prokaryotes, ultimately resulting in cell wall degradation and cell lysis. However, there are more and more examples
Thomas Brüser, Denise Mehner-Breitfeld
doaj +2 more sources