Multiple holins contribute to extracellular DNA release in Pseudomonas aeruginosa biofilms [PDF]
bioRxiv, 2020 Bacterial biofilms are comprised of aggregates of cells encased within a matrix of extracellular polymeric substances (EPS). One key EPS component is extracellular DNA (eDNA), which acts as a ‘glue’, facilitating cell-cell and cell-substratum ...
Amelia L Hynen +6 more
semanticscholar +2 more sources
A holin/peptidoglycan hydrolase‐dependent protein secretion system [PDF]
Molecular Microbiology, 2020 AbstractGram‐negative bacteria have evolved numerous pathways to secrete proteins across their complex cell envelopes. Here, we describe a protein secretion system that uses a holin membrane protein in tandem with a cell wall‐editing enzyme to mediate the secretion of substrate proteins from the periplasm to the cell exterior.
Tracy Palmer +4 more
openaire +4 more sources
Characterization and Therapeutic Potential of a Novel Bacteriophage Targeting Methicillin‐Resistant Staphylococcus aureus Biofilms on Medical Devices [PDF]
International Journal of Microbiology, Volume 2026, Issue 1, 2026.Methicillin‐resistant Staphylococcus aureus (MRSA) is a major cause of multidrug‐resistant and biofilm‐associated infections, particularly on indwelling medical devices, where conventional antibiotics often exhibit limited efficacy. In this study, we isolated and characterized a novel lytic bacteriophage, SA_SGEB_01, targeting MRSA from hospital ...
Raphael Kabir Niloy +11 more
wiley +2 more sources
Topological and phylogenetic analyses of bacterial holin families and superfamilies [PDF]
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2013 Holins are small "hole-forming" transmembrane proteins that mediate bacterial cell lysis during programmed cell death or following phage infection. We have identified fifty two families of established or putative holins and have included representative members of these proteins in the Transporter Classification Database (TCDB; www.tcdb.org).
Reddy, Bhaskara L, Saier, Milton H
openaire +5 more sources
CidA and LrgA: a “Hole” Lot More than Programmed Cell Death
mBio, 2022 What do programmed cell death (PCD) and carbohydrate metabolism by-product transport have in common? Intriguingly, both processes involve the cidABC and lrgAB operons in the major human pathogen Staphylococcus aureus.
Maisem Laabei, Seána Duggan
doaj +2 more sources
Signature Prediction Modeling in Cytolethal Distending Toxin-Producing Strains [PDF]
Genomics & Informatics, 2017 In this study, cytolethal distending toxin (CDT) producer isolates genome were compared with genome of pathogenic and commensal Escherichia coli strains. Conserved genomic signatures among different types of CDT producer E. coli strains were assessed. It
Maryam Javadi +2 more
doaj +2 more sources
Pepholin, a bacteriophage holin-derived antimicrobial peptide with membrane-disruptive activity and therapeutic efficacy in MDR Pseudomonas aeruginosa burn wounds [PDF]
RSC AdvancesThe emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa poses a serious threat to burn wound healing, necessitating the development of alternative therapeutics beyond conventional antibiotics.
Purushotham RV +8 more
doaj +2 more sources
From Cell Death to Metabolism: Holin-Antiholin Homologues with New Functions [PDF]
mBio, 2017 Programmed cell death in bacteria is generally triggered by membrane proteins with functions analogous to those of bacteriophage holins: they disrupt the membrane potential, whereas antiholins antagonize this process.
Marielle H. van den Esker +2 more
doaj +2 more sources
Bacteriophage-mediated biofilm control: a novel targeted strategy for the management of dental caries [PDF]
Frontiers in Cellular and Infection MicrobiologyDental caries, a globally prevalent chronic infectious disease, is driven by microbial dysbiosis of dental biofilm, involving multiple caries-associated colonizers.
Huijie Sun +9 more
doaj +2 more sources
A Cytoplasmic Antiholin Is Embedded In Frame with the Holin in a Lactobacillus fermentum Bacteriophage [PDF]
Applied and Environmental Microbiology, 2018 ABSTRACT In double-stranded DNA bacteriophages, infection cycles are ended by host cell lysis through the action of phage-encoded endolysins and holins. The precise timing of lysis is regulated by the holin inhibitors, named antiholins.
Tingting, Guo +3 more
openaire +3 more sources