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Molecular Cloning and Functional Analysis of Holin and Endolysin From Escherichia Phage UE-M6 as Potential Antibacterial Agents [PDF]

MicrobiologyOpen
The rise of antibiotic‐resistant bacteria has intensified the search for alternative antibacterial strategies. Bacteriophage (phage) therapy is gaining attention as a promising approach, utilizing phage‐derived proteins such as holins and endolysins to ...
Mikael Skurnik, Fazal Adnan
exaly   +3 more sources

Identification and Characterization of Holin-like Protein ORF70 from Cyanophage MaMV-DC [PDF]

Marine Drugs
In this study, we characterized the holin-like protein ORF70 from the cyanophage MaMV-DC, offering valuable insights into its role in phage-mediated host cell lysis.
Lihui Meng, Yi Wu, Jiahao Xu, Jiarui Zhang, Zhiyong Zhang, Chen Wang   +5 more
doaj   +3 more sources

Biological function identification of phage holin Hol-4086 and treatment of Staphylococcus aureus infection [PDF]

Frontiers in Microbiology
The treatment of infections caused by drug-resistant Staphylococcus aureus has become increasingly difficult. In this study, the complete genome of phage 4086-1 against S. aureus was sequenced and shown to be 17,960 bp in size, with a GC content of 29.14%
Guiwei Li, Ruichong Wang, Yanping Jiang
exaly   +4 more sources

Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process

Frontiers in Microbiology, 2021
Bacteriophage T7 gene 17.5 coding for the only known holin is one of the components of its lysis system, but the holin activity in T7 is more complex than a single gene, and evidence points to the existence of additional T7 genes with holin activity.
Hafizah Y. Chenia, Jibo Hou, Roshini Govinden   +2 more
exaly   +3 more sources

Biological functional identification of holins from Salmonella pullorum phages and their therapeutic efficacy against pullorum disease in chickens [PDF]

Poultry Science
Salmonella pullorum (S.pullorum) is a significant pathogen that seriously endangers the poultry industry. However, its prevention and control mainly rely on antibiotics, which leads to the wide spread of drug-resistant genes and poses a serious public ...
Huyang Zhao, Wei Wu, Hongzhe Zhao, Chenyu Zheng, Fei Teng, Mingze Chen, Jiaxuan Li, Yanping Jiang, Wen Cui, Yingying Ma, Xinyuan Qiao   +10 more
doaj   +2 more sources

Assessment of the phi6 lysis system using genetic complementation and heterologous expression in Escherichia coli and Pseudomonas syringae [PDF]

Frontiers in Microbiology
The RNA cystovirus phi6 represents a unique evolutionary outlier, encoding a holin and endolysin similar to tailed phages, yet lacking an identified gene for outer membrane disruption.
Devyn Del Curto, Micaela Zamora, Greyson Lasley, Rabia Khan, Katlyn Montalbano, J. Bryce Ricken, Jesse Cahill   +6 more
doaj   +2 more sources

Co-expression of holin gene improves heterologous cellulase secretion and surface display by lactic acid bacteria Lactococcus cremoris [PDF]

Journal of Biological Engineering
Background Genetically modified Lactococcus cremoris strains producing heterologous cellulases emerge as promising candidates for cost-effective bioprocessing of plant biowaste into high-value organic compounds.
Petra Štravs, Lara Repar, Henri-Pierre Fierobe, Stéphanie Perret, Aleš Berlec   +4 more
doaj   +2 more sources

The Lysis cassette of jumbophage PhiKZ [PDF]

Scientific Reports
Pseudomonas jumbophage phiKZ is prominent because of the proteinaceous nuclear structure that is formed during the infection cycle, protecting the replicating phage DNA from host nucleases.
Prasanth Manohar, Joshua Wan, Gabriella Ganser, Kiersten Sharp, Ry Young   +4 more
doaj   +2 more sources

The short cytoplasmic region of phage T4 holin is essential for the transition from impermeable membrane protein complexes to permeable pores [PDF]

Frontiers in Microbiology
Virulent as well as temperate phages usually require lysis of bacteria to release their progeny into the surrounding. Double-stranded DNA phages achieve lysis by phage-encoded endolysins that degrade the bacterial cell wall.
Jan Michel Frederik Schwarzkopf, Ruth Paola Viveros, Ali Nazmi Burdur, Denise Mehner-Breitfeld, Natalia Tschowri, Thomas Brüser   +5 more
doaj   +2 more sources

Identification and analysis of the crucial holin domain and sites and the bactericidal activity of a holin–endolysin lysis cassette from phage PZL-Ah152 against Aeromonas hydrophila [PDF]

Journal of Virology
With the increasing prevalence of antibiotic-resistant bacteria, phage therapy has garnered significant attention. Holin and lysin play essential roles in the phage-induced lysis of bacteria. This study investigated the functions of the holin protein Hol
Chao Feng, Yan Cheng, Shuang Liang, Ruiqi Liang, Jiahao Yu, Shun Wang, Hui Guo, Xiaofeng Shan, Dongxing Zhang, Aidong Qian, Wuwen Sun, Lei Zhang   +11 more
doaj   +2 more sources