Results 31 to 40 of about 6,534 (190)

Holins kill without warning [PDF]

Proceedings of the National Academy of Sciences, 2001
Holins comprise the most diverse functional group of proteins known. They are small bacteriophage-encoded proteins that accumulate during the period of late-protein synthesis after infection and cause lysis of the host cell at a precise genetically programmed time.
A, Gründling, M D, Manson, R, Young
openaire   +2 more sources

Active Bax and Bak are functional holins [PDF]

Genes & Development, 2011
The mechanism of Bax/Bak-dependent mitochondrial outer membrane permeabilization (MOMP), a central apoptotic event primarily controlled by the Bcl-2 family proteins, remains not well understood. Here, we express active Bax/Bak in bacteria, the putative origin of mitochondria, and examine their functional similarities to the λ ...
Xiaming, Pang, Samir H, Moussa, Natalie M, Targy, Jeffrey L, Bose, Nicholas M, George, Casey, Gries, Hernando, Lopez, Liqiang, Zhang, Kenneth W, Bayles, Ry, Young, Xu, Luo   +10 more
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Purification and Biochemical Characterization of the Lambda Holin [PDF]

Journal of Bacteriology, 1998
ABSTRACT Holins are small phage-encoded cytoplasmic membrane proteins, remarkable for their ability to make membranes permeable in a temporally regulated manner. The purification of S105, the λ holin, and one of the two products of gene S is described.
D L, Smith, D K, Struck, J M, Scholtz, R, Young   +3 more
openaire   +2 more sources

Peptidoglycan-Free Bacterial Ghosts Confer Enhanced Protection against Yersinia pestis Infection

Vaccines, 2021
To develop a modern plague vaccine, we used hypo-endotoxic Yersinia pestis bacterial ghosts (BGs) with combinations of genes encoding the bacteriophage ɸX174 lysis-mediating protein E and/or holin-endolysin systems from λ or L-413C phages.
Svetlana V. Dentovskaya, Anastasia S. Vagaiskaya, Mikhail E. Platonov, Alexandra S. Trunyakova, Sergei A. Kotov, Ekaterina A. Krasil’nikova, Galina M. Titareva, Elizaveta M. Mazurina, Tat’yana V. Gapel’chenkova, Rima Z. Shaikhutdinova, Sergei A. Ivanov, Tat’yana I. Kombarova, Vladimir N. Gerasimov, Vladimir N. Uversky, Andrey P. Anisimov   +14 more
doaj   +1 more source

Mycobacteriophage D29 holin C‐terminal region functionally assists in holin aggregation and bacterial cell death [PDF]

The FEBS Journal, 2015
Holins are phage‐encoded small transmembrane proteins that perforate the bacterial cytoplasmic membrane. In most cases, this process allows the phage‐encoded peptidoglycan hydrolases to act on the cell wall, resulting in host cell lysis and phage release.
Soumya, Kamilla, Vikas, Jain
openaire   +2 more sources

Endolysins of bacteriophage vB_Sal-S-S10 can naturally lyse Salmonella enteritidis

BMC Veterinary Research, 2022
Background The holin-endolysin lysis system plays an essential role in the phage life cycle. Endolysins are promising alternatives to antibiotics, and have been successfully used against Gram-positive bacteria.
Xinrui Wang, Lili Han, Jiaqing Rong, Huiying Ren, Wenhua Liu, Can Zhang   +5 more
doaj   +1 more source

Characterization and Therapeutic Potential of a Novel Bacteriophage Targeting Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms on Medical Devices. [PDF]

Int J Microbiol
Methicillin‐resistant Staphylococcus aureus (MRSA) is a major cause of multidrug‐resistant and biofilm‐associated infections, particularly on indwelling medical devices, where conventional antibiotics often exhibit limited efficacy. In this study, we isolated and characterized a novel lytic bacteriophage, SA_SGEB_01, targeting MRSA from hospital ...
Niloy RK, Mondal SI, Jewel NA, Zaeem I, Mahin MR, Karim D, Rolin MH, Khan T, Jubair M, Rahman M, Akter A.   +10 more
europepmc   +2 more sources

A holin/peptidoglycan hydrolase‐dependent protein secretion system [PDF]

Molecular Microbiology, 2020
AbstractGram‐negative bacteria have evolved numerous pathways to secrete proteins across their complex cell envelopes. Here, we describe a protein secretion system that uses a holin membrane protein in tandem with a cell wall‐editing enzyme to mediate the secretion of substrate proteins from the periplasm to the cell exterior.
Tracy Palmer, Alexander J. Finney, Chayan Kumar Saha, Gemma C. Atkinson, Frank Sargent   +4 more
openaire   +3 more sources

Optimum Threshold Minimizes Noise in Timing of Intracellular Events

iScience, 2020
Summary: How the noisy expression of regulatory proteins affects timing of intracellular events is an intriguing fundamental problem that influences diverse cellular processes.
Sherin Kannoly, Tianhui Gao, Supravat Dey, Ing-Nang Wang, Abhyudai Singh, John J. Dennehy   +5 more
doaj   +1 more source

Identification and Characterization of a New Type of Holin-Endolysin Lysis Cassette in Acidovorax oryzae Phage AP1

Viruses, 2022
Phages utilize lysis systems to allow the release of newly assembled viral particles that kill the bacterial host. This is also the case for phage AP1, which infects the rice pathogen Acidovorax oryzae.
Muchen Zhang, Yanli Wang, Jie Chen, Xianxian Hong, Xinyan Xu, Zhifeng Wu, Temoor Ahmed, Belinda Loh, Sebastian Leptihn, Sabry Hassan, Mohamed M. Hassan, Guochang Sun, Bin Li   +12 more
doaj   +1 more source