Results 51 to 60 of about 6,534 (190)
Sizing the Holin Lesion with an Endolysin-β-Galactosidase Fusion [PDF]
Journal of Bacteriology, 2003 ABSTRACT Double-stranded DNA phages require two proteins for efficient host lysis: the endolysin, a muralytic enzyme, and the holin, a small membrane protein. In an event that defines the end of the vegetative cycle, the λ holin S acts suddenly to permeabilize the membrane. This permeabilization enables the R endolysin to attack the cell wall,
Ing-Nang, Wang, John, Deaton, Ry, Young
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Solubilization and delivery by GroEL of megadalton complexes of the λ holin [PDF]
Protein Science, 2004 AbstractGroEL can solubilize membrane proteins by binding them in its hydrophobic cavity when detergent is removed by dialysis. The best‐studied example is bacteriorhodopsin, which can bind in the GroEL chaperonin at two molecules per tetradecamer. Applying this approach to the holin and antiholin proteins of phage λ, we find that both proteins are ...
John, Deaton +5 more
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Functional Analysis of a Class I Holin, P2 Y [PDF]
Journal of Bacteriology, 2013 Y is the putative holin gene of the paradigm coliphage P2 and encodes a 93-amino-acid protein. Y is predicted to be an integral membrane protein that adopts an N-out C-in membrane topology with 3 transmembrane domains (TMDs) and a highly charged C-terminal cytoplasmic tail.
Kam H, To +4 more
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, 2022 A cyanophage PaV-LD, previously isolated from harmful filamentous cyanobacterium Planktothrix agardhii, was sequenced, and co-expression of its two ORFs in tandem, ORF123 and ORF124, inhibited growth on the model cyanobacterium Synechocystis sp.
Qi-Ya Zhang (311840) +3 more
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Holin of bacteriophage lambda: structural insights into a membrane lesion [PDF]
Molecular Microbiology, 2008 SummaryThe length of the infection cycle of double‐stranded DNA bacteriophages is controlled by phage‐encoded small integral membrane proteins, holins. Holins are the gatekeepers of the lysis process, possessing an intriguing ability to be triggered, at a precise time point, to form large holes in the cytoplasmic membrane of phage‐infected bacteria ...
Krupovic, Mart, Bamford, Dennis H.
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mSphere, 2017 Huanglongbing (HLB) is a severe disease of citrus caused by an uncultured alphaproteobacterium “Candidatus Liberibacter asiaticus” and transmitted by Asian citrus psyllids (Diaphorina citri).
Mukesh Jain +2 more
doaj +1 more source
PLoS ONE, 2022 Holins are bacteriophage-encoded transmembrane proteins that function to control the timing of bacterial lysis event, assist with the destabilization of the membrane proton motive force and in some models, generate large "pores" in the cell membrane to ...
Richard S Pollenz +2 more
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The holin of bacteriophage lambda forms rings with large diameter [PDF]
Molecular Microbiology, 2008 SummaryHolins control the length of the infection cycle of tailed phages (the Caudovirales) by oligomerizing to form lethal holes in the cytoplasmic membrane at a time dictated by their primary structure. Nothing is currently known about the physical basis of their oligomerization or the structure of the oligomers formed by any known holin. Here we use
Christos G, Savva +6 more
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Enzymes and Mechanisms Employed by Tailed Bacteriophages to Breach the Bacterial Cell Barriers
Viruses, 2018 Monoderm bacteria possess a cell envelope made of a cytoplasmic membrane and a cell wall, whereas diderm bacteria have and extra lipid layer, the outer membrane, covering the cell wall.
Sofia Fernandes, Carlos São-José
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Structural Studies of the Bacteriophage Lambda Holin S105 [PDF]
Microscopy and Microanalysis, 2006 Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3 ...
CK Savva +3 more
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