Results 51 to 60 of about 2,686 (200)
Purification and Biochemical Characterization of the Lambda Holin [PDF]
Journal of Bacteriology, 1998 ABSTRACT Holins are small phage-encoded cytoplasmic membrane proteins, remarkable for their ability to make membranes permeable in a temporally regulated manner. The purification of S105, the λ holin, and one of the two products of gene S is described.
D L, Smith +3 more
openaire +2 more sources
Mycobacteriophage D29 holin C‐terminal region functionally assists in holin aggregation and bacterial cell death [PDF]
The FEBS Journal, 2015 Holins are phage‐encoded small transmembrane proteins that perforate the bacterial cytoplasmic membrane. In most cases, this process allows the phage‐encoded peptidoglycan hydrolases to act on the cell wall, resulting in host cell lysis and phage release.
Soumya, Kamilla, Vikas, Jain
openaire +2 more sources
Phylogenetic relationships of the holins observed in lactococcal phages.
, 2022 We investigated the diversity of holins found in 253 complete lactococcal phage genomes available in GenBank. ClustalW (v2.1) was used to perform multiple alignments and generate a phylogenetic tree (S7 Data).
Xiaojun Zhu (352546) +4 more
core +1 more source
Oligohistidine Tag Mutagenesis of the λ Holin Gene [PDF]
Journal of Bacteriology, 1998 ABSTRACT Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage λ was overexpressed and the holin protein was purified to homogeneity by means ...
D L, Smith, R, Young
openaire +2 more sources
, 2022 Holins are small transmembrane proteins involved in the final stage of the lytic cycle of double-stranded DNA (dsDNA) phages. They cooperate with endolysins to achieve bacterial lysis, thereby releasing the phage progeny into the extracellular ...
Leprince, Audrey +9 more
core +1 more source
Frontiers in Microbiology, 2018 The pathogenicity locus (PaLoc) of Clostridioides difficile usually comprises five genes (tcdR, tcdB, tcdE, tcdA, tcdC). While the proteins TcdA and TcdB represent the main toxins of this pathogen, TcdR and TcdC are involved in the regulation of their ...
Denise Mehner-Breitfeld +8 more
doaj +1 more source
Mycobacteriophage endolysins: diverse and modular enzymes with multiple catalytic activities. [PDF]
PLoS ONE, 2012 The mycobacterial cell wall presents significant challenges to mycobacteriophages--viruses that infect mycobacterial hosts--because of its unusual structure containing a mycolic acid-rich mycobacterial outer membrane attached to an arabinogalactan layer ...
Kimberly M Payne, Graham F Hatfull
doaj +1 more source
Prediction of Phage Virion Proteins Using Machine Learning Methods
Molecules, 2023 Antimicrobial resistance (AMR) is a major problem and an immediate alternative to antibiotics is the need of the hour. Research on the possible alternative products to tackle bacterial infections is ongoing worldwide.
Ranjan Kumar Barman +2 more
doaj +1 more source
Reconstitution of Holin Activity with a Synthetic Peptide Containing the 1–32 Sequence Region of EJh, the EJ-1 Phage Holin [PDF]
Journal of Biological Chemistry, 2003 Pneumococcal EJ-1 phage holin (EJh) is a hydrophobic polypeptide of 85 amino acid residues displaying lethal inner membrane disruption activity. To get an insight into holin structure and function, several peptides representing the different topological regions predicted by sequence analysis have been synthesized.
Haro, Amparo +6 more
openaire +3 more sources
, 2022 P1 is a model temperate myovirus. It infects different Enterobacteriaceae and can develop lytically or form lysogens. Only some P1 adaptation strategies to propagate in different hosts are known.
Wioleta Izak +4 more
core +1 more source