Results 61 to 70 of about 2,686 (200)
Biochemical and Genetic Characterization of Bacteriophage Holins [PDF]
, 2014 Bacteriophages infect and kill bacterial cells. During the infection cycle, a phage attaches to the host cell surface, then ejects its DNA into the cytoplasm, where its progenies are subsequently assembled.
To, Kam Ho
core +1 more source
Functional Analysis of a Class I Holin, P2 Y [PDF]
Journal of Bacteriology, 2013 Y is the putative holin gene of the paradigm coliphage P2 and encodes a 93-amino-acid protein. Y is predicted to be an integral membrane protein that adopts an N-out C-in membrane topology with 3 transmembrane domains (TMDs) and a highly charged C-terminal cytoplasmic tail.
Kam H, To +4 more
openaire +2 more sources
Functional Analysis of the Holin-Like Proteins of Mycobacteriophage Ms6 [PDF]
Journal of Bacteriology, 2011 ABSTRACT The mycobacteriophage Ms6 is a temperate double-stranded DNA (dsDNA) bacteriophage which, in addition to the predicted endolysin (LysA)-holin (Gp4) lysis system, encodes three additional proteins within its lysis module: Gp1, LysB, and Gp5. Ms6 Gp4 was previously described as a class II holin-like protein. By analysis of
Maria João, Catalão +3 more
openaire +2 more sources
The holin of bacteriophage lambda forms rings with large diameter [PDF]
Molecular Microbiology, 2008 SummaryHolins control the length of the infection cycle of tailed phages (the Caudovirales) by oligomerizing to form lethal holes in the cytoplasmic membrane at a time dictated by their primary structure. Nothing is currently known about the physical basis of their oligomerization or the structure of the oligomers formed by any known holin. Here we use
Christos G, Savva +6 more
openaire +2 more sources
The Mycobacteriophage Ms6 LysB N-Terminus Displays Peptidoglycan Binding Affinity
Viruses, 2021 Double-stranded DNA bacteriophages end their lytic cycle by disrupting the host cell envelope, which allows the release of the virion progeny. Each phage must synthesize lysis proteins that target each cell barrier to phage release. In addition to holins,
Adriano M. Gigante +6 more
doaj +1 more source
Holin of bacteriophage lambda: structural insights into a membrane lesion [PDF]
Molecular Microbiology, 2008 SummaryThe length of the infection cycle of double‐stranded DNA bacteriophages is controlled by phage‐encoded small integral membrane proteins, holins. Holins are the gatekeepers of the lysis process, possessing an intriguing ability to be triggered, at a precise time point, to form large holes in the cytoplasmic membrane of phage‐infected bacteria ...
Krupovic, Mart, Bamford, Dennis H.
openaire +3 more sources
Sizing the Holin Lesion with an Endolysin-β-Galactosidase Fusion [PDF]
Journal of Bacteriology, 2003 ABSTRACT Double-stranded DNA phages require two proteins for efficient host lysis: the endolysin, a muralytic enzyme, and the holin, a small membrane protein. In an event that defines the end of the vegetative cycle, the λ holin S acts suddenly to permeabilize the membrane.
Ing-Nang, Wang, John, Deaton, Ry, Young
openaire +2 more sources
Solubilization and delivery by GroEL of megadalton complexes of the λ holin [PDF]
Protein Science, 2004 AbstractGroEL can solubilize membrane proteins by binding them in its hydrophobic cavity when detergent is removed by dialysis. The best‐studied example is bacteriorhodopsin, which can bind in the GroEL chaperonin at two molecules per tetradecamer. Applying this approach to the holin and antiholin proteins of phage λ, we find that both proteins are ...
John, Deaton +5 more
openaire +2 more sources
Supplementary Material for: Analysis of 58 Families of Holins Using a Novel Program, PhyST
, 2016 We have designed a freely accessible program, PhyST, which allows the automated characterization of any family of homologous proteins within the Transporter Classification Database.
Moreno-Hagelsieb G. (3069615) +3 more
core +1 more source
Structural Studies of the Bacteriophage Lambda Holin S105 [PDF]
Microscopy and Microanalysis, 2006 Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3 ...
CK Savva +3 more
openaire +1 more source