Results 131 to 140 of about 11,515,911 (324)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Fluorescent protein-expressing Modified Vaccinia Ankara encoding T7 RNA polymerase

Journal of Virology
Efficient recovery of negative-stranded RNA viruses from plasmid cDNA requires robust bacteriophage T7 RNA polymerase expression, commonly supplied by Modified Vaccinia Ankara expressing T7 polymerase (MVA T7).
Nathaniel Jackson, Mahmoud Bayoumi, Himadri Nath, Luis Martinez-Sobrido   +3 more
doaj   +1 more source

Within-Host Dynamics of Multi-Species Infections:Facilitation, Competition and Virulence [PDF]

, 2012
Host individuals are often infected with more than one parasite species (parasites defined broadly, to include viruses and bacteria). Yet, research in infection biology is dominated by studies on single-parasite infections.
Eswarappa, Sandeepa M., Sylvie Estrela, Sam P Brown, Sylvie Estrela (156401), Estrela, Sylvie, Sam P. Brown (30600), Brown, Sam P., Sandeepa M Eswarappa, Sandeepa M. Eswarappa (156396), Brown Sam P., Estrela Sylvie, Eswarappa Sandeepa M.   +11 more
core   +2 more sources

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

A brief overview of mathematical modeling of the within-host dynamics of Mycobacterium tuberculosis

Frontiers in Applied Mathematics and Statistics
Tuberculosis (TB), a disease caused by bacteria Mycobacterium tuberculosis (Mtb), remains one of the major infectious diseases of humans with 10 million TB cases and 1.5 million deaths due to TB worldwide yearly.
Dipanjan Chakraborty, Saikat Batabyal, Vitaly V. Ganusov, Vitaly V. Ganusov   +3 more
doaj   +1 more source

Revealing host-pathogen interactions via receptor-binding using tissue microarrays

, 2023
The binding of a viral pathogen to the host cell is the first, crucial, step of infection. This first step in host-pathogen interactions is dependent on the attachment tools of the viral pathogen and the presentation of attachment factors by the host ...
Nemanichvili, Nikoloz, Verheije, Hélène, Faculteit Diergeneeskunde, Gröne, Andrea, de Vries, Robert   +4 more
core  

Technologies for Proteome-Wide Discovery of Extracellular Host-Pathogen Interactions [PDF]

, 2017
Pathogens have evolved unique mechanisms to breach the cell surface barrier and manipulate the host immune response to establish a productive infection. Proteins exposed to the extracellular environment, both cell surface-expressed receptors and secreted
Nadia Martinez-Martin
core   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Development and characterization of mouse-adapted recombinant SARS-CoV-2 expressing reporter genes

Microbiology Spectrum
SARS-CoV-2 is the causative agent of COVID-19. The ancestral SARS-CoV-2 Washington-1 (WA1) strain does not infect standard laboratory mouse strains, necessitating the use of mouse-adapted (MA) viruses.
Sara H. Mahmoud, Nathaniel Jackson, Ramya S. Barre, Yao Ma, Mahmoud Bayoumi, Esteban M. Castro, Shahrzad Ezzatpour, Richard K. Plemper, Stanley Perlman, Chengjin Ye, Luis Martinez-Sobrido   +10 more
doaj   +1 more source

Characterization of a Toxoplasma effector uncovers an alternative GSK3/β-catenin-regulatory pathway of inflammation

eLife, 2018
The intracellular parasite Toxoplasma gondii, hijacks evolutionarily conserved host processes by delivering effector proteins into the host cell that shift gene expression in a timely fashion.
Huan He, Marie-Pierre Brenier-Pinchart, Laurence Braun, Alexandra Kraut, Bastien Touquet, Yohann Couté, Isabelle Tardieux, Mohamed-Ali Hakimi, Alexandre Bougdour   +8 more
doaj   +1 more source