Results 41 to 50 of about 102,593 (165)

DNA Methylation and Differentiation of Human Keratinocytes

Journal of Investigative Dermatology, 1989
DNA methylation is a postreplicative modification thought to play a role in gene transcription in eucaryotes. Differences in the amount of 5-methylcytosine as a function of age and differentiation state have been reported. DNA isolated from human skin keratinocytes was analyzed for its 5-methylcytosine content. The 5-methylcytosine in DNA from neonatal
Veres, Denise A., Wilkins, Leon, Coble, Don W., Lyon, Sue B.   +3 more
openaire   +2 more sources

Thermolysin in human cultured keratinocyte isolation [PDF]

Brazilian Journal of Biology, 2007
BACKGROUND: When treating extensively burned patients using cultured epidermal sheets, the main problem is the time required for its production. Conventional keratinocyte isolation is usually done using Trypsin. We used a modification of the conventional isolation method in order to improve this process and increase the number of colonies from the ...
Gragnani, A., Sobral, CS., Ferreira, LM.
openaire   +5 more sources

Glycofullerenes Inhibit Particulate Matter Induced Inflammation and Loss of Barrier Proteins in HaCaT Human Keratinocytes

Biomolecules, 2020
Exposure to particulate matter (PM) has been linked to pulmonary and cardiovascular dysfunctions, as well as skin diseases, etc. PM impairs the skin barrier functions and is also involved in the initiation or exacerbation of skin inflammation, which is ...
Chiang-Wen Lee, Yu-Han Su, Yao-Chang Chiang, I-Ta Lee, Sin-Yu Li, Hui-Chun Lee, Lee-Fen Hsu, Yi-Ling Yan, Hsing-Yen Li, Ming-Chun Chen, Kuo-Ti Peng, Chian-Hui Lai   +11 more
doaj   +1 more source

Evaluation of Wound Healing and Antibacterial Potential of Greyia radlkoferi Szyszyl. Ethanolic Leaf Extract

Frontiers in Pharmacology, 2022
Angiogenesis is an essential mechanism in both physiological and pathological functions, such as wound healing and cancer metastasis. Several growth factors mediate angiogenesis, including vascular endothelial growth factor (VEGF) and platelet derived ...
Samantha Rae Loggenberg, Danielle Twilley, Marco Nuno De Canha, Debra Meyer, Ephraim Cebisa Mabena, Namrita Lall, Namrita Lall, Namrita Lall, Namrita Lall   +8 more
doaj   +1 more source

Protective effect of silk lutein on ultraviolet B-irradiated human keratinocytes

Biological Research, 2013
Carotenoids are efficient antioxidants that are of great importance for human health. Lutein and zeaxanthin are carotinoids present in high concentrations in the human retina which are involved in the photoprotection of the human eye.
Sutatip Pongcharoen, Prateep Warnnissorn, Ongart Lertkajornsin, Nanteetip Limpeanchob, Manote Sutheerawattananonda   +4 more
doaj  

Protective Potential of a Botanical-Based Supplement Ingredient against the Impact of Environmental Pollution on Cutaneous and Cardiopulmonary Systems: Preclinical Study

Current Issues in Molecular Biology
Air pollution is a growing threat to human health. Airborne pollution effects on respiratory, cardiovascular and skin health are well-established. The main mechanisms of air-pollution-induced health effects involve oxidative stress and inflammation.
Laurent Peno-Mazzarino, Nikita Radionov, Marián Merino, Sonia González, José L. Mullor, Jonathan Jones, Nuria Caturla   +6 more
doaj   +1 more source

Bio-Guided Targeting for Preservative and Anti-Ageing Cosmetic Ingredient Development

Cosmetics, 2014
To develop a new antioxidant, antibacterial and natural cosmetic ingredient without cytotoxicity to skin cells, bioactive molecules contained in Kalanchoe pinnata leaf methanolic extract were targeted using semi-preparative HPLC fractionation linked to ...
Emilie Destandau, Isabelle Krolikiewicz-Renimel, Saida El Abdellaoui, Perrine Cancellieri, Laetitia Fougère, Alix Toribio, Ludovic Landemarre, Patrice André, Claire Elfakir   +8 more
doaj   +1 more source

Cultured Human Keratinocytes Express Tropoelastin

Journal of Investigative Dermatology, 1997
We detected elastin mRNA in cultured normal human keratinocytes by RNase protection assay. The content of elastin mRNA was estimated at approximately one-twentieth of that of cultured skin fibroblasts. Tropoelastin polypeptide with a molecular weight of 68 kDa was detected in the preparation of culture medium of normal human keratinocytes by western ...
Kajiya, Hiroyoshi, Tanaka, Nobuhiko, Inazumi, Toyoko, Seyama, Yoshiyuki, Tajima, Shingo, Ishibashi, Akira   +5 more
openaire   +2 more sources

Interleukin-38 interacts with destrin/actin-depolymerizing factor in human keratinocytes.

PLoS ONE, 2019
Interleukin (IL)-38 is a member of the IL-1 family of cytokines, which was proposed to exert anti-inflammatory effects. IL-38 is constitutively expressed in the skin, where keratinocytes are the main producing cells.
Dominique Talabot-Ayer, Loïc Mermoud, Julia Borowczyk, Justyna Drukala, Michal Wolnicki, Ali Modarressi, Wolf-Henning Boehncke, Nicolo Brembilla, Gaby Palmer   +8 more
doaj   +1 more source

Efficient CRISPR-Cas9-Mediated Gene Ablation in Human Keratinocytes to Recapitulate Genodermatoses: Modeling of Netherton Syndrome

Molecular Therapy: Methods & Clinical Development, 2020
Current efforts to find specific genodermatoses treatments and define precise pathogenesis mechanisms require appropriate surrogate models with human cells.
Victoria Gálvez, Esteban Chacón-Solano, Jose Bonafont, Ángeles Mencía, Wei-Li Di, Rodolfo Murillas, Sara Llames, Asunción Vicente, Marcela Del Rio, Marta Carretero, Fernando Larcher   +10 more
doaj   +1 more source