Results 31 to 40 of about 119,569 (265)
SAGE Open Medicine, 2020 Objectives: To establish a straightforward single-cell passaging cultivation method that enables high-quality maintenance of human induced pluripotent stem cells without the appearance of karyotypic abnormalities or loss of pluripotency.
Estela Cruvinel +14 more
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Stem Cell ResearchThe role of the nuclear thyroid hormone receptor TRα1 as a mediator of thyroid hormone action on target gene expression is well understood. However, the function of the TRα2 splicing isoform, which does not bind thyroid hormones, remains unexplored.
Nina Härting +4 more
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A novel -mutated human induced pluripotent stem cell model for understanding -mutated tumors
Tumor Biology, 2020 A missense mutation of the guanine nucleotide binding protein alpha stimulating activity polypeptide 1 ( GNAS ) gene, typically Arg201Cys or Arg201His (R201H/R201C), leads to constitutive activation of the Gsα-cyclic AMP (cAMP) signaling pathway that ...
Katsuhito Watanabe +5 more
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Heart regeneration with human pluripotent stem cells: Prospects and challenges
Bioactive Materials, 2020 Cardiovascular disease, ranging from congenital heart disease to adult myocardial infarction, is the leading cause of death worldwide. In pursuit of reliable cardiovascular regenerative medicine, human pluripotent stem cells (hPSCs), including human ...
Yuqian Jiang, Xiaojun Lance Lian
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Defining differentially methylated regions specific for the acquisition of pluripotency and maintenance in human pluripotent stem cells via microarray. [PDF]
PLoS ONE, 2014 Epigenetic regulation is critical for the maintenance of human pluripotent stem cells. It has been shown that pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem cells, appear to have a hypermethylated status compared with ...
WenYin He +9 more
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Genome Editing in Human Pluripotent Stem Cells [PDF]
, 2017 Genome editing in human pluripotent stem cells (hPSCs) enables the generation of reporter lines and knockout cell lines. Zinc finger nucleases, transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9 technology have recently increased the efficiency of proper gene editing by creating double strand breaks (DSB) at defined sequences in ...
Jared, Carlson-Stevermer, Krishanu, Saha
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Mitochondria in human pluripotent stem cell apoptosis [PDF]
Seminars in Cell & Developmental Biology, 2016 Human pluripotent stem cells (hPSCs) have great potential in regenerative medicine because they can differentiate into any cell type in the body. Genome integrity is vital for human development and for high fidelity passage of genetic information across generations through the germ line.
TeSlaa, Tara +2 more
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Epigenetic aberrations in human pluripotent stem cells [PDF]
The EMBO Journal, 2019 Human pluripotent stem cells (hPSCs) are being increasingly utilized worldwide in investigating human development, and modeling and discovering therapies for a wide range of diseases as well as a source for cellular therapy. Yet, since the first isolation of human embryonic stem cells (hESCs) 20 years ago, followed by the successful reprogramming of ...
Shiran Bar, Nissim Benvenisty
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Prevention of tumor risk associated with the reprogramming of human pluripotent stem cells
Journal of Experimental & Clinical Cancer Research, 2020 Human pluripotent embryonic stem cells have two special features: self-renewal and pluripotency. It is important to understand the properties of pluripotent stem cells and reprogrammed stem cells.
Kenly Wuputra +5 more
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Modelling Human Disease with Pluripotent Stem Cells [PDF]
Current Gene Therapy, 2013 Recent progress in the field of cellular reprogramming has opened up the doors to a new era of disease modelling, as pluripotent stem cells representing a myriad of genetic diseases can now be produced from patient tissue. These cells can be expanded and differentiated to produce a potentially limitless supply of the affected cell type, which can then ...
Richard, Siller +3 more
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