The deubiquitinating enzymes USP4 and USP17 target hyaluronan synthase 2 and differentially affect its function. [PDF]
Oncogenesis, 2017 The levels of hyaluronan, a ubiquitous glycosaminoglycan prominent in the extracellular matrix, is balanced through the actions of hyaluronan-synthesizing enzymes (HAS1, 2 and 3) and degrading hyaluronidases (Hyal 1, 2, 3 and PH20).
Mehić M +4 more
europepmc +2 more sources
Shear stress increases endothelial hyaluronan synthase 2 and hyaluronan synthesis especially in regard to an atheroprotective flow profile [PDF]
Experimental physiology, 2011 Recent studies revealed that in vivo the inner blood vessel surface is lined with an endothelial surface layer at least 0.5 mu m thick, which serves as an aegis, protecting the vessel wall from arteriosclerosis.
Fiedorowicz, Katarzyna +15 more
core +4 more sources
Quantitative continuous assay for hyaluronan synthase
Analytical Biochemistry, 2007 A rapid, continuous, and convenient three-enzyme coupled UV absorption assay was developed to quantitate the glucuronic acid and N-acetylglucosamine transferase activities of hyaluronan synthase from Pasteurella multocida (PmHAS).
Joanne C Krupa +2 more
exaly +2 more sources
Requirement of Hyaluronan Synthase-2 in Craniofacial and Palate Development. [PDF]
J Dent Res, 2019 Cleft palate is a common major birth defect resulting from disruption of palatal shelf growth, elevation, or fusion during fetal palatogenesis. Whereas the molecular mechanism controlling palatal shelf elevation is not well understood, a prevailing hypothesis is that region-specific accumulation of hyaluronan, a predominant ...
Lan Y, Qin C, Jiang R.
europepmc +4 more sources
Hyaluronan synthase 2 (HAS2) overexpression diminishes the procatabolic activity of chondrocytes by a mechanism independent of extracellular hyaluronan. [PDF]
J Biol Chem, 2019 Osteoarthritis (OA) is a progressive degenerative disease of the joints caused in part by a change in the phenotype of resident chondrocytes within affected joints. This altered phenotype, often termed proinflammatory or procatabolic, features enhanced production of endoproteinases and matrix metallo-proteinases (MMPs) as well as secretion of ...
Ishizuka S +7 more
europepmc +4 more sources
Hyaluronan synthase 2 regulates fibroblast senescence in pulmonary fibrosis. [PDF]
Matrix Biol, 2016 Dysregulated repair of lung injury often results in lung fibrosis characterized by unremitting deposition of matrix components including glycosaminoglycan hyaluronan (HA). HA is mainly produced by hyaluronan synthases (HAS) in mesenchymal cells.
Li Y +9 more
europepmc +3 more sources
Synaptic Hyaluronan Synthesis and CD44-Mediated Signaling Coordinate Neural Circuit Development
Cells, 2021 The hyaluronan-based extracellular matrix is expressed throughout nervous system development and is well-known for the formation of perineuronal nets around inhibitory interneurons.
Emily S. Wilson, Karen Litwa
doaj +1 more source
Human Keratinocytes Respond to Extracellular UTP by Induction of Hyaluronan Synthase 2 Expression and Increased Hyaluronan Synthesis. [PDF]
J Biol Chem, 2017 The release of nucleotides into extracellular space is triggered by insults like wounding and ultraviolet radiation, resulting in stimulatory or inhibitory signals via plasma membrane nucleotide receptors. As similar insults are known to activate hyaluronan synthesis we explored the possibility that extracellular UTP or its breakdown products UDP and ...
Jokela T +7 more
europepmc +4 more sources
The activity of hyaluronan synthase 2 is regulated by dimerization and ubiquitination. [PDF]
J Biol Chem, 2010 Hyaluronan is a component of the extracellular matrix, which affects tissue homeostasis. In this study, we investigated the regulatory mechanisms of one of the hyaluronan-synthesizing enzymes, HAS2. Ectopic expression of Flag- and 6myc-HAS2 in COS-1 cells followed by immunoprecipitation and immunoblotting revealed homodimers; after co-transfection with
Karousou E +9 more
europepmc +5 more sources
Functional analysis of human hyaluronan synthase 3 splicing variant 2
, 2010 Hyaluronan is a large glycosaminoglycan mainly presented in the extracellular matrix as well as in the intracellular compartment. Hyaluronan is synthesized by three hyaluronan synthase isoforms, namely HAS1, HAS2 and HAS3.
Dai, Guang
core +3 more sources