Results 121 to 130 of about 23,113 (239)

Optimization of electrofusion parameters for producing hybridomas synthesizing human monoclonal antibodies

open access: yes, 2021
Monoclonal antibodies are an established treatment for many diseases, including cancer, toxic conditions and infectious diseases. The goal of this work was to optimize the conditions for obtaining hybridomas secreting human monoclonal IgG antibodies.
Silkina M. V.
core  

A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies.

open access: yes, 1988
A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant ...
Chervonsky, A V   +5 more
core   +1 more source

Electrochemical and Biological Evaluation of Patterned TiO2@TiC‐Nanowires for Bio‐Interface Electrode

open access: yesSmall, Volume 22, Issue 61, 2 November 2026.
This study introduces a room‐temperature process to fabricate patterned TiO2@TiC core–shell nanowires, merging the biocompatibility of TiO2‐NWs with the high conductivity of TiC. Through sputtering pressure and time modulation, the nanowires morphology and electrochemical properties are tuned, enabling enhanced capacitance, stability, and guided ...
Roaa Sait   +16 more
wiley   +1 more source

Induction of long-term but reversible unresponsiveness after activation of murine T cell hybridomas.

open access: yes, 1991
T cell receptor (TCR)-mediated stimulation of murine T cell hybridomas induces cell activation and lymphokine production. We have observed that following productive activation, T cell hybridomas become refractory to a subsequent stimulation ...
Kaufman, Marcelle   +6 more
core   +1 more source

Double‐Antibody Sandwich Enzyme‐Linked Immunosorbent Assay for Detecting Feline Coronavirus Infection

open access: yesVeterinary Medicine and Science, Volume 12, Issue 6, November 2026.
In this study, a mouse monoclonal and rabbit polyclonal antibodies were used as capture and detection antibodies, respectively, to develop a DAS‐ELISA that can be used for viral antigen detection. ABSTRACT Objectives Double‐antibody sandwich enzyme‐linked immunosorbent assay (DAS‐ELISA) is a simple and accurate method for detecting viral pathogens ...
Bo Dong   +7 more
wiley   +1 more source

Peripheral T cells poly-recognize gut bacterial species.

open access: yes, 2018
T cell hybridomas were generated from C57BL/6J mice monoassociated with B. thetaiotaomicron VPI 5482 after in vitro stimulation with heat-killed bacteria.
Daniel A. Peterson (146145)   +4 more
core   +1 more source

The E‐cadherin‐Wnt‐mir‐994 Axis Repurposes a Cadherin Switch for Niche Robustness and Germline Stem Cell Maintenance

open access: yesCell Proliferation, Volume 59, Issue 11, November 2026.
In the Drosophila ovarian niche, an E‐cadherin‐to‐N‐cadherin switch, mediated by Wnt‐mir‐994 signalling, is repurposed to ensure niche resilience. This compensatory mechanism maintains niche integrity and stem cell support upon E‐cadherin loss, revealing a robustness circuit.
Renjun Tu   +6 more
wiley   +1 more source

Construction of Rat-Mouse T Cell Hybridomas That Express the Rat Interleukin 2 Receptors and Analysis of Mechanisms Involved in the Regulation of Their Expression [PDF]

open access: yes
Spleen cells obtained from Lewis rats were cultured with 4μg/ml Con A for 96 hr and the activated cells were fused with BW5147, mouse T lymphoma cells. Three clones were obtained by fusion and expressed the rat interleukin 2 receptor (IL-2R).
上出, 利光, 幸田, 弘信
core   +1 more source

Myo1g Mediates NKR‐P1C Expression in Primary Mouse NK Cells and Participates in Cytotoxicity

open access: yesImmunology, Volume 179, Issue 3, Page 516-524, November 2026.
In this study we analysed the participation of Myosin 1g in the development and functions of murine natural killer (NK) cells; we found that Myosin 1g is expressed in murine‐activated primary NK cells from the spleen. Additionally, in the absence of Myosin 1g, correlates with a reduced expression of NKR‐P1C but not of CD49b or NKp46.
Héctor Romero‐Ramírez   +3 more
wiley   +1 more source

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