Results 91 to 100 of about 139,474 (279)

Towards genomic medicine: a tailored next-generation sequencing panel for hydroxyurea pharmacogenomics in Tanzania

BMC Medical Genomics
Background Pharmacogenomics of hydroxyurea is an important aspect in the management of sickle cell disease (SCD), especially in the era of genomic medicine.
Siana Nkya, Collin Nzunda, Emmanuel Saukiwa, Frida Kaywanga, Eliud Buberwa, David Solomon, Heavenlight Christopher, Doreen Ngowi, Julieth Johansen, Florence Urio, Josephine Mgaya, Salman Karim, Mohamed Zahir Alimohamed, Raphael Z. Sangeda, Clara Chamba, Emile R. Chimusa, Enrico Novelli, Julie Makani   +17 more
doaj   +1 more source

Assessing the Gene Content of the Megagenome: Sugar Pine (Pinus lambertiana). [PDF]

, 2016
Sugar pine (Pinus lambertiana Douglas) is within the subgenus Strobus with an estimated genome size of 31 Gbp. Transcriptomic resources are of particular interest in conifers due to the challenges presented in their megagenomes for gene identification ...
Delfino-Mix, Annette, Famula, Randi A, Gonzalez-Ibeas, Daniel, Langley, Charles H, Loopstra, Carol A, Martinez-Garcia, Pedro J, Neale, David B, Stevens, Kristian A, Wegrzyn, Jill L   +8 more
core   +2 more sources

16S Sequencing on Illumina MiSeq Platform v1

The 16S protocol detailed here is designed to amplify bacteria using V4-V5 hyper-variable regions of the 16S rRNA gene on the Illumina MiSeq DNA sequencer.
Angela Schmoldt, Melissa Schussman
openaire   +1 more source

Preparing multiplexed 16S/18S/ITS amplicons for the Illumina MiSeq v1

, 2022
The following detailed protocol is for the generation of paired-end sequencing reads of 16S/18S/ITS PCR amplicons with dual barcodes (i.e.: “indices”) on the Illumina MiSeq machine of length ≈450 bp (ie: 300+300 bp sequencing with ~150 bp overlap) using v3 600 cycle chemistry. It assumes an input of 380 individual samples (+4 controls = 384) in four 96-
André M Comeau, Alessi Kwawukume
openaire   +1 more source

Mapping the subgingival HerBiome and HisBiome over the human healthspan

Journal of Periodontology, EarlyView.
Abstract Background Understanding the intricate relationship between sex, age, and the oral microbiome is crucial for deciphering the onset and progression of numerous age‐related oral and systemic diseases. Methods Subgingival plaque was collected from 781 periodontally and systemically healthy females and 160 males spanning 0 to 80 years.
Rahul Nikam, Kazune Pax, Michelle Lee‐Scott Beverly, Purnima S. Kumar   +3 more
wiley   +1 more source

Rabies Virus Sequencing using Illumina- MiSeq v1

This Rabies whole genome sequencing protocol has been derived and modified from the Illumina COVIDSeq RUO sequencing pipeline. The protocol has been modified and optimised for sequencing Rabies virus (RABV). The methodology uses RABV-specific primers that have been designed in-house.
Chakrakodi N Varun, Dhanya K, Ashwini M Ananda, Reeta Mani   +3 more
openaire   +1 more source

Comparative analysis of fungal microbiomes in Korean and Chinese chili pepper powders

Journal of the Science of Food and Agriculture, EarlyView.
Abstract BACKGROUND Chili pepper powder plays a crucial role in kimchi fermentation; however, its use may result in fungal contamination. Therefore, analyzing its mycobiome is vital to maintain quality control standards. In this study, we used internal transcribed spacer (ITS)2 amplicon sequencing to compare fungal communities in commercially available
Jin‐Hee Seo, Ji‐Hyoung Ha, Su‐Hyeon Cho, Kil‐Nam Kim, Hae‐Won Lee   +4 more
wiley   +1 more source

Whole Genome Sequencing of Influenza A and B Viruses With the MinION Sequencer in the Clinical Setting: A Pilot Study

Frontiers in Microbiology, 2018
Introduction: Whole genome sequencing (WGS) of influenza viruses is important for preparing vaccines and coping with newly emerging viruses. However, WGS is difficult to perform using conventional next-generation sequencers in developing countries, where
Kazuo Imai, Kazuo Imai, Kazuo Imai, Kaku Tamura, Tomomi Tanigaki, Mari Takizawa, Eiko Nakayama, Takahiko Taniguchi, Misako Okamoto, Yasumasa Nishiyama, Norihito Tarumoto, Norihito Tarumoto, Kotaro Mitsutake, Kotaro Mitsutake, Takashi Murakami, Takashi Murakami, Shigefumi Maesaki, Shigefumi Maesaki, Takuya Maeda, Takuya Maeda   +19 more
doaj   +1 more source

Biochar‐Induced Shifts in Fungal Community Structure and Their Association With Soil Physical Properties in Degraded Soil From the Brazilian Semiarid

Land Degradation &Development, EarlyView.
ABSTRACT Soil degradation compromises ecosystem functioning. Biochar, a carbon‐rich amendment, has gained attention as a promising strategy to enhance soil structure and restore microbial balance. This study investigated the effects of two biochars, cashew bagasse biochar (CBB) and a co‐pyrolyzed biochar produced from sewage sludge and cashew pruning ...
João Marcos Rodrigues dos Santos, Arthur Prudêncio de Araujo Pereira, Kaio Gráculo Vieira Garcia, Ícaro Vasconcelos do Nascimento, Francisco Luan Almeida Barbosa, Tiago da Costa Dantas Moniz, Gilvanete da Silva Henrique, Murilo de Sousa Almeida, Vania Maria Maciel Melo, Mirian Cristina Gomes Costa, Helon Hébano de Freitas Sousa, Adriana Guirado Artur, Christiana de Fátima Bruce da Silva, Odair Pastor Ferreira, Antônio Gomes de Souza Filho, Erika Valente de Medeiros, Ademir Sergio Ferreira Araujo, Rafael Santiago da Costa, Jaedson Cláudio Anunciato Mota   +18 more
wiley   +1 more source

Sediment trap preservative choice impacts particle‐associated microbial community composition

Limnology and Oceanography: Methods, EarlyView.
Abstract Understanding and quantifying the microbial contribution to particulate organic matter (POM) flux and carbon cycling in the ocean relies largely on the collection of sinking POM. A critical aspect of evaluating microbial communities on sediment trap‐collected POM is their preservation until recovery and sample processing.
Nicola L. Paul, Colleen A. Durkin, Sebastian Sudek, Matthieu Bressac, Philip W. Boyd, Eric J. Tappa, Claudia R. Benitez‐Nelson, Alyson E. Santoro   +7 more
wiley   +1 more source