Results 91 to 100 of about 142,852 (247)

Towards genomic medicine: a tailored next-generation sequencing panel for hydroxyurea pharmacogenomics in Tanzania

BMC Medical Genomics
Background Pharmacogenomics of hydroxyurea is an important aspect in the management of sickle cell disease (SCD), especially in the era of genomic medicine.
Siana Nkya, Collin Nzunda, Emmanuel Saukiwa, Frida Kaywanga, Eliud Buberwa, David Solomon, Heavenlight Christopher, Doreen Ngowi, Julieth Johansen, Florence Urio, Josephine Mgaya, Salman Karim, Mohamed Zahir Alimohamed, Raphael Z. Sangeda, Clara Chamba, Emile R. Chimusa, Enrico Novelli, Julie Makani   +17 more
doaj   +1 more source

16S Sequencing on Illumina MiSeq Platform v1

The 16S protocol detailed here is designed to amplify bacteria using V4-V5 hyper-variable regions of the 16S rRNA gene on the Illumina MiSeq DNA sequencer.
Angela Schmoldt, Melissa Schussman
openaire   +1 more source

Preparing multiplexed 16S/18S/ITS amplicons for the Illumina MiSeq v1

, 2022
The following detailed protocol is for the generation of paired-end sequencing reads of 16S/18S/ITS PCR amplicons with dual barcodes (i.e.: “indices”) on the Illumina MiSeq machine of length ≈450 bp (ie: 300+300 bp sequencing with ~150 bp overlap) using v3 600 cycle chemistry. It assumes an input of 380 individual samples (+4 controls = 384) in four 96-
André M Comeau, Alessi Kwawukume
openaire   +1 more source

Whole Genome Sequencing of Influenza A and B Viruses With the MinION Sequencer in the Clinical Setting: A Pilot Study

Frontiers in Microbiology, 2018
Introduction: Whole genome sequencing (WGS) of influenza viruses is important for preparing vaccines and coping with newly emerging viruses. However, WGS is difficult to perform using conventional next-generation sequencers in developing countries, where
Kazuo Imai, Kazuo Imai, Kazuo Imai, Kaku Tamura, Tomomi Tanigaki, Mari Takizawa, Eiko Nakayama, Takahiko Taniguchi, Misako Okamoto, Yasumasa Nishiyama, Norihito Tarumoto, Norihito Tarumoto, Kotaro Mitsutake, Kotaro Mitsutake, Takashi Murakami, Takashi Murakami, Shigefumi Maesaki, Shigefumi Maesaki, Takuya Maeda, Takuya Maeda   +19 more
doaj   +1 more source

Rabies Virus Sequencing using Illumina- MiSeq v1

This Rabies whole genome sequencing protocol has been derived and modified from the Illumina COVIDSeq RUO sequencing pipeline. The protocol has been modified and optimised for sequencing Rabies virus (RABV). The methodology uses RABV-specific primers that have been designed in-house.
Chakrakodi N Varun, Dhanya K, Ashwini M Ananda, Reeta Mani   +3 more
openaire   +1 more source

The influence of ultra‐processed foods on gut microbiome and inflammatory markers in schoolchildren from Northeastern Brazil

Journal of Pediatric Gastroenterology and Nutrition, EarlyView.
Abstract Objective This study investigated the relationship between the consumption of ultra‐processed foods (UPF), dietary profile, and inflammation on the intestinal microbiome in children. Methods A cross‐sectional study was conducted using data from a community‐based controlled trial involving 82 children aged 7–11 years enrolled in public schools ...
Cristiane Cosmo Silva‐Luis, Paulo César Trindade da Costa, Vinicius José Baccin Martins, José Luiz de Brito Alves   +3 more
wiley   +1 more source

Improved COI taxonomic assignment workflow enhances detection of invertebrates in environmental DNA

Limnology and Oceanography: Methods, EarlyView.
Abstract Environmental DNA (eDNA) metabarcoding is an increasingly popular and powerful tool in ecology, offering a non‐invasive method to assess biodiversity and monitor ecosystem health across diverse environments. While the mitochondrial cytochrome c oxidase subunit I (COI) gene is one of the most widely used markers for studying eukaryotic ...
Yuanyu Cheng, David Walsh, Joanna Gauthier, Marie‐Eve Monchamp, Daniel T. Selbie, Irene Gregory‐Eaves   +5 more
wiley   +1 more source

Sediment trap preservative choice impacts particle‐associated microbial community composition

Limnology and Oceanography: Methods, EarlyView.
Abstract Understanding and quantifying the microbial contribution to particulate organic matter (POM) flux and carbon cycling in the ocean relies largely on the collection of sinking POM. A critical aspect of evaluating microbial communities on sediment trap‐collected POM is their preservation until recovery and sample processing.
Nicola L. Paul, Colleen A. Durkin, Sebastian Sudek, Matthieu Bressac, Philip W. Boyd, Eric J. Tappa, Claudia R. Benitez‐Nelson, Alyson E. Santoro   +7 more
wiley   +1 more source

Enhanced Qiaseq Direct SARS-CoV-2 kit for Illumina Miseq v1

, 2022
This SARS-CoV-2 targeted, and rapid viral RNA library preparation and sequencing kit provides an approximately five hour turn-around time from viral RNA sample to sequencing.The kit includes cDNA synthesis, enrichment, and library amplification and indexing.The enhanced protocol removes the need for normalization and quantification prior to ...
Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim   +4 more
openaire   +1 more source

A case of yogurt central line‐associated bloodstream infection in a child with intestinal failure

Nutrition in Clinical Practice, EarlyView.
Abstract Central line‐associated bloodstream infections (CLABSI) are a significant cause of morbidity in children with intestinal failure (IF). Probiotic therapy is discouraged in patients with IF because of the increased risk of bacteremia with the probiotic organism.
Nasiha Rahim, Emily Keller, Ellen Wagner, Andrea Berkemeyer, Timothy Johnson, Daniel Johnson, Timothy Sentongo   +6 more
wiley   +1 more source