Results 31 to 40 of about 139,474 (279)

EM-mosaic detects mosaic point mutations that contribute to congenital heart disease. [PDF]

, 2020
BackgroundThe contribution of somatic mosaicism, or genetic mutations arising after oocyte fertilization, to congenital heart disease (CHD) is not well understood.
Bernstein, Daniel, Brueckner, Martina, Chung, Wendy K, DePalma, Steven, Gelb, Bruce D, Goldmuntz, Elizabeth, Gorham, Joshua M, Griffin, Emily, Hsieh, Alexander, Kim, Richard W, Lifton, Richard P, Manheimer, Kathryn B, McKean, David, Morton, Sarah U, Newburger, Jane W, Porter, George A, Qi, Hongjian, Seidman, Christine E, Seidman, JG, Shen, Yufeng, Srivastava, Deepak, Tai, Angela C, Tristani-Firouzi, Martin, Willcox, Jon AL   +23 more
core   +3 more sources

Illumina iSeq 100 and MiSeq exhibit similar performance in freshwater fish environmental DNA metabarcoding

Scientific Reports, 2021
Environmental DNA (eDNA) analysis is a method of detecting DNA from environmental samples and is used as a biomonitoring tool. In recent studies, Illumina MiSeq has been the most extensively used tool for eDNA metabarcoding.
Ryohei Nakao, Ryutei Inui, Yoshihisa Akamatsu, Masuji Goto, Hideyuki Doi, Shunsuke Matsuoka   +5 more
doaj   +1 more source

16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]

, 2016
peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J., Clooney, Adam G, Cotter, Paul D., Fouhy, Fiona, STANTON, CATHERINE   +4 more
core   +2 more sources

Metagenomic investigation of faecal microbiota in sheep and goats of the same ages

Journal of Taibah University for Science, 2021
Despite the significant influence of the microbial communities that inhabit the ruminant’s gastrointestinal tract on their health and production, the study of the composition and variations in sheep and goats gut microbiome is limited.
I. I. Shabana, N. N. Albakri, N. A. Bouqellah   +2 more
doaj   +1 more source

Diversity of immunoglobulin light chain genes in non-teleost ray-finned fish uncovers IgL subdivision into five ancient isotypes [PDF]

, 2018
The aim of this study was to fill important gaps in the evolutionary history of immunoglobulins by examining the structure and diversity of IgL genes in non-teleost ray-finned fish.
Andreyushkova, Daria A, Baranov, Konstantin O, Chikaev, Nikolai A, Gessner, Jörn, Guselnikov, Sergey V, Kulemzin, Sergey V, Makunin, Alexey I, Mechetina, Ludmila V, Najakshin, Alexander M, Schartl, Manfred, Stöck, Matthias, Taranin, Alexander V, Trifonov, Vladimir A, Warren, Wesley C, Wuertz, Sven   +14 more
core   +5 more sources

cpn60 metagenomic amplicon library preparation for the Illumina Miseq platform

, 2021
Abstract This protocol can be applied to determine the composition of a microbial community. The cpn60 gene (also known as groEL, hsp60) is present in almost all bacteria and a 552-558 bp region of the gene has been established as a barcode for species level identification of bacteria. The primer cocktail used in this
Champika Fernando, Janet E. Hill
openaire   +1 more source

Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene. [PDF]

Sci Rep, 2016
AbstractGreen fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence.
Van den Hoecke S, Verhelst J, Saelens X.
europepmc   +5 more sources

Double-digest RADseq loci using standard Illumina indexes improve deep and shallow phylogenetic resolution of Lophodermium, a widespread fungal endophyte of pine needles. [PDF]

, 2018
The phylogenetic and population genetic structure of symbiotic microorganisms may correlate with important ecological traits that can be difficult to directly measure, such as host preferences or dispersal rates.
Oono, Ryoko, Salas-Lizana, Rodolfo
core   +1 more source

Diversity of cervical microbiota in asymptomatic chlamydia trachomatis genital infection: a pilot study [PDF]

, 2017
Chlamydia trachomatis genital infection continues to be an important public health problem worldwide due to its increasing incidence. C. trachomatis infection can lead to severe sequelae, such as pelvic inflammatory disease, obstructive infertility, and ...
DI PIETRO, Marisa, Farcomeni, Alessio, Filardo, Simone, Latino, Maria A, Porpora, Maria Grazia, Recine, Nadia, Sessa, Rosa   +6 more
core   +3 more sources

Phasing amplicon sequencing on Illumina Miseq for robust environmental microbial community analysis [PDF]

BMC Microbiology, 2015
Although high-throughput sequencing, such as Illumina-based technologies (e.g. MiSeq), has revolutionized microbial ecology, adaptation of amplicon sequencing for environmental microbial community analysis is challenging due to the problem of low base diversity.A new phasing amplicon sequencing approach (PAS) was developed by shifting sequencing phases
Wu, Liyou, Wen, Chongqing, Qin, Yujia, Yin, Huaqun, Tu, Qichao, Van Nostrand, Joy D., Yuan, Tong, Yuan, Menting, Deng, Ye, Zhou, Jizhong   +9 more
openaire   +2 more sources