Results 31 to 40 of about 142,852 (247)

Design and use of a new primer pair for the characterization of the cyanobacteria Synechococcus and Prochlorococcus communities targeting petB gene through metabarcoding approaches

open access: yesMethodsX, 2023
During the last years, the application of next-generation sequencing (NGS) technologies to search for specific genetic markers has become a crucial method for the characterization of microbial communities.
Alexandra Coello-Camba   +2 more
doaj   +1 more source

Effects of wheat root exudates on bacterial communities in the rhizosphere of watermelon

open access: yesPlant, Soil and Environment, 2021
In this study, we investigated the effects of wheat root exudates on soil bacterial communities in the watermelon rhizosphere using quantitative PCR and Illumina MiSeq sequencing.
Jibo Shi   +5 more
doaj   +1 more source

Comparative analysis of targeted next-generation sequencing for Plasmodium falciparum drug resistance markers

open access: yesScientific Reports, 2022
Well-defined molecular resistance markers are available for a range of antimalarial drugs, and molecular surveillance is increasingly important for monitoring antimalarial drug resistance.
Chanon Kunasol   +6 more
doaj   +1 more source

Reproducibility of methylated CpG typing with the Illumina MiSeq [PDF]

open access: yesForensic Science International: Genetics Supplement Series, 2017
Abstract DNA methylation patterns may be used for identification of body fluids and for age estimation of human individuals. We evaluated some of the challenges and pitfalls of studying methylated CpG sites. We compared the methylated CpG analysis of two different methods 1) massively parallel sequencing (MPS) using the Illumina MiSeq and 2) the iPLEX
Kampmann, Marie-Louise   +4 more
openaire   +2 more sources

Choosing a benchtop sequencing machine to characterise Helicobacter pylori genomes. [PDF]

open access: yesPLoS ONE, 2013
The fully annotated genome sequence of the European strain, 26695 was first published in 1997 and, in 1999, it was directly compared to the USA isolate J99, promoting two standard laboratory isolates for Helicobacter pylori (H. pylori) research. With the
Timothy T Perkins   +3 more
doaj   +1 more source

Analysis, optimization and verification of Illumina-generated 16S rRNA gene amplicon surveys. [PDF]

open access: yesPLoS ONE, 2014
The exploration of microbial communities by sequencing 16S rRNA genes has expanded with low-cost, high-throughput sequencing instruments. Illumina-based 16S rRNA gene sequencing has recently gained popularity over 454 pyrosequencing due to its lower ...
Michael C Nelson   +4 more
doaj   +1 more source

Illumina DNA Prep (M) Tagmentation Library Preparation for use on an Illumina MiSeq Sequencer v1 [PDF]

open access: yes, 2020
This procedure outlines the protocol for whole genome sequencing of bacterial organisms using the Illumina DNA Prep library preparation kit for sequencing on an Illumina MiSeq sequencer. This document applies to all laboratory personnel in the Division of Microbiology (DM) as well as laboratories in the GenomeTrakr Network.
Julie Haendiges   +3 more
openaire   +2 more sources

Sample preparation for Illumina MiSeq Dual Index metabarcoding v1 [PDF]

open access: yes, 2020
The protocol shows amplicon sequencing samples preoared by Illumina Dual Index strategy, and sequened with Illumina MiSeq (RRID:SCR_016379) for bacteria and microeukaryotes.
Xiaohuan Sun, Yuehua Hu, Zewei Song
openaire   +1 more source

A shared mucosal gut microbiota signature in primary sclerosing cholangitis before and after liver transplantation

open access: yesHepatology, EarlyView., 2022
In this study of the gut mucosal microbiota in primary sclerosing cholangitis (PSC), we found consistent microbiota features associated with PSC and recurrent PSC, PSC with inflammatory bowel disease and a persistent gut dysbiosis after liver transplantation Abstract Background and Aims Several characteristic features of the fecal microbiota have been ...
Mikal Jacob Hole   +12 more
wiley   +1 more source

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