Sample Preparation for Illumina MiSeq Dual Index Amplicon Sequencing v1 [PDF]
, 2019 Amplicon sequencing sample preparation for bacteria and microeukaryotes
Máté Vass, Anna Székely
openaire +2 more sources
Clinical metagenomic sequencing - CSF RNA and DNA Illumina MiSeq v1
, 2022 This protocol was developed to evaluate unbiased cerebrospinal fluid metagenomics at a referral centre in South Africa. Commercial kit protocols are generally used unmodified and limited use of in-house developed methods or reagents is made. This maintains the simplicity and accessibility of the protocol which can serve as a base for evaluation of ...
Gert Marais +8 more
openaire +1 more source
Spaced seeds improve k-mer-based metagenomic classification [PDF]
, 2015 Metagenomics is a powerful approach to study genetic content of environmental samples that has been strongly promoted by NGS technologies. To cope with massive data involved in modern metagenomic projects, recent tools [4, 39] rely on the analysis of k ...
Brinda, Karel +2 more
core +2 more sources
Metagenomic data of free cyanide and thiocyanate degrading bacterial communities
Data in Brief, 2017 The data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater.
Lukhanyo Mekuto +4 more
doaj +1 more source
Genomic catastrophes frequently arise in esophageal adenocarcinoma and drive tumorigenesis [PDF]
, 2014 Oesophageal adenocarcinoma (EAC) incidence is rapidly increasing in Western countries. A better understanding of EAC underpins efforts to improve early detection and treatment outcomes.
Anderson, M +43 more
core +4 more sources
2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador [PDF]
, 2017 Background: Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru.
Andersson, Björn +10 more
core +4 more sources
A metagenomic survey of lamb's pre- and post-weaning fecal microbiomes
Journal of Taibah University for Science, 2020 Weaning is known to be the most stressful and critical transition experienced by lambs, it affects the structure of the gastrointestinal tract microbiomes, which significantly influences the lamb’s health and performance. The present study identified the
N. N. Albakri +2 more
doaj +1 more source
Complete genome sequences of three African foot-and-mouth disease viruses from clinical samples isolated in 2009 and 2010 [PDF]
, 2016 <p>The complete genome sequences of three foot-and-mouth disease viruses (one virus of each serotype SAT1, SAT2 and O) were directly sequenced from RNA extracted from clinical bovine samples, demonstrating the feasibility of full-genome sequencing ...
De Clercq, Kris +8 more
core +1 more source
Microbial communities and arsenic biogeochemistry at the outflow of an alkaline sulfide-rich hot spring. [PDF]
, 2016 Alkaline sulfide-rich hot springs provide a unique environment for microbial community and arsenic (As) biogeochemistry. In this study, a representative alkaline sulfide-rich hot spring, Zimeiquan in the Tengchong geothermal area, was chosen to study ...
Dai, Xinyue +9 more
core +1 more source
Environmental DNA (eDNA) 16S metabarcoding Illumina MiSeq NGS PCR Protocol v2 [PDF]
, 2018 This protocol is aimed at amplifying the 16S rRNA hypervariable region 4 (16S V4) in prokaryotes. The primers (515fBand 806rB, 926R) used in this protocol are based on the primer utilized in Apprill et al 2015 and the Earth Microbiome Project (EMP). This work was supported by NASA grant NNX14AP62A ‘National Marine Sanctuaries as Sentinel Sites for a ...
Collin Closek +9 more
openaire +2 more sources