Results 41 to 50 of about 142,852 (247)
Agronomy, 2021 High-throughput amplicon sequencing that primarily targets the 16S ribosomal DNA (rDNA) (for bacteria and archaea) and the Internal Transcribed Spacer rDNA (for fungi) have facilitated microbial community discovery across diverse environments.
Ko-Hsuan Chen +3 more
semanticscholar +1 more source
Genomics Data, 2017 Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
doaj +1 more source
Annals of Microbiology, 2021 To investigate the diversity of the epiphytic bacteria on corn (Zea mays) and alfalfa (Medicago sativa) collected in Hengshui City and Xingtai City, Hebei Province, China, and explore crops suitable for natural silage.
Meixiao Wu +6 more
semanticscholar +1 more source
PLoS ONE, 2017 Metagenetics is a rapid and taxonomically comprehensive method for revealing community structures within environmental samples, based on large amounts of sequence data produced by high-throughput sequencers.
Junya Hirai +2 more
doaj +1 more source
Microbiome, 2014 To take advantage of affordable high-throughput next-generation sequencing technologies to characterize microbial community composition often requires the development of improved methods to overcome technical limitations inherent to the sequencing ...
D. Fadrosh +6 more
semanticscholar +1 more source
Bacterial diversity and community in Qula from the Qinghai–Tibetan Plateau in China [PDF]
PeerJ, 2018 Qula is a cheese-like product usually prepared with unpasteurized yak milk under open conditions, with both endogenous and exogenous microorganisms involved in the fermentation process.
Yan Zhu +7 more
doaj +2 more sources
Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform
Nucleic Acids Research, 2015 With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs.
M. Schirmer +5 more
semanticscholar +1 more source
Scientific Reports, 2021 Environmental DNA (eDNA) analysis is a method of detecting DNA from environmental samples and is used as a biomonitoring tool. In recent studies, Illumina MiSeq has been the most extensively used tool for eDNA metabarcoding.
Ryohei Nakao +5 more
doaj +1 more source
Metagenomic investigation of faecal microbiota in sheep and goats of the same ages
Journal of Taibah University for Science, 2021 Despite the significant influence of the microbial communities that inhabit the ruminant’s gastrointestinal tract on their health and production, the study of the composition and variations in sheep and goats gut microbiome is limited.
I. I. Shabana +2 more
doaj +1 more source
cpn60 metagenomic amplicon library preparation for the Illumina Miseq platform
, 2021 Abstract This protocol can be applied to determine the composition of a microbial community. The cpn60 gene (also known as groEL, hsp60) is present in almost all bacteria and a 552-558 bp region of the gene has been established as a barcode for species level identification of bacteria. The primer cocktail used in this
Champika Fernando, Janet E. Hill
openaire +1 more source