Results 61 to 70 of about 139,474 (279)

SARS-CoV-2 Illumina MiSeq protocol v.2 v2

, 2021
ARTIC amplicon sequencing protocol adapted from Josh Quick's https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn for illumina sequencing of SARS-CoV-2
openaire   +1 more source

Genome‐Wide Association Study Reveals Insect Genetics and Microbial Symbiont Effects on Susceptibility of Diaphorina citri to the Citrus Greening Pathogen, Candidatus Liberibacter Asiaticus

Advanced Science, EarlyView.
This study investigates the genetic and microbial factors influencing the susceptibility of Diaphorina citri to the citrus greening pathogen Candidatus Liberibacter asiaticus (CLas), employing a microbiome Genome Wide Association Study. The research identifies a key gene encoding an MFS‐type transporter contributing to CLas infectivity and abundance in
Kai Liu, Qingcui He, Zeyue Lin, Shixuan Huang, Zichun Zhong, Pingyang Zhu, Mengge Gao, Luyao Zhao, Han Jin, Guiting Wu, Gurr M. Geoff, Qunxin Han, Rui Pang   +12 more
wiley   +1 more source

Comparison of Two Massively Parallel Sequencing Platforms using 83 Single Nucleotide Polymorphisms for Human Identification

Scientific Reports, 2017
The potential of Massively Parallel Sequencing (MPS) technology to vastly expand the capabilities of human identification led to the emergence of different MPS platforms that use forensically relevant genetic markers.
Dame Loveliness T. Apaga, Sheila E. Dennis, Jazelyn M. Salvador, Gayvelline C. Calacal, Maria Corazon A. De Ungria   +4 more
doaj   +1 more source

Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats

BioTechniques, 2014
The inverted terminal repeats (ITRs) of adeno-associated virus (AAV) are notoriously difficult to sequence owing to their high GC-content (70%) and palindromic sequences that result in the formation of a very stable, 125 bp long, T-shaped hairpin ...
Karl Petri, Raffaele Fronza, Richard Gabriel, Christine Käppel, Ali Nowrouzi, R. Michael Linden, Els Henckaerts, Manfred Schmidt   +7 more
doaj   +1 more source

Datasets of surface water microbial populations from two anthropogenically impacted sites on the Bhagirathi-Hooghly River

Data in Brief, 2020
The Bhagirathi-Hooghly River, part of the River Ganga, flows along densely urbanized areas in West Bengal, India. The River water is extensively used for household activities, human consumption including bathing, social purposes and multifaceted ...
Anwesha Ghosh, Manojit Debnath, Punyasloke Bhadury   +2 more
doaj   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

5′ Rapid Amplification of cDNA Ends and Illumina MiSeq Reveals B Cell Receptor Features in Healthy Adults, Adults With Chronic HIV-1 Infection, Cord Blood, and Humanized Mice

Frontiers in Immunology, 2018
Using 5′ rapid amplification of cDNA ends, Illumina MiSeq, and basic flow cytometry, we systematically analyzed the expressed B cell receptor (BCR) repertoire in 14 healthy adult PBMCs, 5 HIV-1+ adult PBMCs, 5 cord blood samples, and 3 HIS-CD4/B mice ...
Eric Waltari, Manxue Jia, Caroline S. Jiang, Hong Lu, Jing Huang, Cristina Fernandez, Andrés Finzi, Daniel E. Kaufmann, Daniel E. Kaufmann, Martin Markowitz, Moriya Tsuji, Xueling Wu   +11 more
doaj   +1 more source

Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection [PDF]

, 2018
Nosocomial pathogens can cause life-threatening infections in neonates and immunocompromised patients. E. bugandensis (EB-247) is a recently described species of Enterobacter, associated with neonatal sepsis.
Bunk, Boyke, Chakraborty, Trinad, Doijad, Swapnil Prakash, Falgenhauer, Linda, Goesmann, Alexander, Gwozdzinski, Konrad, Hain, Torsten, Hegemann, Julian D., Imirzalioglu, Can, Jaiswal, Sangeeta, Mannala, Gopala Krishna, Marahiel, Mohamed A., Mraheil, Mobarak Abu, Mshana, Stephen E., Overmann, Jörg, Pati, Niladri Bhusan, Ryan, Daniel, Schultze, Tilman, Spröer, Cathrin, Suar, Mrutyunjay, Yao, Yancheng   +20 more
core   +2 more sources

Dietary Baicalin Supplementation Can Enhance the Growth Performance of Weaned Piglets and Maintain the Intestinal Barrier Integrity

Animal Research and One Health, EarlyView.
This research innovatively illustrates baicalin’s multifaceted mechanisms in enhancing piglets’ intestinal health: modulating bile acid metabolism via probiotics, reinforcing tight junction proteins (ZO‐1/claudin), suppressing TLR4/NF‐κB‐mediated inflammation, whereas promoting growth and reducing diarrhea.
Yuhui Gao, Jie Liu, Rui Yang, Xizhen Fan, Cheng Liang, Xin'e Shi, Jianjun Jin   +6 more
wiley   +1 more source

Effects of Lycium barbarum Residue Substituting Dietary Maize on Fecal Microbiota and Growth Performance of Crossbred Simmental Cattle

Animal Research and One Health, EarlyView.
The bioactive substances we extracted from Lycium barbarum residue (LBR) have a typical polysaccharide structure. When 1.80% LBR was used to replace maize in the diet, the average daily gain of rossbred Simmental was significantly increased, and anti‐inflammatory ability was improved. In addition, LBR improved the fecal microbial composition, increased
Kun Cai, Mingtan Li, Lihang He, Ning Ma, Haoyu Liu, Chi Xu, Xianhua Wu, Zeguang Xing, Qian Xing, Yuankai Qu, Xi Ma   +10 more
wiley   +1 more source