Results 71 to 80 of about 142,852 (247)

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

Advanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son, Goeun Kim, Jungjoon K. Lee   +2 more
wiley   +1 more source

A quantitative and qualitative comparison of illumina MiSeq and 454 amplicon sequencing for genotyping the highly polymorphic major histocompatibility complex (MHC) in a non-model species

BMC Research Notes, 2017
Background High-throughput sequencing enables high-resolution genotyping of extremely duplicated genes. 454 amplicon sequencing (454) has become the standard technique for genotyping the major histocompatibility complex (MHC) genes in non-model organisms.
Haslina Razali, Emily O’Connor, Anna Drews, Terry Burke, Helena Westerdahl   +4 more
doaj   +1 more source

Datasets of surface water microbial populations from two anthropogenically impacted sites on the Bhagirathi-Hooghly River

Data in Brief, 2020
The Bhagirathi-Hooghly River, part of the River Ganga, flows along densely urbanized areas in West Bengal, India. The River water is extensively used for household activities, human consumption including bathing, social purposes and multifaceted ...
Anwesha Ghosh, Manojit Debnath, Punyasloke Bhadury   +2 more
doaj   +1 more source

Comparison of Pre‐ and Postoperative Gut Microbiota Diversity in Patients With Rectal Cancer Undergoing Stoma Creation and Closure

Annals of Gastroenterological Surgery, EarlyView.
We investigated the impact of temporary stoma creation and closure on gut microbiota diversity in rectal cancer patients. 16S rRNA sequencing revealed that microbial diversity significantly decreased postoperatively in the stoma group but remained stable in non‐stoma patients.
Yusuke Suzuki, Wataru Osumi, Kohei Taniguchi, Nahoko Kato‐Kogoe, Shoichi Sakaguchi, Shota Nakamura, Yoshiro Imai, Takashi Nakano, Takaaki Ueno, Sang‐Woong Lee   +9 more
wiley   +1 more source

Enhanced QIAseq DIRECT SARS-CoV-2 Kit for Illumina MiSeq v2

, 2022
This SARS-CoV-2 targeted, a rapid viral RNA library preparation kit compatible with Illumina sequencers, provides an approximately five hour turn-around time from viral RNA sample to sequencing. The kit includes cDNA synthesis, SARS-CoV-2 enrichment, and library amplification and indexing reagents.
Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim   +4 more
openaire   +2 more sources

Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing

Viruses, 2020
Enteroviruses are small RNA viruses that affect millions of people each year by causing an important burden of disease with a broad spectrum of symptoms.
Carole Grädel, Miguel A. Terrazos Miani, Christian Baumann, Maria Teresa Barbani, Stefan Neuenschwander, Stephen L. Leib, Franziska Suter-Riniker, Alban Ramette   +7 more
doaj   +1 more source

Airborne Bacterial Communities of PM2.5 in Beijing-Tianjin-Hebei Megalopolis, China as Revealed by Illumina MiSeq Sequencing: A Case Study

Aerosol and Air Quality Research, 2017
Bacteria are ubiquitous and abundant in the atmosphere and some of them are potential pathogens known to cause diseases or allergies in humans. However, the quantities and compositions of total airborne bacterial community and their relationships with ...
Jingfeng Gao, Xiaoyan Fan, Hongyu Li, Kai-ling Pan   +3 more
semanticscholar   +1 more source

Dietary Baicalin Supplementation Can Enhance the Growth Performance of Weaned Piglets and Maintain the Intestinal Barrier Integrity

Animal Research and One Health, EarlyView.
This research innovatively illustrates baicalin’s multifaceted mechanisms in enhancing piglets’ intestinal health: modulating bile acid metabolism via probiotics, reinforcing tight junction proteins (ZO‐1/claudin), suppressing TLR4/NF‐κB‐mediated inflammation, whereas promoting growth and reducing diarrhea.
Yuhui Gao, Jie Liu, Rui Yang, Xizhen Fan, Cheng Liang, Xin'e Shi, Jianjun Jin   +6 more
wiley   +1 more source

Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish cultures

Aquaculture Reports, 2021
The primary objective of this work was to assess the effect of Bacillus amyloliquefaciens on the microbiota in polyculture tanks stocked with grass carp, gibel carp and sliver carp. The B.
Yibin Yang, Jingjin Xia, Yongtao Liu, Jing Dong, Ning Xu, Qiuhong Yang, Shun Zhou, Xiaohui Ai   +7 more
doaj   +1 more source

Effects of Lycium barbarum Residue Substituting Dietary Maize on Fecal Microbiota and Growth Performance of Crossbred Simmental Cattle

Animal Research and One Health, EarlyView.
The bioactive substances we extracted from Lycium barbarum residue (LBR) have a typical polysaccharide structure. When 1.80% LBR was used to replace maize in the diet, the average daily gain of rossbred Simmental was significantly increased, and anti‐inflammatory ability was improved. In addition, LBR improved the fecal microbial composition, increased
Kun Cai, Mingtan Li, Lihang He, Ning Ma, Haoyu Liu, Chi Xu, Xianhua Wu, Zeguang Xing, Qian Xing, Yuankai Qu, Xi Ma   +10 more
wiley   +1 more source