Results 121 to 130 of about 131,982 (283)

Analysis of hypervariable DNA sequences by NGS technologies: QuasiFlow [PDF]

, 2016
The development of Next Generation Sequencing (NGS) technologies has allowed deep characterization of highly variable sequences such as viral or mitochondrial genomes.
Claros-Diaz, Manuel Gonzalo, Díaz Martínez, Luis, Grande-Perez, Ana, Seoane Zonjic, Pedro, Viguera-Minguez, Enrique   +4 more
core  

Bioengineered Bacterial Vesicles and Biomimetic Hybrids Eliminate Biofilms and Balance the Gut Microbiome

Small, EarlyView.
Biomimetic nanocarriers based on liposomes and Myxobacteria outer‐membrane vesicles (OMVs) are effective antibiotic delivery systems. Hybrids have a higher drug loading and can target intracellular pathogens, while OMVs evade immune clearance. Biomimetic nanoantibiotics effectively eliminate the biofilms of Gram‐negative bacteria. Besides, Myxobacteria
Leila Pourtalebi Jahromi, Benedikt Kronast, Jennifer Munkert, Lorenzo Sana, Marcus Koch, Heike Danzer, Sirka Dormeyer, Shuhan Jiang, Fabian Herrmann, Matthias Weiler, Anna Kashkanova, Vahid Sandoghdar, Mario M. Zaiss, Gregor Fuhrmann   +13 more
wiley   +1 more source

On the ability to extract MLVA profiles of Vibrio cholerae isolates from WGS data generated with Oxford Nanopore Technologies

BMC Research Notes
Objective Multiple-Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) is widely used to subtype pathogens causing foodborne and waterborne disease outbreaks.
Jérôme Ambroise, Bertrand Bearzatto, Jean-Francois Durant, Leonid M. Irenge, Jean-Luc Gala   +4 more
doaj   +1 more source

Isolation and characterization of novel microsatellite loci for the Eastern Pacific marine sponge Mycale cecilia by Illumina MiSeq sequencing. [PDF]

Mol Biol Rep, 2023
Hernández-Lozano MY, Cruz-Barraza JA, Rocha-Olivares A.   +2 more
europepmc   +1 more source

Distinct Nasal Microbiome Profiles and Prediction Model for Allergic Rhinitis, Nonallergic Rhinitis, and Healthy Children

World Journal of Otorhinolaryngology - Head and Neck Surgery, EarlyView.
The alpha and beta diversity of the nasal microbiome differed among children with allergic rhinitis (AR), nonallergic rhinitis (NAR), and healthy controls (HCs). Compared to HC, AR had more Escherichia‐Shigella, Negativicoccus, and Campylobacter, while NAR had more Dolosigranulum and fewer Enterobacteriaceae.
Kantima Kanchanapoomi, Iyarit Thaipisuttikul, Perapon Nitayanon, Witchaya Srisuwatchari, Punchama Pacharn, Nualanong Visitsunthorn, Orathai Jirapongsananuruk   +6 more
wiley   +1 more source

OPTIMIZATION OF ILLUMINA® NEXTERA™ XT LIBRARY PREPARATION FOR THE MITOCHONDRIAL GENOME SEQUENCING AND CONFIRMATORY SANGER SEQUENCING

Medicinski Glasnik
Aim: Due to increasing use of mitochondrial DNA (mtDNA) sequencing in both forensic practice and clinical disease research, this study explores the optimization of the next-generation sequencing (NGS) method for whole mitochondrial genome analysis on the
Nejira Handžić, Dino Pećar, Selma Durgut, Naida Mulahuseinović, Ivana Čeko, Adna Ašić, Mirza Izmirlija, Sabina Šegalo, Lana Salihefendić, Rijad Konjhodžić   +9 more
doaj   +1 more source

Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis

African Journal of Laboratory Medicine, 2019
South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic ...
Shaheed V. Omar, Lavania Joseph, Halima M. Said, Farzana Ismail, Nabila Ismail, Thabisile L. Gwala, Nazir A. Ismail   +6 more
doaj   +1 more source

Effect of Processing Treatment and Modified Atmosphere Packing on Carrot's Microbial Community Structure by Illumina MiSeq Sequencing. [PDF]

Molecules, 2022
Ratajczak K, Staninska-Pięta J, Czarny J, Cyplik P, Wolko Ł, Piotrowska-Cyplik A.   +5 more
europepmc   +1 more source

Bacterial Community Sequences of Submerged Aquatic Vegetation in the Potomac River. [PDF]

, 2020
Here, we report results from PCR and sequencing of bacterial 16S rRNA genes from leaf and root surfaces from nine submerged aquatic vegetation (SAV) samples comprising five species.
Alexiev, Alexandra, Eisen, Jonathan A, Lang, Jenna M, Vann, Laura E   +3 more
core  

Candida auris sequencing by Illumina miSeq using Illumina DNA Prep v1

Candida auris sequencing is becoming more important for public health and surveillance. This protocol is designed to guide individuals experienced in sequencing in setting up a SOP for sequencing C. auris. This protocol is designed for Illumina short-read sequencing.
openaire   +1 more source